Targeting mediodorsal thalamic CB1 receptors to inhibit dextromethorphan-induced anxiety/exploratory-related behaviors in rats: The post-weaning effect of exercise and enriched environment on adulthood anxiety

Banaei-Boroujeni, Golnoush; Rezayof, Ameneh; Alijanpour, Sakineh; Nazari-Serenjeh, Farzaneh

doi:10.1016/j.jpsychires.2022.11.035

Cited by 2 publications

(4 citation statements)

References 83 publications

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“…Moreover, oscillations of the intensity of the Earth's potential may be important for setting the biological clocks regulating diurnal body rhythms, such as cortisol secretion [51]. The results of the study were consistent with previous studies [18,51].…”

Section: Discussionsupporting

confidence: 90%

“…The rats were individually placed in the central platform facing a closed arm, and they were allowed to explore the maze for a 5-min test period. The duration of time spent in the open and closed arms, and activity of each arm were the behavioral measures that were recorded for each rat [17][18][19][20][21][22]. The apparatus was wiped clean with a damp sponge and dried with paper towels between tests.…”

Section: Elevated Plus Maze (Epm)mentioning

confidence: 99%

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The Effect of Earthing Mat on Stress-Induced Anxiety-like Behavior and Neuroendocrine Changes in the Rat

Park

Jeong

et al. 2022

Biomedicines

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Grounding is a therapeutic technique that involves doing activities that “ground” or electrically reconnect us to the earth. The physiological effects of grounding have been reported from a variety of perspectives such as sleep or pain. However, its anti-stress efficacy is relatively unknown. The present study investigated the stress-related behavioral effects of earthing mat and its neurohormonal mechanisms in the Sprague–Dawley male rat. Rats were randomly divided into four groups: the naïve normal (Normal), the 21 days immobilization stressed (Control), the 21 days stressed + earthing mat for 7 days (A7) or 21 days (A21) group. The depressive-and anxiety like behaviors were measured by forced swimming test (FST), tail suspension test (TST) and elevated plus maze (EPM). Using immunohistochemistry, the expression of corticotrophin-releasing factor (CRF) and c-Fos immunoreactivity were analyzed in the brain. In the EPM, time spent in the open arm of the earthing mat groups was significantly increased compared to the Control group (p < 0.001), even though there were without effects among groups in the FST and TST. The expression of CRF immunoreactive neurons in the earthing mat group was markedly decreased compared to the Control group. Overall, the earthing mat reduced stress-induced behavioral changes and expression of c-Fos and CRF immunoreactivity in the brain. These results suggest that the earthing mat may have the potential to improve stress-related responses via the regulation of the corticotrophinergic system.

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Section: Discussionsupporting

confidence: 90%

Section: Elevated Plus Maze (Epm)mentioning

confidence: 99%

The Effect of Earthing Mat on Stress-Induced Anxiety-like Behavior and Neuroendocrine Changes in the Rat

Park

Jeong

et al. 2022

Biomedicines

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“…Existing evidence supports the positive impact of EE on both brain and behavior [41,56], demonstrating its efficacy in mitigating deficits associated with various brain-related disorders or exposure to adverse conditions throughout life [40,57]. While studies support that EE increases CB 1 expression and activation in the brain and that these alterations are associated with reduced anxiety [37,45], there is currently a lack of evidence concerning the beneficial impact of EE on alterations in the endogenous cannabinoid system caused by CUS. To our knowledge, the current study is the first to explore the potential interaction between EE, initiated during adolescence, and CUS in adulthood, on the expression of CB 1 receptors in three distinct subregions of the hippocampus in both male and female rodents.…”

Section: Discussionmentioning

confidence: 99%

“…These EE-associated alterations have been implicated in the reduction in stressrelated behavior [44]. In general, the increases in CB 1 expression and activation in the brain as a result of EE exposure have been associated with reduced anxiety [37,45]. While the involvement of CB 1 receptors in the stress response has been well documented [20][21][22] and EE has been shown to increase their expression [44], there is currently a lack of evidence concerning the beneficial effects of EE in the endogenous cannabinoid system against the negative effects caused by CUS.…”

Section: Introductionmentioning

confidence: 99%

Sex-Specific Differences and the Role of Environmental Enrichment in the Expression of Hippocampal CB1 Receptors following Chronic Unpredictable Stress

Dandi,

Kesidou,

Simeonidou

et al. 2024

Brain Sciences

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Stress-related mental disorders have become increasingly prevalent, thus endangering mental health worldwide. Exploring stress-associated brain alterations is vital for understanding the possible neurobiological mechanisms underlying these changes. Based on existing evidence, the brain endogenous cannabinoid system (ECS) plays a significant role in the stress response, and disruptions in its function are associated with the neurobiology of various stress-related disorders. This study primarily focuses on investigating the impact of chronic unpredictable stress (CUS) on the expression of hippocampal cannabinoid type 1 (CB1) receptors, part of the ECS, in adult male and female Wistar rats. Additionally, it explores whether environmental enrichment (EE) initiated during adolescence could mitigate the CUS-associated alterations in CB1 expression. Wistar rats, shortly after weaning, were placed in either standard housing (SH) or EE conditions for a duration of 10 weeks. On postnatal day 66, specific subgroups of SH or EE animals underwent a 4-week CUS protocol. Western blot (WB) analysis was conducted in the whole hippocampus of the left brain hemisphere to assess total CB1 protein expression, while immunohistochemistry (IHC) was performed on the right hemisphere to estimate the expression of CB1 receptors in certain hippocampal areas (i.e., CA1, CA3 and dentate gyrus-DG). The WB analysis revealed no statistically significant differences in total CB1 protein levels among the groups; however, reduced CB1 expression was found in specific hippocampal sub-regions using IHC. Specifically, CUS significantly decreased CB1 receptor expression in the CA1 and DG of both sexes, whereas in CA3 the CUS-associated decrease was limited to SH males. Interestingly, EE housing proved protective against these reductions. These findings suggest a region and sex-specific endocannabinoid response to chronic stress, emphasizing the role of positive early experiences in the protection of the adolescent brain against adverse conditions later in life.

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Targeting mediodorsal thalamic CB1 receptors to inhibit dextromethorphan-induced anxiety/exploratory-related behaviors in rats: The post-weaning effect of exercise and enriched environment on adulthood anxiety

Cited by 2 publications

References 83 publications

The Effect of Earthing Mat on Stress-Induced Anxiety-like Behavior and Neuroendocrine Changes in the Rat

The Effect of Earthing Mat on Stress-Induced Anxiety-like Behavior and Neuroendocrine Changes in the Rat

Sex-Specific Differences and the Role of Environmental Enrichment in the Expression of Hippocampal CB1 Receptors following Chronic Unpredictable Stress

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