Targeting of Lin−Sca+ hematopoietic stem cells with bispecific antibodies to injured myocardium

Lum, Lawrence G.; Fok, Hubert H.; Sievers, Richard E.; Abedi, Mehrdad; Quesenberry, Peter J.; Lee, Randall J.

doi:10.1016/j.bcmd.2003.09.019

Cited by 30 publications

(25 citation statements)

References 11 publications

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“…SDF-1 enhances the clonal growth of mature HPCs in synergy with various cytokines (4,5), whereas SDF-1 alone does not (1,6). Transgenic expression of SDF-1 enhances myeloid progenitor cell survival in vitro and enhanced myelopoiesis in vivo (5,7). SDF-1 treatment of CD34 ϩ cells in vitro also improved cell survival under conditions of growth factor deprivation (5).…”

Section: Introductionmentioning

confidence: 92%

“…Transgenic expression of SDF-1 enhances myeloid progenitor cell survival in vitro and enhanced myelopoiesis in vivo (5,7). SDF-1 treatment of CD34 ϩ cells in vitro also improved cell survival under conditions of growth factor deprivation (5). Recently, it has been demonstrated that SDF-1 is also involved in the maintenance and proliferation of primitive hematopoietic cells (8,9).…”

Section: Introductionmentioning

confidence: 97%

“…In addition, SDF-1 has been shown to be involved in the regulation of hematopoiesis. SDF-1 enhances the clonal growth of mature HPCs in synergy with various cytokines (4,5), whereas SDF-1 alone does not (1,6). Transgenic expression of SDF-1 enhances myeloid progenitor cell survival in vitro and enhanced myelopoiesis in vivo (5,7).…”

Section: Introductionmentioning

confidence: 97%

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Overexpression of Stromal Cell–derived Factor-1 Enhances Endothelium-supported Transmigration, Maintenance, and Proliferation of Hematopoietic Progenitor Cells

Hwang

Kim²,

Park³

et al. 2006

Stem Cells and Development

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To clarify the direct effects of aberrant overexpression of stromal cell-derived factor-1 (SDF-1) by the human endothelium on circulating progenitor cells, we overexpressed the SDF-1 gene in human umbilical vein endothelial cells using an adenoviral vector (HUVEC/AdeSDF-1) and examined the endothelium-supported trafficking and growth of hematopoietic progenitor cells (HPCs) in mobilized peripheral blood (mPB). In culture, the HUVEC/AdeSDF-1 monolayers induced the migration of mPB CD34(+) cells underneath the endothelium within a few hours, whereas HUVEC monolayers that expressed the LacZ gene (HUVEC/AdeLacZ) did not have this effect. In the Transwell system, the HUVEC/AdeSDF-1 cells supported a higher level of spontaneous transmigration of mPB CD34(+) cells than did the HUVEC/AdeLacZ cells. The co-culturing of mPB CD34(+) cells with HUVEC/ AdeSDF-1 cells led to a greater expansion of CD45(+) cells and colony-forming cells and reduced cellular apoptosis. Furthermore, the co-culturing of mPB CD34(+) cells with HUVEC/AdeSDF-1 cells led to the formation of numerous cobblestone-like areas, whereas co-cultures of mPB CD34(+) cells and HUVEC/AdeLacZ supported only a few cobblestone-like areas. These results indicate that SDF- 1 produced by endothelial cells plays an important role not only in the transmigration but also in the growth of HPCs that are in contact with endothelial cells. Our findings suggest that the enhanced expression and production of SDF-1 in the endothelium are essential steps for stem cell or progenitor cell recruitment to specific tissues and for the maintenance of these cells in situ.

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Section: Introductionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 97%

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Overexpression of Stromal Cell–derived Factor-1 Enhances Endothelium-supported Transmigration, Maintenance, and Proliferation of Hematopoietic Progenitor Cells

Hwang

Kim²,

Park³

et al. 2006

Stem Cells and Development

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“…A BiAb, anti-c-kit, and anti-VCAM-1, was produced by chemically heteroconjugating antimouse c-kit to anti-mouse VCAM-1 [Lum et al, 2004b]. Lin-Scaþ cells from bone marrow suspensions of C57BL/6 mice were produced by depleting the lineage positive cells using a cocktail containing rat anti-Ter119, B220, MAC-1, GR-1, Lyt-2, and L3T4 monoclonal antibodies.…”

mentioning

confidence: 99%

“…The c-kit Â anti-VCAM-1 BiAbarmed lin-Scaþ cells or unarmed control linScaþ cells labeled with carboxyfluorescein diacetate succinimidyl diester (CFSE) dye were injected into mice via the jugular vein 24 h after the left anterior descending arteries (LAD) of the mice had been ligated. The mice were euthanized 24 h after the IV injection, the MIs were sectioned, and the infarct area from the mouse that received 100,000 armed lin-Scaþ cell showed markedly increased numbers of CFSEþ cells compared to the infarcts of the control mouse that had received the same number of unarmed lin-Scaþ cells [Lum et al, 2004b]. Subsequently, we generated a BiAb that would target an organ specific injury protein, myosin light chain (MLC), on injured myocardium [Mair et al, 1994;Lyn et al, 2000] by producing anti-human CD45 Â anti-MLC (MLCBi).…”

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confidence: 99%

Virtual reality of stem cell transplantation to repair injured myocardium

Lum

Padbury

Davol

et al. 2005

J of Cellular Biochemistry

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The search for the fountain of youth continues into the 21st century with hopes that embryonic or hematopoietic stem cells (SC) will repair injured tissues in the heart, lungs, pancreas, muscles, nerves, liver, or skin. This commentary focuses on the potential of SC for inducing cardiac regeneration after myocardial injury, the barriers to SC treatment that need to be overcome for ensuring successful cardiac repair, and the experimental approaches that can be applied to the problem. J. Cell. Biochem. 95: 869-874, 2005. ß 2005 Wiley-Liss, Inc.

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A New Era of Cardiac Cell Therapy: Opportunities and Challenges

Huang

Cheng

2018

Adv Healthcare Materials

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Myocardial infarction (MI), caused by coronary heart disease (CHD), remains one of the most common causes of death in the United States. Over the last few decades, scientists have invested considerable resources on the study and development of cell therapies for myocardial regeneration after MI. However, due to a number of limitations, they are not yet readily available for clinical applications. Mounting evidence supports the theory that paracrine products are the main contributors to the regenerative effects attributed to these cell therapies. The next generation of cell-based MI therapies will identify and isolate cell products and derivatives, integrate them with biocompatible materials and technologies, and use them for the regeneration of damaged myocardial tissue. This review discusses the progress made thus far in pursuit of this new generation of cell therapies. Their fundamental regenerative mechanisms, their potential to combine with other therapeutic products, and their role in shaping new clinical approaches for heart tissue engineering, are addressed.

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Targeting of Lin−Sca+ hematopoietic stem cells with bispecific antibodies to injured myocardium

Cited by 30 publications

References 11 publications

Overexpression of Stromal Cell–derived Factor-1 Enhances Endothelium-supported Transmigration, Maintenance, and Proliferation of Hematopoietic Progenitor Cells

Overexpression of Stromal Cell–derived Factor-1 Enhances Endothelium-supported Transmigration, Maintenance, and Proliferation of Hematopoietic Progenitor Cells

Virtual reality of stem cell transplantation to repair injured myocardium

A New Era of Cardiac Cell Therapy: Opportunities and Challenges

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