Targeting Gut Bacteria Using Inulin‐Conjugated Mesoporous Silica Nanoparticles

Baeckmann, Cornelia von; Riva, Alessandra; Guggenberger, Patrick; Kählig, Hanspeter; Han, Seung Won; Inan, Deniz; Favero, Giorgia Del; Berry, David; Kleitz, Freddy

doi:10.1002/admi.202102558

Cited by 5 publications

(5 citation statements)

References 62 publications

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“…A similar behavior was reported by von Baeckmann et al in the case of the grafting of inulin on other types of MSNs. [34] On the other hand, in the case of the conjugation via carbamate formation (DMSN-NCO-man), the mannose attachment did not form pronounced particle interconnections (Figure 1B-iii,iv).…”

Section: Mannosylation Of Dendritic Mesoporous Silica Nanoparticles (...mentioning

confidence: 94%

MANNosylation of Mesoporous Silica Nanoparticles Modifies TLR4 Localization and NF‐κB Translocation in T24 Bladder Cancer Cells

Hohagen,

Sánchez,

Herbst

et al. 2024

Adv Healthcare Materials

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D‐mannose is widely used as non‐antibiotic treatment for bacterial urinary tract infections. This application is based on a well‐studied mechanism of binding to the type 1 bacterial pili and, therefore, blocking bacteria adhesion to the uroepithelial cells. To implement D‐mannose into carrier systems, the mechanism of action of the sugar in the bladder environment is also relevant and requires investigation. Herein, two different MANNosylation strategies using mesoporous silica nanoparticles (MSNs) are described. The impact of different chemical linkers on bacterial adhesion and bladder cell response is studied via confocal microscopy imaging of the MSN interactions with the respective organisms. Cytotoxicity is assessed and the expression of Toll‐like receptor 4 (TLR4) and caveolin‐1 (CAV‐1), in the presence or absence of simulated infection with bacterial lipopolysaccharide (LPS), is evaluated using the human urinary bladder cancer cell line T24. Further, localisation of the transcription factor NF‐κB due to the MANNosylated materials is examined over time. The results show that MANNosylation modifies bacterial adhesion to the nanomaterials and significantly affects TLR4, caveolin‐1, and NF‐κB in bladder cells. These elements are essential components of the inflammatory cascade/pathogens response during urinary tract infections. These findings demonstrate that MANNosylation is a versatile tool to design hybrid nanocarriers for targeted biomedical applications.

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Section: Mannosylation Of Dendritic Mesoporous Silica Nanoparticles (...mentioning

confidence: 94%

MANNosylation of Mesoporous Silica Nanoparticles Modifies TLR4 Localization and NF‐κB Translocation in T24 Bladder Cancer Cells

Hohagen,

Sánchez,

Herbst

et al. 2024

Adv Healthcare Materials

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“…In order to identify members of the gut microbiota with inulin-binding characteristics, we employed uorescently-labeled mesoporous silica nanoparticles (MSNs) grafted with inulin 30,31 to capture and sort inulin-bound bacteria. We performed anaerobic incubations of freshly collected human stool samples from six different donors and incubated them for one hour in the presence of inulin-grafted or ungrafted MSNs (Fig.…”

Section: Detection Of Inulin-binding Bacteria In the Gut Microbiotamentioning

confidence: 99%

“…Mobile composition of matter number forty-eight (MCM-48)-type mesoporous silica nanoparticles with a diameter of 150 nm were synthesized according to previous work 30,31 . After the synthesis, the material was calcinated at 550 °C for 5 h to remove the template.…”

Section: Synthesis Of Silica Nanoparticlesmentioning

confidence: 99%

“…Immediately before sorting, samples were ltered with a 35 mm nylon mesh using BD tubes 12 X 75 mm (BD, Germany) and analyzed using the cell sorter BD FACS Melody(BD, Germany) equipped with BD FACSChorus software (BD) as previously described 31,36 . By using FACS we aimed to select the bacteria population that bind the MSNs.…”

Section: Fluorescence Activated Cell Sorting (Facs)mentioning

confidence: 99%

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Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting

Berry,

Riva,

Rasoulimehrabani

et al. 2023

Preprint

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Prebiotics are defined as non-digestible dietary components that promote the growth of beneficial gut microorganisms. In many cases, however, this capability is not systematically evaluated. Here, we develop a methodology for determining prebiotic-responsive bacteria using the popular dietary supplement inulin. We first identify microbes with a capacity to bind inulin using mesoporous silica nanoparticles functionalized with inulin. 16S rRNA gene amplicon sequencing of sorted cells revealed that the ability to bind inulin was widespread in the microbiota. We further evaluate which taxa are metabolically stimulated by inulin and find that diverse taxa from the phyla Firmicutes and Actinobacteria respond to inulin, and several isolates of these taxa can degrade inulin. Incubation with another prebiotic, xylooligosaccharides (XOS), in contrast, shows a more robust bifidogenic effect. Interestingly, the Coriobacteriia Eggerthella lenta and Gordonibacter urolithinfaciens are indirectly stimulated by the inulin degradation process, expanding our knowledge of inulin-responsive bacteria.

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“…[37][38][39][40] Another very attractive characteristic of MCM materials is the fact that these materials consist only of silica (SiO 2 ), which is non-toxic for our body, so the application in and on our body is possible. [41][42][43] Therefore, MCM materials are also perfect platforms for biomedical applications. [44,45] However, the material and biological interactions should not be neglected, especially in case of nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

Assembly of Tailored Porous Nanofibers from Mesoporous MCM‐41 Nanoparticles via Electrospinning

2023

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For the first time the assembly of isolated and purified MCM‐41 nanoparticles into nanofibers was realized by electrospinning. Electrospinning was conducted with a dispersion of these nanoparticles and polyvinylpyrrolidone in ethanol. The nanofibers were characterized by electron microscopy, X‐ray diffraction, elemental analysis, gas physisorption and infrared spectroscopy in diffuse reflectance. The variation of the spinning parameters was investigated in detail towards the influence on the nanofiber diameter. The change of the applied voltage and the flow rate show strong impacts on the nanofiber diameter. Therefore, the nanofiber diameter can be tailored by choosing suitable electrospinning parameters. The fiber mats with thin nanofibers were then additionally functionalized with sulfonic acid groups in a proof of principal study. The nanofiber morphology was maintained both after functionalization with thiol group and subsequent oxidation to sulfonic acid groups.

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Targeting Gut Bacteria Using Inulin‐Conjugated Mesoporous Silica Nanoparticles

Cited by 5 publications

References 62 publications

MANNosylation of Mesoporous Silica Nanoparticles Modifies TLR4 Localization and NF‐κB Translocation in T24 Bladder Cancer Cells

MANNosylation of Mesoporous Silica Nanoparticles Modifies TLR4 Localization and NF‐κB Translocation in T24 Bladder Cancer Cells

Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting

Assembly of Tailored Porous Nanofibers from Mesoporous MCM‐41 Nanoparticles via Electrospinning

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