Targeting quantum dots to surface proteins in living cells with biotin ligase

Howarth, Mark; Takao, Keizo; Hayashi, Yasunori; Ting, Alice Y.

doi:10.1073/pnas.0503125102

Cited by 523 publications

(478 citation statements)

References 42 publications

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“…[25][26][27][28] Although the specificity is very high, the large size (20-50 nm) of the conjugated antibody is a frequent concern. [29,30] Another approach is to attach a relatively small lipid to the QD directly to confer binding, [31] however the challenge is to retain aqueous solubility of the modified QD. Here we report a procedure for generating QDs which have affinity for the hydrophobic lipid-rich cell membrane while retaining aqueous solubility.…”

Section: Introductionmentioning

confidence: 99%

Mixed-surface, lipid-tethered quantum dots for targeting cells and tissues

Zhang

Haage

Whitley

et al. 2012

Colloids and Surfaces B: Biointerfaces

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Quantum dots (QDs), with their variable luminescent properties, are rapidly transcending traditional labeling techniques in biological imaging and hold vast potential for biosensing applications. An obstacle in any biosensor development is targeted specificity. Here we report a facile procedure for creating QDs targeted to the cell membrane with the goal of cell-surface protease biosensing. This procedure generates water-soluble QDs with variable coverage of lipid functional groups. The resulting hydrophobicity is quantitatively controlled by the molar ratio of lipids per QD. Appropriate tuning of the hydrophobicity ensures solubility in common aqueous cell culture media and while providing affinity to the lipid bilayer of cell membranes. The reaction and exchange process was directly evaluated by measuring UV-vis absorption spectra associated with dithiocarbamate formation. Cell membrane binding was assessed using flow cytometry and total internal reflection fluorescence imaging with live cells, and tissue affinity was measured using histochemical staining and fluorescence imaging of frozen tissue sections. Increases in cell and tissue binding were found to be regulated by both QD hydrophobicity and surface charge, underlying the importance of QD surface properties in the optimization of both luminescence and targeting capability. the hydrophobicity ensures solubility in common aqueous cell culture media and while providing affinity to the lipid bilayer of cell membranes. The reaction and exchange process was directly evaluated by measuring UV-vis absorption spectra associated with dithiocarbamate formation. Cell membrane binding was assessed using flow cytometry and total internal reflection fluorescence imaging with live cells, and tissue affinity was measured using histochemical staining and fluorescence imaging of frozen tissue sections. Increases in cell and tissue binding were found to be regulated by both QD hydrophobicity and surface charge, underlying the importance of QD surface properties in the optimization of both luminescence and targeting capability. Mixed-surface, Lipid-tethered Quantum Dots for Targeting Cells and Tissues

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Section: Introductionmentioning

confidence: 99%

Mixed-surface, lipid-tethered quantum dots for targeting cells and tissues

Zhang

Haage

Whitley

et al. 2012

Colloids and Surfaces B: Biointerfaces

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“…Similar to other nanoparticles, QDs can be modified via conjugation of various surface molecules for targeted delivery [33,34]. QDs also provide sufficient surface area to attach therapeutic agents for simultaneous drug delivery and in vivo imaging [35] as well as for tissue engineering [36]. In vivo cancer targeting and imaging in living animals by QDs was first demonstrated by Gao and colleagues [37], where both subcutaneous injection of QD-tagged prostate cancer cells and systemic injection of multifunctional QD probes were used to achieve sensitive and multicolor fluorescence imaging of cancer cells.…”

Section: Quantum Dotsmentioning

confidence: 99%

Nanotechnology in Cancer Diagnosis and Treatment

El-Karim¹,

El-Zahar²,

Anwar³

2015

JPP

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Nanotechnology refers to research and technology development at the atomic, molecular, and macromolecular scale, leading to the controlled manipulation and study of structures and devices with length scales in the 1-100 nanometers range. Objects at this scale, such as "nanoparticles," take on novel properties and functions that differ markedly from those seen in the bulk scale. The small size, surface tailor ability, improved solubility, and multifunctional of nanoparticles open many new research avenues for biologists. Nanoparticles are classified into inorganic nanoparticles such as gold nanoparticles and organic nanoparticles such as carbon nanotubes and dendrimers. Different types of nanoparticals such as nanocrystals, liposomes, polymeric nanoparticles, dendrimers, quantum dots and superparamagnetic nanoparticles illustrated different examples of promising nanoparticles for cancer treatment and imaging.

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“…It has been demonstrated that peptide tags can be labeled with biarsenical dyes (tetracysteine), nickel tris-nitriloacetic acid-conjugated dyes (oligohistidine) (see Table 2 for references), Texas Red ("fluorettes"), or biotin (biotin ligase acceptor peptides). Biotin ligase acceptor peptides are particularly advantageous because biotin ligases recognize cognate acceptor peptides with high specificity, and the use of different biotin ligases with orthogonal recognition specificities permits multiplex labeling [37,38]. Somewhat larger tags, derived from specific receptors and enzymes, have also been developed ( Table 2).…”

Section: Fluorescence Probes and Tagging Strategiesmentioning

confidence: 99%

Understanding and Re-Engineering Nucleoprotein Machines to Cure Human Disease

et al. 2008

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SummaryThe mammalian nucleus is filled with self-organizing, nanometer-scale nucleoprotein machines that carry out DNA replication, RNA biogenesis, and DNA repair. We discuss, as a model, the nonhomologous end-joining (NHEJ) machine, which repairs DNA double-strand breaks. The NHEJ machine consists of six core polypeptides and 10-20 ancillary polypeptides. A full understanding of its design principles will require measuring the behavior of single NHEJ complexes in living cells, using a Nano Toolbox that includes bright, stable, biocompatible fluorophores, efficient protein and nucleic acid tagging strategies, and sensitive, high-resolution imaging methods. Taking inspiration from natural examples, it may be possible to adapt and redesign the NHEJ machine to precisely correct mutations responsible for common human diseases, such as K-ras in lung cancer, or human papillomavirus E6 and E7 genes in cervical and oral cancers.

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Targeting quantum dots to surface proteins in living cells with biotin ligase

Cited by 523 publications

References 42 publications

Mixed-surface, lipid-tethered quantum dots for targeting cells and tissues

Mixed-surface, lipid-tethered quantum dots for targeting cells and tissues

Nanotechnology in Cancer Diagnosis and Treatment

Understanding and Re-Engineering Nucleoprotein Machines to Cure Human Disease

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