Targeting of Tumor-Associated Macrophages Made Possible by PEG-Sheddable, Mannose-Modified Nanoparticles

Zhu, Saijie; Niu, Mengmeng; O’Mary, Hannah L.; Cui, Zhengrong

doi:10.1021/mp400216r

Cited by 213 publications

(203 citation statements)

References 51 publications

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“…Nanoparticles or nanocarriers targeting macrophages have been developed and have been demonstrated to be useful for anti-tumor therapy targeting TAMs (18,19). The present study initially aimed to develop the magnetite nanoparticles used in the present study for use as nanoparticles for targeting TAMs.…”

Section: Discussionmentioning

confidence: 99%

Selective depletion of cultured macrophages by magnetite nanoparticles modified with gelatin

Komohara

Kawauchi

Makiyama

et al. 2017

Experimental and Therapeutic Medicine

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Abstract. Previous studies have indicated pro-tumor functions of macrophages in tumor progression in different types of malignant tumors. The detailed mechanisms of cell-cell interaction between macrophages and tumor cells have been investigated by means of in vitro co-culture experiments. The present study developed magnetite nanoparticles modified with gelatin that are specifically engulfed by macrophages and investigated methods to deplete these macrophages in co-culture experiments using a magnet. T98G glioma cell line and human monocyte-derived macrophages were mixed and co-cultured for 2 days. The T98G cells were isolated by depletion of the macrophages using the magnetite nanoparticles. mRNA expression of a number of pro-tumor molecules in the isolated T98G cells, with or without co-culture with macrophages, was then evaluated. The mRNA expression levels of chemokine (CC motif) ligand 2, interleukin-6 and macrophage-colony stimulating factor receptor (M-CSFR) were significantly upregulated in T98G cells by co-culture with macrophages (P<0.01). M-CSFR protein expression was also increased by co-culture with macrophages. The conditioned medium of co-cultured cells increased M-CSFR expression in T98G cells. Magnetite nanoparticles may be a novel tool not only for investigating the unique activation status of tumor cells in co-culture conditions, but also for targeting pro-tumor macrophages in tumor tissues.

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Section: Discussionmentioning

confidence: 99%

Selective depletion of cultured macrophages by magnetite nanoparticles modified with gelatin

Komohara

Kawauchi

Makiyama

et al. 2017

Experimental and Therapeutic Medicine

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“…Tumor-associated stromal cells (TASCs) such as cancer-associated fibroblasts (CAFs) and tumorassociated macrophages (TAMs) had been found to play a key role in promoting cancer progression and metastasis [78][79][80]. CAFs are widely seen in breast and pancreatic cancer [81,82].…”

Section: Stromal Cellsmentioning

confidence: 99%

“…They quantified the uptake amount (in vitro and in vivo fate) by labelling PLGA with FITC when viewed under a fluorescence microscope. This system may use to target drugs like Yondelis and bisphosphonate clodronate to TAMs [80]. Nowadays, various agents for Macrophage-specific PET imaging had been designed [95].…”

Section: Stromal Cellsmentioning

confidence: 99%

Theranostic Probes for Targeting Tumor Microenvironment: An Overview

Sikkandhar

Nedumaran

Ravichandar

et al. 2017

Preprint

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Long gone was the time when tumors were thought to be insular masses of cells, residing independently at specific sites in an organ. Now, researchers gradually realize that tumors interact with the extracellular matrix (ECM), blood vessels, connective tissues and immune cells in their environment, which is now known as the tumor microenvironment (TME). It is found that the interactions between tumors and their surrounding promote tumor growth, invasion and metastasis. The dynamics and diversity of TME cause the tumors to be heterogeneous and thus pose a challenge for cancer diagnosis, drug design and therapy. As TME is significant in enhancing tumor progression, it is vital to identify the different components in the TME. This review explores how different factors in the TME supply tumors with the required growth factors and signaling molecules to proliferate, invade and metastasis. We also examine the development of TME-targeted nanotheranostics over the recent years for cancer therapy, diagnosis and anticancer drug delivery system. This review further discusses the limitations and future perspective of nanoparticle based theranostics when used in combination with current imaging modalities like Optical Imaging, Magnetic Resonance Imaging (MRI) and Nuclear Imaging (PET and SPECT).

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“…The use of biomarkers expressed at the surface of TAMs as a target has been the focus of attention of nanotechnologists. To avoid recognition by peripheral macrophages, some studies have dealt with the shield of surface-decorated nanocarriers using polymers that degrade due to environmental changes in the tumor microenvironment, such as a more acidic pH (Huang et al 2012 ;Zhu et al 2013 ). Biomarkers aberrantly expressed at the surface of TAMs include the mannose receptor (Movahedi et al 2012 ;Locke et al 2012 ;Zhu et al 2013 ), the galactose-type lectin (Mgl) receptor (Huang et al 2012 ), the folate receptor beta ( Nagai et al 2009 ), scavenger receptors such as CD163 ( Etzerodt et al 2012 ), and legumain (Luo et al 2006 ;Gomez-Cabrero et al 2013 ;Zhang et al 2013 ).…”

Section: Nanocarriers Targeted To Tamsmentioning

confidence: 99%