Targeting cancer stem cells by melatonin: Effective therapy for cancer treatment

Maroufi, Nazila Fathi; Vahedian, Vahid; Hemati, Saideh; Rashidi, Mohammad‐Reza; Akbarzadeh, Maryam; Zahedi, Mohammadsaeid; Pouremamali, Farhad; Isazadeh, Alireza; Taefehshokr, Sina; Hajazimian, Saba; Seraji, Nikou; Nouri, Mohammad

doi:10.1016/j.prp.2020.152919

Cited by 45 publications

(34 citation statements)

References 82 publications

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“…More than 3500 years ago, natural resources such as plants had been used to treatment of various diseases (12). Modern pharmacy science has moved considerably toward medicinal herbs and treatment of many diseases, including cancer, using bio-active compounds over the past two decades (13). Uses of these medicinal plants in the treatment of cancer were increased due to their anticancer effects, fewer side effects, low costs, and especially its ability to target different signaling pathways (14).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Colorectal Cancer Cell Line CaCo-2 by Essential Oil of Eucalyptus camaldulensis Through Induction of Apoptosis

Taheri¹,

Ghorbani²,

Safi³

et al. 2020

ACTA

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Treatment of colorectal cancer is one of the important challenges due to the increase of resistance to chemotherapeutic drugs. Isolated natural compounds from medicinal plants and other sources often are used as novel drugs for treatment of various human cancer. The aim of this study was to investigate the antioxidant and anticancer activity of Eucalyptus camaldulensis essential oil on colorectal cancer cell line Caco-2. The antioxidant activity of extracted E. camaldulensis essential oil (1000, 800, 400, 200, 100, 50, 25, 12.5, 6, and 3 μg/mL) was evaluated by free radicals inactivation method. Moreover, MTT assay was used to examine the cytotoxic effects of E. camaldulensis essential oil on the Caco-2 cell line. The mRNA expression of BAX and BCL-2 genes was studied using quantitative Real-Time PCR method, in treated cancer cells compared to untreated cells. We indicated a significant, impressive antioxidant activity in 1000 μg/mL of E. camaldulensis essential oil, in a concentration-dependent manner. In addition, we found that this product exerted a cytotoxic effect on cancer cells when 100 μg/mL concentration was considered as half-maximal inhibitory concentration (IC50). Also, the expression of BAX and BCL-2 genes were significantly upregulated and downregulated, respectively, in the treated Caco-2 cells with E. camaldulensis essential oil. In conclusion, our study showed significant antioxidant and anticancer activity in E. camaldulensis essential oil in a concentration and time-dependent manner, which may be due to the reduction of free radicals and induction of apoptosis process in colorectal cancer cells.

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Section: Discussionmentioning

confidence: 99%

Inhibition of Colorectal Cancer Cell Line CaCo-2 by Essential Oil of Eucalyptus camaldulensis Through Induction of Apoptosis

Taheri¹,

Ghorbani²,

Safi³

et al. 2020

ACTA

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“…Our study showed that the CC cells viability decreased as time and concentration dependent manner after treatment with conditioned medium (38, 40, 42, 44, and 46%) and cell lysate (11,12,13,14, and 15%).…”

Section: Cancer Cells Viabilitymentioning

confidence: 57%

“…Inhibition of apoptosis is an important pathological processes in patients with CC, which caused by induction of anti-apoptotic genes as well as suppression of pre-apoptotic genes expression [12]. The dysregulated proliferation of cancer cells and resistance to apoptosis are two important causes of cancer progression [13].…”

Section: Discussionmentioning

confidence: 99%

“…The CC cells (1×10 4 /well) were seeded in 96-well plate containing culture medium supplemented by FBS. After 24 hours, the cancer cells were treated with conditioned medium (38, 40, 42, 44, and 46%) and cell lysate (11,12,13,14, and 15%), and incubated for 72 hours. The Tetrazolium Micro-culture (MTT) method was performed according to the manufacturer's instruction.…”

Section: Cancer Cells Viability Assaymentioning

confidence: 99%

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Inhibition of Colorectal Cancer Cells HT-29 by Secretome and Extraction of Human Umbilical Cord Wharton’s Jelly Stem Cells

