Targeting STAT1 by myricetin and delphinidin provides efficient protection of the heart from ischemia/reperfusion‐induced injury

Scarabelli, Tiziano M.; Mariotto, Sofia; Abdel‐Azeim, Safwat; Shoji, Kazuo; Darra, Elena; Stephanou, Anastasis; Chen‐Scarabelli, Carol; Maréchal, Jean Didier; Knight, Richard; Ciampa, Anna Rosa; Saravolatz, Louis; Prati, Alessandra Carcereri de; Yuan, Zhaokan; Cavalieri, Elisabetta; Menegazzi, Marta; Latchman, David S.; Pìzza, Cosimo; Pérahia, David; Suzuki, Hisanori

doi:10.1016/j.febslet.2008.12.037

Cited by 87 publications

(62 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, recently novel protective mechanisms are arising, e.g. inhibition of signal transducer and activator of transcription 1 (STAT1) by anthocyanidin delphinidin [39], and others. Thus, modulation of heart functioning under conditions of increased oxidative stress must be ascribed to multilevel biologic activities of flavonoids, incorporating intracellular signal transduction cascades.…”

Section: Discussionmentioning

confidence: 99%

Acute Cardioprotective and Cardiotoxic Effects of Bilberry Anthocyanins in Ischemia–Reperfusion Injury: Beyond Concentration-Dependent Antioxidant Activity

et al. 2010

View full text Add to dashboard Cite

Despite being reported to reduce the risk of cardiovascular diseases, little is known about acute direct effects of bilberry anthocyanins on whole mammalian heart under ischemia-reperfusion (I-R) conditions. Bilberry anthocyanins were prepared from the ripe bilberries and analyzed using HPLC-DAD. Their antioxidant activity was evaluated by measuring the intrinsic free radical-scavenging capacity and by cellular antioxidant assay (CAA) on endothelial cells, where we quantified the intracellular capacity to inhibit the formation of peroxyl radicals. Experiments on the isolated rat hearts under I-R were carried out according to the Langendorff method. Perfusion with low concentrations of bilberry anthocyanins (0.01-1 mg/L) significantly attenuated the extent of I-R injury as evidenced by decreasing the release rate of LDH, increasing the postischemic coronary flow, and by decreasing the incidence and duration of reperfusion arrhythmias. High concentrations (5-50 mg/L) diminished cardioprotection and show cardiotoxic activity despite having their radical scavenging and intracellular antioxidant capabilities increased in a concentration-dependent manner. This study reveals the biphasic concentration-dependent bioactivity of bilberry anthocyanins under I-R, which results in strong cardioprotective activity in low concentrations and cardiotoxic activity in high concentrations.

show abstract

Section: Discussionmentioning

confidence: 99%

Acute Cardioprotective and Cardiotoxic Effects of Bilberry Anthocyanins in Ischemia–Reperfusion Injury: Beyond Concentration-Dependent Antioxidant Activity

et al. 2010

View full text Add to dashboard Cite

show abstract

“…In addition, there are reports that anthocyanins significantly inhibit TNFa-induced inflammation through monocyte chemoattractant protein-1 in human endothelium [67], suppress myocardial ischemia-reperfusion injury by delphinidin through the inhibition of signal transducers and activators of transcription 1 activity in cardiac muscle [68], and potentially inhibit the effects of C3G or its metabolite, protocatechuic acid, on atherosclerosis progression via inhibition of inflammation [69,70].…”

Section: Cvdmentioning

confidence: 99%

Dietary anthocyanin‐rich plants: Biochemical basis and recent progress in health benefits studies

