Targeting of SPCSV‐<i>RNase3</i> via CRISPR‐Cas13 confers resistance against sweet potato virus disease

Yu, Yan; Pan, Zhiyuan; Wang, Xiao; Bian, Xiaofeng; Wang, Wei-Chi; Qiang, Liang; Kou, Meng; Ji, Hongtao; Li, Yanjuan; Ma, Daifu; Li, Zongyun; Sun, Jian

doi:10.1111/mpp.13146

Cited by 48 publications

(18 citation statements)

References 51 publications

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“…These are further classified into types II, V, and VI: Cas9 (type‐II) and Cas12 (type‐V) proteins are RNA‐guided, DNA‐targeting endonucleases, while type‐IV Cas13 proteins are RNA‐guided and target RNA. Cas13 variants have been deployed in Nicotiana benthamiana , sweet potato, and potato for conferring resistance to RNA viruses (Cao et al., 2021; Mahas et al., 2019; Yu et al., 2022; Zhan et al., 2019; Zhang, Zhao, et al., 2019).…”

Section: Genome‐editing Technologies For Targeted Mutagenesis Applied...mentioning

confidence: 99%

Targeted mutagenesis with sequence‐specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects

2023

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Many of the world's most important crops are polyploid. The presence of more than two sets of chromosomes within their nuclei and frequently aberrant reproductive biology in polyploids present obstacles to conventional breeding. The presence of a larger number of homoeologous copies of each gene makes random mutation breeding a daunting task for polyploids. Genome editing has revolutionized improvement of polyploid crops as multiple gene copies and/or alleles can be edited simultaneously while preserving the key attributes of elite cultivars. Most genome‐editing platforms employ sequence‐specific nucleases (SSNs) to generate DNA double‐stranded breaks at their target gene. Such DNA breaks are typically repaired via the error‐prone nonhomologous end‐joining process, which often leads to frame shift mutations, causing loss of gene function. Genome editing has enhanced the disease resistance, yield components, and end‐use quality of polyploid crops. However, identification of candidate targets, genotyping, and requirement of high mutagenesis efficiency remain bottlenecks for targeted mutagenesis in polyploids. In this review, we will survey the tremendous progress of SSN‐mediated targeted mutagenesis in polyploid crop improvement, discuss its challenges, and identify optimizations needed to sustain further progress.

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Section: Genome‐editing Technologies For Targeted Mutagenesis Applied...mentioning

confidence: 99%

Targeted mutagenesis with sequence‐specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects

2023

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“…Interestingly, this compatible interaction between the two viruses resulted in a nearly 75% editing efficiency (Uranga et al 2021b). The Cas13 nuclease can target single stranded RNA, rather than double stranded DNA targeted by Cas9, leading to interest in applications targeting RNA viruses (Cao et al 2021;Wolter and Puchta 2018;Yu et al 2021). Additionally, deactivated Cas13 (dCas13) can be used for a variety of interesting applications, including RNA virus detection by binding fluorescently-tagged dCas13 to the RNA target (Wolter and Puchta 2018).…”

Section: Available Virus-mediated Crispr/cas9 Plant Genome Editing Toolsmentioning

confidence: 99%

VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants

et al. 2022

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Agricultural production is hampered by disease, pests, and environmental stresses. To minimize yield loss, it is important to develop crop cultivars with resistance or tolerance to their respective biotic and abiotic constraints. Transformation techniques are not optimized for many species and desirable cultivars may not be amenable to genetic transformation, necessitating inferior cultivar usage and time-consuming introgression through backcrossing to the preferred variety. Overcoming these limitations will greatly facilitate the development of disease, insect, and abiotic stress tolerant crops. One such avenue for rapid crop improvement is the development of viral systems to deliver CRISPR/Cas-based genome editing technology to plants to generate targeted beneficial mutations. Viral delivery of genomic editing constructs can theoretically be applied to span the entire host range of the virus utilized, circumventing the challenges associated with traditional transformation and breeding techniques. Here we explore the types of viruses that have been optimized for CRISPR/Cas9 delivery, the phenotypic outcomes achieved in recent studies, and discuss the future potential of this rapidly advancing technology.

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“…Mahas et al [ 30 ] have demonstrated that CasRx/Cas13d mediates high interference compared to Cas13a and Cas13b simultaneously against one or two different RNA viruses. A recent study on Sweet Potato Virus Disease (SPVD) resistance using the CRISPR/Cas13 system demonstrated that LwaCas13a and RfxCas13d exhibited efficient targeting activity against multiple RNA viruses simultaneously [ 44 ].…”

Section: Applications Of Crispr/cas13 Systems In Plantsmentioning

confidence: 99%

Applications of CRISPR/Cas13-Based RNA Editing in Plants

Kavuri

Ramasamy

et al. 2022

Cells

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The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated (Cas) system is widely used as a genome-editing tool in various organisms, including plants, to elucidate the fundamental understanding of gene function, disease diagnostics, and crop improvement. Among the CRISPR/Cas systems, Cas9 is one of the widely used nucleases for DNA modifications, but manipulation of RNA at the post-transcriptional level is limited. The recently identified type VI CRISPR/Cas systems provide a platform for precise RNA manipulation without permanent changes to the genome. Several studies reported efficient application of Cas13 in RNA studies, such as viral interference, RNA knockdown, and RNA detection in various organisms. Cas13 was also used to produce virus resistance in plants, as most plant viruses are RNA viruses. However, the application of CRISPR/Cas13 to studies of plant RNA biology is still in its infancy. This review discusses the current and prospective applications of CRISPR/Cas13-based RNA editing technologies in plants.

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Targeting of SPCSV‐RNase3 via CRISPR‐Cas13 confers resistance against sweet potato virus disease

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution-NonCo mmercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

Cited by 48 publications

References 51 publications

Targeted mutagenesis with sequence‐specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects

Targeted mutagenesis with sequence‐specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects

VIGE: virus-induced genome editing for improving abiotic and biotic stress traits in plants

Applications of CRISPR/Cas13-Based RNA Editing in Plants

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