Targeting cell plasticity for regeneration: From in vitro to in vivo reprogramming

Romanazzo, Sara; Kang, Lin; Srivastava, Pallavi; Kilian, Kristopher A.

doi:10.1016/j.addr.2020.08.007

Cited by 10 publications

(7 citation statements)

References 245 publications

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“…Interestingly, in an Arabidopsis, leaf-derived iPSC culture consisting in 85% mesophyll cells, hence a priori relatively homogenous, only 5% cells seem to contribute an effective regeneration process (Xu et al, 2021). This is reminiscent from animal iPSC cultures showing variable levels of cellular reprogramming (0.5-10%, occasionally >50%) depending on the tissue source and inductive method (Kalra et al, 2021; Romanazzo et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Yet recently, single cell-based reconstructions demonstrated a high heterogeneity of transcriptome patterns even in a relatively homogenous plant iPSC culture consisting in 85% mesophyll cells (Xu et al, 2021). Cell-to-cell variability, particularly in animal system, has been recognized as an important factor controlling the inherent properties of a cellular system prompting investigations to understand its origin and regulation (Eldar & Elowitz, 2010;Eling et al, 2019;Guillemin & Stumpf, 2021;Huang, 2009;Mitchell & Hoffmann, 2018;Mojtahedi et al, 2016;Pelkmans, 2012;Richard & Yvert, 2014;Safdari et al, 2020). One approach to measure the heterogeneity, or information content, of biological systems is based on the calculation of the Shannon entropy borrowed from statistical mechanics (Gandrillon et al, 2021;MacArthur & Lemischka, 2013).…”

Section: A Rapid Multiscale Reorganisation Of Chromatin Which Traject...mentioning

confidence: 99%

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Multiscale chromatin dynamics and high entropy in plant iPSC ancestors

Rutowicz,

Lüthi,

de Groot

et al. 2023

Preprint

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Protoplasts, derived from plant tissues, are considered functional equivalent of animal induced pluripotent stem cells (iPSC). Similarly, to their animal counterparts, plant iPSC show large-scale, chromatin reorganisation, thought to be a hallmark of pluripotency acquisition. To capture in more details the nuclear morphology and chromatin organisation of plant iPSC, we deployed high- throughput imaging and a custom, supervised image analysis protocol extracting over 100 chromatin features. Our study unravels a rapid, multiscale dynamics of chromatin patterns which trajectory strongly depends on nutrients availability. We measured a high heterogeneity of chromatin features indicating an intrinsic entropy of iPSC cultures, which reduced over time. We found that entropy reduction is antagonistically controlled by phytohormones and intrinsic epigenetic factors.

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Section: Discussionmentioning

confidence: 99%

Section: A Rapid Multiscale Reorganisation Of Chromatin Which Traject...mentioning

confidence: 99%

Multiscale chromatin dynamics and high entropy in plant iPSC ancestors

Rutowicz,

Lüthi,

de Groot

et al. 2023

Preprint

View full text Add to dashboard Cite

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“…Interesting improvements in next generation gene insertion strategies and RNA gated payload expression could be potential game changers for the field, although they also require improvements to delivery (Burgess, 2022;Jiang et al, 2022;Srivastava & DeWitt, 2016;Yarnall et al, 2022). In several cases, groups have observed effective delivery using a variety of different nanoparticles for in vivo reprogramming (Ofenbauer & Tursun, 2019;Romanazzo et al, 2020). (Huang et al, 2018;Schoenfeldt et al, 2022;Tang & Cheng, 2017).…”

Section: Limitati On S and Future Per S Pec Tive S For Ivprmentioning

confidence: 99%

Partial cellular reprogramming: A deep dive into an emerging rejuvenation technology

Paine,

Nguyen,

Ocampo

2023

Aging Cell

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Aging and age‐associated disease are a major medical and societal burden in need of effective treatments. Cellular reprogramming is a biological process capable of modulating cell fate and cellular age. Harnessing the rejuvenating benefits without altering cell identity via partial cellular reprogramming has emerged as a novel translational strategy with therapeutic potential and strong commercial interests. Here, we explore the aging‐related benefits of partial cellular reprogramming while examining limitations and future directions for the field.

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“…Although two-dimensional (2D) cell culture has been a well-accepted method to culture cells in vitro for more than a century and has significantly contributed to the understanding of cell behavior, it does not provide a vivo-like environment; therefore, physical cues, cell–cell and cell–matrix communication, and the interplay of different cell types are difficult to reproduce. In contrast, the 3D culture technique can overcome these difficulties and even provide mechanical cell reprogramming without genetic or biochemical interventions [ 9 ]. Such 3D culture systems can be classified into scaffold-free or anchorage-independent 3D culture systems, anchorage-dependent or scaffold-based 3D culture systems, and specialized 3D culture platforms.…”

Section: Introductionmentioning

confidence: 99%

Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture

García

Uribe

López

et al. 2022

Cells

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Cell spheroids are applied in various fields of research, such as the fabrication of three-dimensional artificial tissues in vitro, disease modeling, stem cell research, regenerative therapy, and biotechnology. A preclinical 3D culture model of primary human gingival fibroblasts free of external factors and/or chemical inducers is presented herein. The ultrastructure of the spheroids was characterized to establish a cellular model for the study of periodontal tissue regeneration. The liquid overlay technique was used with agarose to generate spheroids. Fibroblasts in 2D culture and cell spheroids were characterized by immunofluorescence, and cell spheroids were characterized by optical and scanning electron microscopy, energy-dispersive X-ray spectroscopy, electron backscatter diffraction, and Fourier transform infrared spectroscopy. Osteogenically related genes were analyzed by RT-qPCR. Gingival fibroblasts formed spheroids spontaneously and showed amorphous calcium phosphate nanoparticle deposits on their surface. The results suggest that human gingival fibroblasts have an intrinsic potential to generate a mineralized niche in 3D culture.

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Targeting cell plasticity for regeneration: From in vitro to in vivo reprogramming

Cited by 10 publications

References 245 publications

Multiscale chromatin dynamics and high entropy in plant iPSC ancestors

Multiscale chromatin dynamics and high entropy in plant iPSC ancestors

Partial cellular reprogramming: A deep dive into an emerging rejuvenation technology

Ultrastructural Characterization of Human Gingival Fibroblasts in 3D Culture

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