Targeting effect of berberine on type I fimbriae of Salmonella Typhimurium and its effective inhibition of biofilm

Xu, Chenran; Wang, Feiying; Huang, Fangfang; Yang, Min; He, Dinggeng; Deng, Le

doi:10.1007/s00253-021-11116-1

Cited by 23 publications

(15 citation statements)

References 38 publications

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“…Fimbriae play an important role in the adhesion of Salmonella spp. to the target cell (Xu et al., 2021), and cellulose provides biological, mechanical, and chemical protection against the environment (Čabarkapa et al., 2019).…”

Section: Principal Textmentioning

confidence: 99%

Salmonella spp. in low water activity food: Occurrence, survival mechanisms, and thermoresistance

Morasi

Rall

Dantas

et al. 2022

Journal of Food Science

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The occurrence of disease outbreaks involving low‐water‐activity (aw) foods has gained increased prominence due in part to the fact that reducing free water in these foods is normally a measure that controls the growth and multiplication of pathogenic microorganisms. Salmonella, one of the main bacteria involved in these outbreaks, represents a major public health problem worldwide and in Brazil, which highlights the importance of good manufacturing and handling practices for food quality. The virulence of this pathogen, associated with its high ability to persist in the environment, makes Salmonella one of the main challenges for the food industry. The objectives of this article are to present the general characteristics, virulence, thermoresistance, control, and relevance of Salmonella in foodborne diseases, and describe the so‐called low‐water‐activity foods and the salmonellosis outbreaks involving them.

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Section: Principal Textmentioning

confidence: 99%

Salmonella spp. in low water activity food: Occurrence, survival mechanisms, and thermoresistance

Morasi

Rall

Dantas

et al. 2022

Journal of Food Science

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“…According to the existing research, the antibacterial effect of berberine can be generally summarized as “multi-effect” (Karaosmanoglu et al, 2014 ). Berberine can inhibit the formation of biofilm (Zhang et al, 2020 ; Bhatia et al, 2021 ; Xu et al, 2021 ), destroy the cell wall and cell membrane (Karaosmanoglu et al, 2014 ; Zhang et al, 2020 ; Xia et al, 2022 ), inhibit bacterial cell division (Domadia et al, 2008 ), inhibit nucleic acid and protein synthesis (Du et al, 2020 ), and inhibit bacterial adhesion (Kang et al, 2015 ), etc.…”

Section: Discussionmentioning

confidence: 99%

A New Perspective on the Antimicrobial Mechanism of Berberine Hydrochloride Against Staphylococcus aureus Revealed by Untargeted Metabolomic Studies

Yang

Hong

et al. 2022

Front. Microbiol.

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Berberine hydrochloride (BBR) is a natural product widely used in clinical medicine and animal production. It has a variety of antimicrobial effects, but its complex antimicrobial mechanism has not been clarified. This study aimed to discover the metabolic markers and gain a new perspective on the antibacterial mechanism of BBR. The effects of different inhibitory concentrations of BBR on the survival and growth of standard strain Staphylococcus aureus ATCC 25923 were analyzed by the bacteriostatic activity test. Differences in intracellular metabolites of S. aureus following 19 μg/ml BBR exposure for 1 h were investigated by combining non-targeted metabolomics techniques of gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). The results showed that the minimum inhibitory concentration of BBR against S. aureus was 51 μg/ml. A total of 368 and 3,454 putative metabolites were identified by GC-MS and LC-MS analyses, respectively. Principal component analysis showed the separation of intracellular metabolite profiles between BBR-exposed samples and non-exposed controls. Pathway activity profiling analysis indicated a global inhibition of metabolisms by BBR exposure, while enhancement was also found in nucleic acid metabolism, amino sugar, and nucleotide sugar metabolism. Several metabolic markers were screened out mainly based on their variable importance of projection values. Two pyridine dicarboxylic acids were significantly downregulated, suggesting the reduction of stress resistance. The oxidized phospholipid (PHOOA-PE) was accumulated, while lipid antioxidant gamma-tocopherol was decreased, and farnesyl PP, the synthetic precursor of another antioxidant (staphyloxanthin), was decreased below the detection threshold. This evidence indicates that BBR reduced the antioxidant capacity of S. aureus. Accumulation of the precursors (UDP-GlcNAc, CDP-ribitol, and CDP-glycerol) and downregulation of the key metabolite D-Ala-D-Ala suggest the inhibition of cell wall synthesis, especially the peptidoglycan synthesis. Metabolites involved in the shikimate pathway (such as 3-dehydroshikimate) and downstream aromatic amino acid synthesis were disturbed. This study provides the first metabolomics information on the antibacterial mechanism of BBR against S. aureus. The key metabolic markers screened in this study suggest that the shikimate pathway, staphyloxanthin synthesis, and peptidoglycan biosynthesis are new directions for further study of BBR antibacterial mechanism in the future.

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“…Berberine is an alkaloid that has been widely used as an anti-infective agent in traditional medicine. It possesses antibacterial activities against a wide range of microorganisms alone or in combination with antibiotics routinely [ 210 , 211 , 212 ].…”

Section: Preclinical and Clinical Studies On Antibacterial Effects Of Phytochemicalsmentioning

confidence: 99%

Phytochemicals: A Promising Weapon in the Arsenal against Antibiotic-Resistant Bacteria

et al. 2021

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The extensive usage of antibiotics and the rapid emergence of antimicrobial-resistant microbes (AMR) are becoming important global public health issues. Many solutions to these problems have been proposed, including developing alternative compounds with antimicrobial activities, managing existing antimicrobials, and rapidly detecting AMR pathogens. Among all of them, employing alternative compounds such as phytochemicals alone or in combination with other antibacterial agents appears to be both an effective and safe strategy for battling against these pathogens. The present review summarizes the scientific evidence on the biochemical, pharmacological, and clinical aspects of phytochemicals used to treat microbial pathogenesis. A wide range of commercial products are currently available on the market. Their well-documented clinical efficacy suggests that phytomedicines are valuable sources of new types of antimicrobial agents for future use. Innovative approaches and methodologies for identifying plant-derived products effective against AMR are also proposed in this review.

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Targeting effect of berberine on type I fimbriae of Salmonella Typhimurium and its effective inhibition of biofilm

Cited by 23 publications

References 38 publications

Salmonella spp. in low water activity food: Occurrence, survival mechanisms, and thermoresistance

Salmonella spp. in low water activity food: Occurrence, survival mechanisms, and thermoresistance

A New Perspective on the Antimicrobial Mechanism of Berberine Hydrochloride Against Staphylococcus aureus Revealed by Untargeted Metabolomic Studies

Phytochemicals: A Promising Weapon in the Arsenal against Antibiotic-Resistant Bacteria

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