Jadid

Emami-Naeini

Goleij³

et al. 2021

Preprint

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Background and aim: Colorectal cancer (CC) is aggressive cancer and the major cause of death worldwide that need the development of novel and effective therapeutic methods. Recently suggested that the human Wharton’s jelly stem cells (hWJSCs) have an anti-proliferation activity against of several cancer cells through apoptosis induction. Therefore, this study aimed to investigate the effects of conditioned medium and cell lysate of human umbilical cord hWJSCs against the HT-29 cancer cell line and mechanisms of apoptosis induction.Methods: In this study, the hWJSCs conditioned medium and cell lysate were prepared from 10 human umbilical cord samples. The effects of hWJSCs conditioned medium and cell lysate were evaluated on the viability, migration, invasion, and apoptosis of HT-29 CC cell line. The expression of apoptosis related BAX, BCL2, SMAC, SURVIVIN, and Cas9 genes were evaluated in CC cells treated with hWJSCs conditioned medium and cell lysate.Results: We observed that conditioned medium and cell lysate of hWJSCs decrease CC cells viability, proliferation, migration, and invasion in a concentration- and time-dependent manner. Moreover, conditioned medium and cell lysate increased apoptosis rate of CC cells, which can be due to increase BAX, SMAC, and Cas9 genes, as well as decrease BCL2 and SURVIVIN genes.Conclusions: Our study suggest that conditioned medium and cell lysate of hWJSCs can inhibit CC cells through induction of apoptosis. However, further studies on are required to more accurate results.

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“…The chemotherapy following surgical method has been used for treatment of patients with advanced PC. However, chemotherapy is associated with several side effects and poor survival [23,24]. In order to improve the overall survival of patients with advanced PC, many studies combined various chemotherapy drugs with different natural agents, although the results were not encouraging [25,26].…”

Section: Discussionmentioning

confidence: 99%

Enhanced Anticancer Potency of Hydroxytyrosol and Curcumin by PLGA-PAA Nano-Encapsulation on PANC-1 Pancreatic Cancer Cell Line

Farhoudi

Shadman

Chavoshi

et al. 2020

Preprint

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Background: Pancreatic cancer (PC) is a highly aggressive malignancy associated with low survival rates. Many chemotherapeutic regimens have been investigated for advanced unresectable and metastatic PC, but with only minimal improvement in survival and prognosis. The present study aimed to investigate the anti-cancer function of free and nano-encapsulated hydroxytyrosol (Hyd) and curcumin (Cur), and its combinations (Hyd-Cur) on the PANC-1 cell line.Methods: The poly lactide-co-glycolide-co-polyacrylic acid (PLGA-co-PAA) nano-encapsulated Hyd and Cur were synthesized, and MTT assay was performed to evaluate cytotoxic effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur. Moreover, effects of free and nano-encapsulated Hyd, Cur, and Hyd-Cur were evaluated on viability, migration, morphological alterations, colony formation, and apoptosis on PANC-1 cell line. The mRNA expression levels of MMP2, MMP9, BAX, BCL-2, and Cas9 genes were assessed after treated PANC-1 cells with free and nano-encapsulated Hyd, Cur, and Hyd-Cur.Results: The obtained results showed that free and nano-encapsulated Hyd, Cur, and Hyd-Cur treatments significantly decreased the viability, migration, and colony formation in the PANC-1 cells. Furthermore, apoptosis rates in PANC-1 cells were increased in a concentration and time dependent manner in all of the treatment groups. Moreover, anti-proliferative activity of nano-encapsulated Hyd-Cur was significantly more than other treatments.Conclusions: According to our results, Hyd-Cur combination and nano-encapsulation therapy exerts more profound apoptotic and anti-proliferative effects on PANC-1 cell line than free Hyd or Hyd monotherapy.

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Targeting cancer stem cells by melatonin: Effective therapy for cancer treatment

Cited by 45 publications

References 82 publications

Inhibition of Colorectal Cancer Cell Line CaCo-2 by Essential Oil of Eucalyptus camaldulensis Through Induction of Apoptosis

Inhibition of Colorectal Cancer Cell Line CaCo-2 by Essential Oil of Eucalyptus camaldulensis Through Induction of Apoptosis

Inhibition of Colorectal Cancer Cells HT-29 by Secretome and Extraction of Human Umbilical Cord Wharton’s Jelly Stem Cells

Enhanced Anticancer Potency of Hydroxytyrosol and Curcumin by PLGA-PAA Nano-Encapsulation on PANC-1 Pancreatic Cancer Cell Line

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