Targeting lipid-sensing nuclear receptors PPAR (α, γ, β/δ): HTVS and molecular docking/dynamics analysis of pharmacological ligands as potential pan-PPAR agonists

Comparative bioactivity assessment of bixin pigment and associated phytochemicals extracted from annatto seeds using conventional and green solvents

“…After 24 h of incubation with the test compounds, the cell was treated with a mixture of 9 parts growth media and 1-part MTT dye (5 mg/mL) and further incubated for 3 h. Following the incubation period, the media containing the MTT dye was removed, and the purple-colored formazan crystals were dissolved by adding DMSO. The absorbance of the resulting solution was measured at 570 nm using (Multiskan FC, Thermo Scientific, DE) [ 34 ].…”

“…To assess the antioxidant activity of bixin and annatto extracts, the thiobarbituric acid reactive substances (TBARS) assay was performed in cultured cells in vitro as described previously [ 34 ]. This assay measures the levels of malondialdehyde (MDA), as an indicator of lipid peroxidation and oxidative damage in the test samples.…”

Biochemical evaluation of wound healing efficacy of cold plasma-conditioned media under different operational conditions

“…To evaluate the antioxidant activity of different GI and GII groups and time intervals, the TBA reactive substances assay was conducted in vitro using cultured cells as described previously [28]. This assay measures the levels of malondialdehyde (MDA), as an indicator of lipid peroxidation and oxidative damage in cell culture media exposed to (cold) PCM.…”

“…The resulting mixture was heated in a boiling water bath for 15 min to generate a pink-coloured adduct. The absorbance of the adduct was measured spectrophotometrically at 530 nm (Multiskan FC, Thermo Scientific, DE) [28]. The obtained values were then compared to a standard MDA curve to quantify the level of MDA in different C-APPJ exposed cell culture media, in µM mg −1 .…”