Targeting of mouse erythrocytes by band 3 crosslinkers

Jordán, J.A.; DeLoach, John R.; Luque, J.; Diez, José C.

doi:10.1016/0304-4165(96)00040-2

Cited by 13 publications

(9 citation statements)

References 27 publications

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“…16,17,26,28 Oxygen binding equilibrium curves of control and crosslinked BS 3 and DTSSP differ signi®cantly at high crosslinker concentration (5 mm). BS 3 -and DTSSP-crosslinked erythrocytes behave differently to native erythrocytes in vivo 27 since crosslinking promotes targeting to liver and removal from the circulation. It has been proposed that BS 3 and DTSSP mainly crosslink band 3 protein in the erythrocyte membrane.…”

Section: Discussionmentioning

confidence: 99%

“…29 This behaviour could be explained in part by the fact that BS 3 and DTSSP react with band 3 protein. 22,23,27 Crosslinking with these two reagents mainly produced oligomerization of band 3 protein 27 and the modi®cation of this protein could produce interaction with haemoglobin. These results can be correlated with those postulated by other authors which indicate interaction between band 3 and hemoglobin in some processes related to erythrocyte ageing.…”

Section: Discussionmentioning

confidence: 99%

“…18,22,23,27 Two different concentrations of bifunctional reagent were used (5 and 0.5 mm). The cell/reagent concentration relationships were 0.85 Â 10 9 cells-ml À 1 to the two reagent concentrations mentioned above.…”

Section: Chemical Crosslinking Of Mouse Erythrocytesmentioning

confidence: 99%

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Influence of oxidation and crosslinking on oxygen binding properties of mouse erythrocytes

Lotero

Jordán

López

et al. 2001

Cell Biochemistry & Function

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Different chemical treatments for mouse erythrocyte modification has been used. Oxidation treatments with Ascorbate/Fe(3+), a system able to react with intracellular proteins, produced a displacement of the O(2) binding equilibrium curve to a higher affinity behaviour with loss of the haemoglobin cooperativity for oxygen binding. Incubation of mouse erythrocytes with diamide showed that at low reagent concentration (0.8 mM) no modification on oxygen binding equilibrium curves was observed. At higher reagent concentration (2.0 mM), an increased affinity and a disappearance of the cooperative behaviour can be observed. Additionally, crosslinking reactions on mouse erythrocytes with band 3 crosslinkers seemed to affect oxygen binding properties when used at a crosslinker concentration of 5 mM. Oxyhaemoglobin levels in crosslinked and diamide-treated erythrocytes are similar to those found in control cells. In contrast, ascorbate/Fe(3+) treatments produced an increment in the proportion of methaemoglobin, decreasing the oxyhaemoglobin levels in these oxidized erythrocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Influence of oxidation and crosslinking on oxygen binding properties of mouse erythrocytes

Lotero

Jordán

López

et al. 2001

Cell Biochemistry & Function

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“…Artificially-aged RBCs are used to stimulate RBC uptake by APC in the liver and spleen. As liver contains a major proportion of tolerogenic APC (Thomson and Knolle, 2010), we treated cell membranes of loaded RBCs with [bis(sulphosuccinimidyl)]suberate (BS3), since this chemical treatment was already deemed to promote liver targeting and stimulate RBC phagocytosis by macrophages (Chiarantini et al, 1995;Jordan et al, 1996). Using this cross-linking treatment, a large proportion of BS3-treated RBCs was delivered in the liver within 2 h of administration.…”

Section: Discussionmentioning

confidence: 99%

Innovative approach in Pompe disease therapy: Induction of immune tolerance by antigen-encapsulated red blood cells

Cremel

Guerin

Campello

et al. 2015

International Journal of Pharmaceutics

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“…Several processes resulting in the oxidation of red blood cells (RBCs) have been described and correlated with cell damage [1–3]. Some authors have also postulated a role of Band 3 protein in RBC removal from circulation [4,5]. Cross‐linking or clustering of Band 3 may also be molecular processes related to RBC damage and phagocytosis of aged cells by cells of the mononuclear phagocytic system (MPS) [6].…”

Section: Introductionmentioning

confidence: 99%

In vitro phagocytosis of carrier mouse red blood cells is increased by Band 3 cross‐linking or diamide treatment

Jordán

Álvarez

Lotero

et al. 2001

Biotech and App Biochem

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Chemical alteration of red blood cells (RBCs) can induce increased phagocytosis of modified cells by macrophages. In this study we have used different chemical treatments for the modification of the mouse red-blood-cell membrane surface, namely oxidant compounds, such as ascorbate/Fe(+2) and diamide [azodicarboxylic acid bis(dimethylamide)], or Band 3-cross-linking reagents. We monitored the phagocytosis of oxidized or Band 3-cross-linked mouse red blood cells by peritoneal macrophages. The extent of phagocytosis of RBCs is not affected by oxidation with ascorbate/Fe(3+), but it is increased (up to 10%) by oxidation with 2 mM diamide. Furthermore, phagocytosis is greatly increased (up to 40%) as a result of cross-linking with either of two Band 3 bifunctional reagents [bis(sulphosuccinimidyl) suberate (BS(3)) and 3,3'-dithiobis(sulphosuccinimidyl propionate) (DTSSP)]. To evaluate targeting towards macrophages of such modified RBCs for therapeutical purposes, we have determined the phagocytosis of Band 3 carrier RBCs loaded with carbonic anhydrase. In this case phagocytosis is high enough (25%) to deliver the enzyme into macrophages. We have also assayed the influence of serum components and IgG on the efficiency of phagocytosis and discuss the possible phagocytosis mechanisms. In the case of BS(3)-cross-linked carrier RBCs, phagocytosis is markedly enhanced (from 12% up to 25%) by serum components. This opens a way for therapeutic application of these carrier RBCs, with special relevance in short-term delivery to cells of the mononuclear phagocytic system.

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Targeting of mouse erythrocytes by band 3 crosslinkers

Cited by 13 publications

References 27 publications

Influence of oxidation and crosslinking on oxygen binding properties of mouse erythrocytes

Influence of oxidation and crosslinking on oxygen binding properties of mouse erythrocytes

Innovative approach in Pompe disease therapy: Induction of immune tolerance by antigen-encapsulated red blood cells

In vitro phagocytosis of carrier mouse red blood cells is increased by Band 3 cross‐linking or diamide treatment

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