Targeting Epstein-Barr virus nuclear antigen 1 (EBNA-1) with Murraya koengii bio-compounds: An in-silico approach

Mathivadani, V; Smiline, A S; Priyadharsini, J. Vijayashree

doi:10.4149/av_2020_111

Cited by 77 publications

(71 citation statements)

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“…The fruits are considered to be fusiform-ellipsoid shape, slightly beaked and marked with white streaks when immature and a bright scarlet when fully ripe [15]. Our team has extensive knowledge and research experience that has translated into high quality publications [16][17][18][19][20][21][22][23][24][25][26][27], [28][29][30][31][32]. [33][34] [35].…”

Section: Introductionmentioning

confidence: 99%

Attenuation of Quorum Sensing Controlled Virulence Factors and Biofilm Formation by Edible Fruit Extract of Coccinia indica against Pseudomonas aeruginosa

Devi

Ganesh

Girija

et al. 2021

JPRI

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Background: Pseudomonas aeruginosa is a Gram-negative opportunistic human pathogen that mainly infects immunocompromised individuals and patients with urinary tract infection and chronic infections of the respiratory pathways, including cystic fibrosis. Many quorum sensing (QS) controlled components such as bio surfactants and swarming motilities play an important role in the establishment of biofilms. Targeting these factors through anti-QS strategies prevent biofilm formation and treating infections. Coccinia indica commonly called little gourd is used to treat diabetes, wound, burn infections and has antioxidant, antibacterial and antitussive properties. Methods: The methanolic fruit extract of C. indica was prepared and screened for anti-QS and anti-biofilm formation activity. Pyocyanin inhibition, rhamnolipid, crystal violet staining assay tests was performed and the extract was observed under fluorescent microscope. Results: The results obtained are as follows - the fruit extract inhibits the pyocyanin at 58.13% and 42.27% at 0.5 mg/ml and 1.0 mg/ml, biofilm at 69.86% and 49.06% at 0.5 mg/ml and 1.0 mg/ml, inhibits rhamnolipid assay and under fluorescent microscope it is seen scattered whereas control produce biofilm matrix like appearance. Conclusion: Since less study has been made on the quorum sensing and biofilm activity of C.indica our study aimed to fulfil it and it was found that it exhibits good biofilm formation and thus can be used for treating infections.

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Section: Introductionmentioning

confidence: 99%

Attenuation of Quorum Sensing Controlled Virulence Factors and Biofilm Formation by Edible Fruit Extract of Coccinia indica against Pseudomonas aeruginosa

Devi

Ganesh

Girija

et al. 2021

JPRI

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“…Therefore, there is a need for a search of an alternate antidiabetic agent [19]. Evidence has shown that copper nanoparticles synthesised from medicinal plant Dioscorea bulbifera tuber extract showed antioxidant and antidiabetic activity [20].Our team has extensive knowledge and research experience that has translate into high quality publications [21][22][23][24][25][26][27][28][29][30][31][32], [33][34][35][36][37]. [38] [39] [40].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis of Copper Nanoparticles using Mucuna Pruriens and its Antioxidant and Antidiabetic Activity

Ali

Geetha

Rajeshkumar

2021

JPRI

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Introduction: The field of Nanotechnology has gained importance since last century. Nanoparticles can be used in medicine due to its increased interaction with microbes and has less side effects than drugs. Antioxidant compounds scavenge free radicals and inhibit the oxidative mechanisms that lead to degenerative diseases. There is a growing number of diabetes patients all over the world. Wide varieties of synthetic drugs are being used for the treatment of Type 2 diabetes mellitus, most of them possess side effects in the long run such as hepatotoxicity, abdominal pain, flatulence and diarrhea. Therefore, there is a need for a search of an alternate antidiabetic agent Aim: The aim of the study is to synthesize Copper nanoparticles from Mucuna pruriens and to evaluate its antioxidant and antidiabetic activity. Materials and methods: Plant extract of Mucuna pruriens was prepared and filtered by Whatman No 1 filter paper. Copper sulphate was added to the plant extract and kept in a magnetic stirrer for nanoparticle synthesis. The synthesized nanoparticle was preliminarily analysed using UV visible spectroscopy. Finally the left over solution was taken to calculate antioxidant activity and antidiabetic activity. Results: Antioxidant activity was calculated by DPPH method and the percentage of inhibition of copper nanoparticles synthesised from Mucuna pruriens was 58.5% for 10µL, 59.6% for 20µL, 67.5% for 30µL, 71.4% for 40µL and 72.3% for 50µL. Antidiabetic activity was calculated by alpha-amylase inhibitory assay and the percentage of inhibition of copper nanoparticles synthesised from Mucuna pruriens was 66% for 10µL, 69% for 20µL, 73% for 30µL, 79% for 40µL and 80% for 50µL. Conclusion: We can conclude that copper nanoparticles synthesised from Mucuna pruriens are a potent antioxidant and antidiabetic agent. Since it shows a good activity in free radical scavenging, copper nanoparticles can be used in a clinical therapeutic application and also in the management of type 2 diabetes mellitus.

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“…Computational techniques are widely employed to screen for drug molecules and their targets in microbes and host. Several studies have been initiated by the authors previously to deduce the potential targets of synthetic [12] and phytocompounds [13], [14] against red complexes and other common dental pathogens. Virtual screening methods have been used to reduce cost and time.…”

Section: Introductionmentioning

confidence: 99%

Virtual Screening to Identify the Protein Network Interactions of Triclosan with Streptococcus mutans and Enterococcus faecalis

Hariprasanth

Priyadharsini

Girija

et al. 2021

JPRI

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Introduction: Triclosan is considered to be an important ingredient in toothpastes and mouth rinses. Several studies have reported contradictory results regarding the antimicrobial effect of triclosan. Hence, the present in silico study intends to identify the potential targets of triclosan in two common dental pathogens Streptococcus mutans and Enterococcus faecalis. Aim: To identify the protein network interactions of triclosan in Streptococcus mutans and Enterococcus faecalis by virtual screening method. Materials and Methods: The STITCH v5.0 database was initially used for identifying drug-protein interactions followed by VICMPred and VirulentPred which was employed to identify functional class of the proteins and its virulence property. Finally, BepiPred v1.0 Linear Epitope Prediction tool was used to identify the potential epitopes of the virulent proteins. Results: Triclosan was found to interact with crucial proteins in S. mutans and E. faecalis which could contribute to severe forms of periodontitis and endodontic diseases. Conclusion: Taken together, the present study provides the preliminary data on the potential targets of triclosan in common dental pathogens. Further experimental validation is warranted to provide concrete evidence on the molecular targets of dental pathogens.

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Targeting Epstein-Barr virus nuclear antigen 1 (EBNA-1) with Murraya koengii bio-compounds: An in-silico approach

Cited by 77 publications

References 0 publications

Attenuation of Quorum Sensing Controlled Virulence Factors and Biofilm Formation by Edible Fruit Extract of Coccinia indica against Pseudomonas aeruginosa

Attenuation of Quorum Sensing Controlled Virulence Factors and Biofilm Formation by Edible Fruit Extract of Coccinia indica against Pseudomonas aeruginosa

Biosynthesis of Copper Nanoparticles using Mucuna Pruriens and its Antioxidant and Antidiabetic Activity

Virtual Screening to Identify the Protein Network Interactions of Triclosan with Streptococcus mutans and Enterococcus faecalis

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