Tsuda

2011

Molecular Nutrition Food Res

464

268

View full text Add to dashboard Cite

Anthocyanins are one type of flavonoid phytopigment. Although the role of anthocyanins as a functional food factor remains relatively less established than that of other flavonoids, progress in this area has been made at the molecular level in recent years. This review discusses the potential health benefits of plant-derived anthocyanin-rich foods, with a focus on the role of anthocyanins in obesity control, diabetes control, cardiovascular disease prevention, and improvement of visual and brain functions, areas that have attracted much attention. Such health benefits are not necessarily derived from the antioxidant effect of anthocyanins, but in fact are produced by currently unestablished chemical properties beyond the antioxidant capacity of the molecules. However, a better understanding of the physiological functionality of anthocyanins remains to be elucidated. It is desirable, therefore, to clarify the molecular type and composition of the anthocyanins that confer specific health benefits and to conduct further investigation into the underlying molecular mechanisms. The pharmacological actions of anthocyanins could not be fully established without knowledge on the effects of treatment of anthocyanins alone, the effects of non-anthocyanin components, and the possible interactions between anthocyanin and non-anthocyanin species.

show abstract

“…They have been shown to function as potent antioxidants and radical scavengers in vitro. Many studies have shown that increased dietary intake of flavonoid-rich foods decreases myocardial I/R injury in vivo (Hung et al, 2004;Ikizler et al, 2007;Toufektsian et al, 2008;Yamazaki et al, 2008), with many others showing protection by direct addition of flavonoids to the blood or perfusate in ex vivo isolated hearts (Amorini et al, 2003;Aneja et al, 2004;Fantinelli et al, 2005;Hirai et al, 2007;Scarabelli et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Luteolinidin Protects the Postischemic Heart through CD38 Inhibition with Preservation of NAD(P)(H)

Boslett

Hemann

Zhao

et al. 2017

J Pharmacol Exp Ther

View full text Add to dashboard Cite

We recently showed that ischemia/reperfusion (I/R) of the heart causes CD38 activation with resultant depletion of the cardiac NADP(H) pool, which is most marked in the endothelium. This NADP(H) depletion was shown to limit the production of nitric oxide by endothelial nitric oxide synthase (eNOS), which requires NADPH for nitric oxide production, resulting in greatly altered endothelial function. Therefore, intervention with CD38 inhibitors could reverse postischemic eNOS-mediated endothelial dysfunction. Here, we evaluated the potency of the CD38 inhibitor luteolinidin, an anthocyanidin, at blocking CD38 activity and preserving endothelial and myocardial function in the postischemic heart. Initially, we characterized luteolinidin as a CD38 inhibitor in vitro to determine its potency and mechanism of inhibition. We then tested luteolinidin in the ex vivo isolated heart model, where we determined luteolinidin uptake with aqueous and liposomal delivery methods. Optimal delivery methods were then further tested to determine the effect of luteolinidin on postischemic NAD(P)(H) and tetrahydrobiopterin levels. Finally, through nitric oxide synthase-dependent coronary flow and left ventricular functional measurements, we evaluated the efficacy of luteolinidin to protect vascular and contractile function, respectively, after I/R. With enhanced postischemic preservation of NADPH and tetrahydrobiopterin, there was a dose-dependent effect of luteolinidin on increasing recovery of endothelium-dependent vasodilatory function, as well as enhancing the recovery of left ventricular contractile function with increased myocardial salvage. Thus, luteolinidin is a potent CD38 inhibitor that protects the heart against I/R injury with preservation of eNOS function and prevention of endothelial dysfunction.

show abstract

Targeting STAT1 by myricetin and delphinidin provides efficient protection of the heart from ischemia/reperfusion‐induced injury

Cited by 87 publications

References 33 publications

Acute Cardioprotective and Cardiotoxic Effects of Bilberry Anthocyanins in Ischemia–Reperfusion Injury: Beyond Concentration-Dependent Antioxidant Activity

Acute Cardioprotective and Cardiotoxic Effects of Bilberry Anthocyanins in Ischemia–Reperfusion Injury: Beyond Concentration-Dependent Antioxidant Activity

Dietary anthocyanin‐rich plants: Biochemical basis and recent progress in health benefits studies

Luteolinidin Protects the Postischemic Heart through CD38 Inhibition with Preservation of NAD(P)(H)

Contact Info

Product

Resources

About