Targeting single-walled carbon nanotubes for the treatment of breast cancer using photothermal therapy

Neves, Luís F F; Krais, John J.; Rite, Brent D. Van; Ramesh, Rajagopal; Resasco, Daniel E.; Harrison, Roger G.

doi:10.1088/0957-4484/24/37/375104

Cited by 62 publications

(59 citation statements)

References 104 publications

(180 reference statements)

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“…A5-mβG binding matched previously reported K d values of A5 alone binding to cell surface phosphatidylserine, ranging from 0.1 to 2 nM (Schutters and Reutelingsperger, 2010;Neves et al, 2013). As expected, A1-mβG exhibited higher K d values than A5-mβG.…”

Section: Discussionsupporting

confidence: 86%

Annexin-directed β-glucuronidase for the targeted treatment of solid tumors

Guillen

Ruben

Virani

et al. 2016

Protein Engineering, Design and Selection

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Enzyme prodrug therapy has the potential to remedy the lack of selectivity associated with the systemic administration of chemotherapy. However, most current systems are immunogenic and constrained to a monotherapeutic approach. We developed a new class of fusion proteins centered about the human enzyme β-glucuronidase (βG), capable of converting several innocuous prodrugs into chemotherapeutics. We targeted βG to phosphatidylserine on tumor cells, tumor vasculature and metastases via annexin A1/A5. Phosphatidylserine shows promise as a universal marker for solid tumors and allows for tumor type-independent targeting. To create fusion proteins, human annexin A1/A5 was genetically fused to the activity-enhancing 16a3 mutant of human βG, expressed in chemically defined, fed-batch suspension culture, and chromatographically purified. All fusion constructs achieved >95% purity with yields up to 740 μg/l. Fusion proteins displayed cancer selective cell-surface binding with cell line-dependent binding stability. One fusion protein in combination with the prodrug SN-38 glucuronide was as effective as the drug SN-38 on Panc-1 pancreatic cancer cells and HAAE-1 endothelial cells, and demonstrated efficacy against MCF-7 breast cancer cells. βG fusion proteins effectively enable localized combination therapy that can be tailored to each patient via prodrug selection, with promising clinical potential based on their near fully human design.

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Section: Discussionsupporting

confidence: 86%

Annexin-directed β-glucuronidase for the targeted treatment of solid tumors

Guillen

Ruben

Virani

et al. 2016

Protein Engineering, Design and Selection

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“…However, no uptake studies were performed on these cells; it would be of particular interest if the same preferential uptake observed by us was also observed with graphene nanoparticles. The efficacy of CNTs and NIR was reported for different types of cancer (6, 21–23). However, it is still unclear if this strategy can be used for glioblastoma.…”

Section: Discussionmentioning

confidence: 99%

Sequential Administration of Carbon Nanotubes and Near-Infrared Radiation for the Treatment of Gliomas

et al. 2014

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The objective was to use carbon nanotubes (CNT) coupled with near-infrared radiation (NIR) to induce hyperthermia as a novel non-ionizing radiation treatment for primary brain tumors, glioblastoma multiforme (GBM). In this study, we report the therapeutic potential of hyperthermia-induced thermal ablation using the sequential administration of carbon nanotubes (CNT) and NIR. In vitro studies were performed using glioma tumor cell lines (U251, U87, LN229, T98G). Glioma cells were incubated with CNTs for 24 h followed by exposure to NIR for 10 min. Glioma cells preferentially internalized CNTs, which upon NIR exposure, generated heat, causing necrotic cell death. There were minimal effects to normal cells, which correlate to their minimal uptake of CNTs. Furthermore, this protocol caused cell death to glioma cancer stem cells, and drug-resistant as well as drug-sensitive glioma cells. This sequential hyperthermia therapy was effective in vivo in the rodent tumor model resulting in tumor shrinkage and no recurrence after only one treatment. In conclusion, this sequence of selective CNT administration followed by NIR activation provides a new approach to the treatment of glioma, particularly drug-resistant gliomas.

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“…Neves et al functionalized SWNTs with annexin V (AV) to target the tumor vasculature of breast cancer cells. 61 In vitro studies with HAAE-1 human endothelial cells showed selective killing compared to cells treated with the laser alone. Since cells were washed thoroughly after treatment with SWNT-AV conjugates, it was clear that cell death resulted from binding of SWNT-AV to the cells.…”

Section: Carbon Nanomaterials For Thermal Ablation Of Cancer Cellsmentioning

confidence: 96%

Antitumor Therapy Using Nanomaterial-Mediated Thermolysis

Sawdon¹,

Weydemeyer²,

Peng³

2014

j biomed nanotechnol

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Nanomaterial-based systems present several novel therapeutic opportunities for cancer therapy based solely upon their unique physical and chemical properties. Despite advances in current cancer treatment, collateral damage to neighboring healthy tissues still cannot be avoided. By exploiting the strong optical and/or electromagnetic properties offered by nanomaterials, they are being employed as thermal nanoscalpels for the ablation of cancer cells. Through surface functionalization, these nanomaterials can be specifically targeted to tumorous tissue allowing for an increase in therapeutic potential and reduction in side effects. Moreover, these features provide nanomaterials with a tunable surface which can be used to modify optical, magnetic, thermal and mechanical properties. This review highlights carbon nanomaterials, nanogolds, magnetic nanoparticles and emerging hybrids applied for the thermolysis of cancer cells.

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Targeting single-walled carbon nanotubes for the treatment of breast cancer using photothermal therapy

Cited by 62 publications

References 104 publications

Annexin-directed β-glucuronidase for the targeted treatment of solid tumors

Annexin-directed β-glucuronidase for the targeted treatment of solid tumors

Sequential Administration of Carbon Nanotubes and Near-Infrared Radiation for the Treatment of Gliomas

Antitumor Therapy Using Nanomaterial-Mediated Thermolysis

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