Targeting of meta-iodobenzylguanidine to SK-N-SH human neuroblastoma xenografts: Tissue distribution, metabolism and therapeutic efficacy

Rutgers, Marja; Buitenhuis, Corine K.M.; Hoefnagel, C.A.; Pa, Voûte; Smets, L. A.

doi:10.1002/1097-0215(20000801)87:3<412::aid-ijc16>3.0.co;2-x

Cited by 20 publications

(21 citation statements)

References 28 publications

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“…As proof-of-principle for X-ray beam delivery, tumour growth was measured over time. As expected for the radiosensitive neuroblastoma, tumour regression was observed almost immediately which confirms the accuracy of the MRI-guided radiotherapy method [30, 33]. …”

Section: Discussionsupporting

confidence: 78%

An efficient and robust MRI-guided radiotherapy planning approach for targeting abdominal organs and tumours in the mouse

Kersemans¹,

Beech²,

Gilchrist³

et al. 2017

PLoS ONE

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IntroductionPreclinical CT-guided radiotherapy platforms are increasingly used but the CT images are characterized by poor soft tissue contrast. The aim of this study was to develop a robust and accurate method of MRI-guided radiotherapy (MR-IGRT) delivery to abdominal targets in the mouse.MethodsA multimodality cradle was developed for providing subject immobilisation and its performance was evaluated. Whilst CT was still used for dose calculations, target identification was based on MRI. Each step of the radiotherapy planning procedure was validated initially in vitro using BANG gel dosimeters. Subsequently, MR-IGRT of normal adrenal glands with a size-matched collimated beam was performed. Additionally, the SK-N-SH neuroblastoma xenograft model and the transgenic KPC model of pancreatic ductal adenocarcinoma were used to demonstrate the applicability of our methods for the accurate delivery of radiation to CT-invisible abdominal tumours.ResultsThe BANG gel phantoms demonstrated a targeting efficiency error of 0.56 ± 0.18 mm. The in vivo stability tests of body motion during MR-IGRT and the associated cradle transfer showed that the residual body movements are within this MR-IGRT targeting error. Accurate MR-IGRT of the normal adrenal glands with a size-matched collimated beam was confirmed by γH2AX staining. Regression in tumour volume was observed almost immediately post MR-IGRT in the neuroblastoma model, further demonstrating accuracy of x-ray delivery. Finally, MR-IGRT in the KPC model facilitated precise contouring and comparison of different treatment plans and radiotherapy dose distributions not only to the intra-abdominal tumour but also to the organs at risk.ConclusionThis is, to our knowledge, the first study to demonstrate preclinical MR-IGRT in intra-abdominal organs. The proposed MR-IGRT method presents a state-of-the-art solution to enabling robust, accurate and efficient targeting of extracranial organs in the mouse and can operate with a sufficiently high throughput to allow fractionated treatments to be given.

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Section: Discussionsupporting

confidence: 78%

An efficient and robust MRI-guided radiotherapy planning approach for targeting abdominal organs and tumours in the mouse

Kersemans¹,

Beech²,

Gilchrist³

et al. 2017

PLoS ONE

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“…At sacrifice, the PC12 xenograft size ranged from 2.5-17 mg, and that of the SK-N-SH tumors from 0.05-2.1 g. The larger sizes of the latter did not interfere with our assays, since the [ 125 I]MIBG levels of the SK-N-SH neuroblastoma are independent of its size [26].…”

Section: [ 125 I]mibg Kinetics Of the Sc Xenograftsmentioning

confidence: 99%

“…Mice 5-8 weeks old were used for inoculations with material from SK-N-SH tumors developing after splenic passage as previously described [22,26]. Tumor fragments of approximately 70 mg were then implanted s.c. in the subcostal flanks of recipient animals and the experiments were normally started 5-7 weeks later.…”

Section: Neuroblastomamentioning

confidence: 99%

“…Neuroblastoma and pheochromocytoma s.c. xenografts were induced at the lower dorsum of the nude mice and 5-6 mice were used per treatment group [26]. The pheochromocytoma tumor sizes ranged from 5-45 mg (mean group sizes 15-25 mg) and that of the neuroblastoma from 0.20-0.95 g (mean group sizes 0.5-0.6 g).…”

Section: External Beam Radiotherapymentioning

confidence: 99%

“…Appropriate dilutions were kept on ice or stored at −20°C for 1 or 2 days until injection, procedures which minimally affected the purity of the [ 131 I]MIBG[26], i.e., chromatographic analysis of the solutions demonstrated on average 3.9% free radioiodide (range 2.7-6.0%; 27). Appropriate dilutions were kept on ice or stored at −20°C for 1 or 2 days until injection, procedures which minimally affected the purity of the [ 131 I]MIBG[26], i.e., chromatographic analysis of the solutions demonstrated on average 3.9% free radioiodide (range 2.7-6.0%; 27).…”

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confidence: 99%

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[131I]- and [125I]metaiodobenzylguanidine therapy in macroscopic and microscopic tumors:A comparative study in SK-N-SH human neuroblastoma and PC12 rat pheochromocytoma xenografts

et al. 2000

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Design of Radioiodinated Pharmaceuticals: Structural Features Affecting Metabolic Stability towards in Vivo Deiodination

Cavina

Born²,

Klaren

et al. 2017

Eur J Org Chem

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Radioiodinated pharmaceuticals are convenient tracers for clinical and research investigations because of the relatively long half‐lives of radioactive iodine isotopes (i.e., 123I, 124I, and 131I) and the ease of their chemical insertion. Their application in radionuclide imaging and therapy may, however, be hampered by poor in vivo stability of the C–I bond. After an overview of the use of iodine in biology and nuclear medicine, we present here a survey of the catabolic pathways for iodinated xenobiotics, including their biodistribution, accumulation, and biostability. We summarize successful rational improvements in the biostability and conclude with general guidelines for the design of stable radioiodinated pharmaceuticals. It appears to be necessary to consider the whole molecule, rather than the radioiodinated fragment alone. Iodine radionuclides are generally retained in vivo on sp2 carbon atoms in iodoarenes and iodovinyl moieties, but not in iodinated heterocycles or on sp3 carbon atoms. Iodoarene substituents also have an influence, with increased in vivo deiodination in the cases of iodophenols and iodoanilines, whereas methoxylation and difluorination improve biostability.

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Targeting of meta-iodobenzylguanidine to SK-N-SH human neuroblastoma xenografts: Tissue distribution, metabolism and therapeutic efficacy

Cited by 20 publications

References 28 publications

An efficient and robust MRI-guided radiotherapy planning approach for targeting abdominal organs and tumours in the mouse

An efficient and robust MRI-guided radiotherapy planning approach for targeting abdominal organs and tumours in the mouse

[131I]- and [125I]metaiodobenzylguanidine therapy in macroscopic and microscopic tumors:A comparative study in SK-N-SH human neuroblastoma and PC12 rat pheochromocytoma xenografts

Design of Radioiodinated Pharmaceuticals: Structural Features Affecting Metabolic Stability towards in Vivo Deiodination

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