Targeting Insulin Amyloid Assembly by Aminosugars and Their Derivatives

Nagaveni, V.; Sravani, M.; Prabhakar, S.; Sreedhar, B.; Vairamani, M.

doi:10.2174/092986611795222687

Cited by 3 publications

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“…For compounds like sugars that have low BAs, it is difficult to compare mass spectrometry data with absolute solution-phase binding properties, but the present data provide reasonable BAs and are also helpful in understanding the physicochemical properties of protein and ligand interactions in the gas phase in the absence of solvent. In our recent report on targeting amino sugars for insulin aggregation 34 using an assay method based on mass spectrometry, we have found that all of the amino sugars studied promote fibril growth, but in the case of GalNH 2 fibril formation was less. The reasons for the exceptional behaviour of GalNH 2 are still not clear.…”

Section: Effect Of Ligand Concentration Ph and Incubation Timementioning

confidence: 89%

Differential Interactions of Isomeric Amino Sugars with Insulin Studied under Electrospray Ionisation Mass Spectrometry

Nagaveni

Sravani

Prabhakar

et al. 2011

Eur J Mass Spectrom (Chichester)

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Protein-ligand interactions were studied for bovine insulin-amino sugar systems under electrospray ionisation mass spectrometry conditions. The isomeric amino sugars showed differences in the relative abundance of 1:1 protein-ligand complex formation. The electrospray ionisation and tandem mass spectrometry results of the complex clearly demonstrated that the differences in the interaction of isomeric sugars with insulin are mainly due to the differences in their gas-phase basicity. The same phenomenon is replicated in the formation of complexes between insulin and other ligands, such as amino acids, as well as in the binding of the amino sugars with amyloid β 1-40 peptide.

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Section: Effect Of Ligand Concentration Ph and Incubation Timementioning

confidence: 89%

Differential Interactions of Isomeric Amino Sugars with Insulin Studied under Electrospray Ionisation Mass Spectrometry

Nagaveni

Sravani

Prabhakar

et al. 2011

Eur J Mass Spectrom (Chichester)

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“…Using an ESI-based screening assay, the inhibitory effects of amino sugars were evaluated and the results were compared with transmission electron microscopy imaging. 460 She found that insulin fibrillation is reduced in the presence of galactosamine when compared to glucosamine and mannosamine.…”

Section: Protein Chemistry and Proteomicsmentioning

confidence: 99%

Mass Spectrometry in India

Vairamani

Prabhakar

2012

Eur J Mass Spectrom (Chichester)

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All rights reserved EUROPEAN JOURNAL OF MASS SPECTROMETRY This review highlights the research in mass spectrometry carried out all over India, with a focus on the last two decades. There has been a phenomenal increase in the use of mass spectrometers in India during the last decade following the introduction, at the beginning of the 21st century, of rugged electrospray ionization sources for the commercially available instruments, with applications such as pharmacokinetics and bio-equivalence drug studies, pesticide residue analysis and proteomics. There have been more papers published in these areas than could be fully covered in this article. This review covers mostly research in mass spectrometry carried out continuously in institutions where advanced facilities for mass spectrometry are functional. Researchers in India, with some notable exceptions, have always used and still use commercial mass spectrometers in their various areas of research, rather than develop their unique instruments. The review is divided into three parts: organic, elemental (or atomic) and biological mass spectrometry. Organic mass spectrometry Organic and bio-organic chemistry Research in organic mass spectrometry in the early years was mainly focused on the study of mass spectral fragmentation of different classes of new compounds,and was initiated in the 1960s by the late Das and his colleagues from the National Chemical Laboratory (NCL), Pune. The first double-focusing mass spectrometer (CEC-110 B) was installed at NCL for high-resolution mass measurements. The compounds studied by Das and his colleagues included aromatic amides, Benzophenanthridine derivatives, norses

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Injection of insulin amyloid fibrils in the hippocampus of male Wistar rats: report on memory impairment and formation of amyloid plaques

et al. 2015

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Amyloid fibrils result from a particular type of protein aggregation, and have been linked with various disorders, including neurodegenerative ones. In the case of Alzheimer's disease, amyloid beta (abeta) fibrils are detected in patients' brain, in the amyloid plaques. These fibrils can be produced in vitro, and their injection into animals' brains generates an animal model of Alzheimer's disease. Based on the structural similarity of amyloid fibrils that are formed from different proteins, we hypothesized that injecting insulin amyloid fibrils into rats' brains could result in amyloid plaque formation. Fourteen male Wistar rats were divided into control and experimental groups (n = 7). The experimental group was bilaterally injected with insulin amyloid in the hippocampus. Seven days after injection, a shuttle box test was performed and the experimental group's memory was found to be impaired. Histological investigation of these rats' brain showed the formation of amyloid plaques in the hippocampus. A limited test has provided preliminary evidence for the stability of these plaques up to 35 days. Further complementary studies are required to fully validate the proposed procedure, which is simple and relatively low cost, and could be suggested as an alternative to models generated with abeta fibrils.

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Targeting Insulin Amyloid Assembly by Aminosugars and Their Derivatives

Cited by 3 publications

References 0 publications

Differential Interactions of Isomeric Amino Sugars with Insulin Studied under Electrospray Ionisation Mass Spectrometry

Differential Interactions of Isomeric Amino Sugars with Insulin Studied under Electrospray Ionisation Mass Spectrometry

Mass Spectrometry in India

Injection of insulin amyloid fibrils in the hippocampus of male Wistar rats: report on memory impairment and formation of amyloid plaques

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