Targeting steroid receptor RNA activator (SRA), a long non-coding RNA, enhances melanogenesis through activation of TRP1 and inhibition of p38 phosphorylation

Ho, Ji Chen; Lee, Chih Hung; Hong, Chien Hui

doi:10.1371/journal.pone.0237577

Cited by 10 publications

(5 citation statements)

References 35 publications

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“…By employing genome-wide analysis, Ren et al (2016) identified numerous lncRNAs associated with skin pigmentation in goats. Furthermore, evidence shows that lncRNAs can regulate melanogenesis via epigenetic regulation, while miRNAs can directly target many melanogenesis-related genes (Ho et al, 2020;review by Zhou et al, 2021). In aquaculture species, numerous significant differentially expressed lncRNAs (DElncRNAs) and miRNAs (DEmiRNAs) have been identified or further investigated by using different skin colors or other pigmented tissues in numerous species, such as Koi carp (Cyprinus carpio L.) (Luo et al, 2018(Luo et al, , 2019Dong et al, 2020;Yin et al, 2020), Pacific oyster (Crassostrea gigas) (Feng et al, 2018;Li Z. et al, 2021), red tilapia (Oreochromis sp.…”

Section: Introductionmentioning

confidence: 99%

Identification of Potential Blind-Side Hypermelanosis-Related lncRNA–miRNA–mRNA Regulatory Network in a Flatfish Species, Chinese Tongue Sole (Cynoglossus semilaevis)

Peng

et al. 2022

Front. Genet.

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Blind-side hypermelanosis has emerged as a major concern in commercial rearing environments of the flatfish aquaculture industry. To date, the underlying molecular mechanisms are not well understood. To fill this gap, in this study, whole transcriptomic sequencing and analyses were performed using normal skins and hypermelanic skins of the blind side of Chinese tongue sole (Cynoglossus semilaevis). Differentially expressed long non-coding RNAs (DElncRNAs), miRNAs (DEmiRNAs), and differentially expressed genes as well as their competing endogenous RNA (ceRNA) networks were identified. A total of 34 DElncRNAs, 226 DEmiRNAs, and 610 DEGs were identified. Finally, lncRNA–miRNA–mRNA regulatory networks (involving 29 DElncRNAs, 106 DEmiRNAs, and 162 DEGs) associated with blind-side hypermelanosis were constructed. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of 162 DEGs in ceRNA networks identified DEGs (e.g., oca2, mc1r, and ihhb) in pigmentation-related biological processes and DEGs (e.g., ca4, glul, and fut9) in nitrogen metabolism, glycosphingolipid biosynthesis, and folate biosynthesis pathways, as well as their corresponding DElncRNAs and DEmiRNAs to potentially play key regulatory roles in blind-side hypermelanosis. In conclusion, this is the first study on the ceRNA regulatory network associated with blind-side hypermelanosis in flatfish. These new findings expand the spectrum of non-coding regulatory mechanisms underpinning blind-side hypermelanosis, which facilitates the further exploration of molecular regulatory mechanisms of malpigmentation in flatfish.

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Section: Introductionmentioning

confidence: 99%

Identification of Potential Blind-Side Hypermelanosis-Related lncRNA–miRNA–mRNA Regulatory Network in a Flatfish Species, Chinese Tongue Sole (Cynoglossus semilaevis)

Peng

et al. 2022

Front. Genet.

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“…Although some lncRNAs have been proven to participate in the regulation of pigmentation process, such as H19, UCA1, SPRIGHTLY, TUG1, TCONS_00049140, ENST00000606533, SRA, etc. [14][15][16][17][18][19][20], their number is small, and there are still many lncRNAs related to pigmentation waiting to be discovered. In this study, we detected the lncRNA profiles in black and white sheep skin and obtained 4,904 lncRNAs, among which 2,686 novel lncRNAs were identified.…”

Section: Discussionmentioning

confidence: 99%

“…ENST00000606533-miR-1291-TYR promotes melanogenesis [17], whereas SRA, UCA1 and TUG1 may negatively regulate melanogenesis in melanocytes [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive analysis of lncRNAs modified by m6A methylation in sheep skin

Meng,

Li,

Zhao

2024

Anim Biosci

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Objective: N6-methyladenosine (m 6 A) is the most prevalent methylation of mRNA and plays crucial roles in various physiological processes, including pigmentation. Yet, the regulatory mechanisms, including long noncoding RNAs (lncRNAs) m 6 A methylation contributing to pigmentation in sheep skin remains unclear. The purpose of this study was to identify potential lncRNAs and the m 6 A methylation of lncRNAs associated with pigmentation.Methods: RNA-seq and MeRIP-seq were performed to study the expression of lncRNAs and the m 6 A methylation of lncRNAs in black and white sheep skin. Furthermore, quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the consistency with the RNA-seq and MeRIP-seq data.Results: 168 differentially expressed lncRNAs were detected between the two sheep skin colors. The differentially expressed lncRNAs enriched in the pathway of ECM-receptor interaction, Rap1 signaling pathway, and Non-homologous end-joining may play essential roles in pigmentation. We identified 577 m 6 A peaks and 617 m 6 A peaks in black and white sheep skin, respectively, among which 20 m 6 A peaks showed significant differences. The enriched motif in sheep skin was "GGACU", which aligned with the consensus motif "RRACH" (R=A or G, H=A, C or U). Differently methylated lncRNAs enriched in PI3K-Akt signaling pathway and Wnt signaling pathway might participate in skin pigmentation. ENSOARG00020015168 was the unique lncRNA with high expression and methylation (Hyper-Up) in black sheep shin. A lncRNA-mRNA network was constructed, with pigmentation-related genes, such as PSEN2, CCND3, COL2A1, and ERCC3. Conclusion:The m 6 A modifications of lncRNAs in black and white colored sheep skin were analyzed comprehensively, providing new candidates for the regulation of pigmentation.

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“…For intracellular experiments, the human melanocyte line that depends on the activation of TRY pathway is a key element in efficiently evaluating the anti-melanin synthesis effect of WFP. The A375 cell line, a metastatic cell line of the human amelanotic melanoma, has been widely used as a reliable model for screening anti-melanin agents [ 41 – 43 ], which is dependent on TRY pathway activation [ 44 , 45 ]. Firstly, thecytotoxic effect of WFPin A375 cells should be evaluated, and eliminate the influence caused by the decease of melanin synthesis due to cell numbers decline.…”

Section: Discussionmentioning

confidence: 99%

Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells

Liao

Zhang

et al. 2022

BMC Complement Med Ther

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Background Polysaccharides from wampee have been reported to process various biological activities, while the relationship between structure and bioactivities has been barely addressed. Pectin, an abundant water-soluble polysaccharide in wampee, showed significant antioxidant activity, which was associated with the anti-melanogenic activity. Therefore, this study investigated the physicochemical characteristics and the anti-melanogenesis effect of pectin extracted from wampee fruit in A375 cells. Methods The physicochemical characterization of pectin from wampee fruit was investigated by gel chromatography (GCP), FT-IR spectroscopy, and NMR spectroscopy methods. The anti-melanogenesis effects and mechanism were evaluated by mushroom tyrosine enzyme and human melanin cell model in vitro. Results The results showed that a molecular weight of 5.271 × 105 Da wampee fruit pectin (WFP) were mainly composed of mannose (Man), ribose (Rib), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galacturonic acid (Gal A), galactose (Gal), and arabinose (Ara), which linked with →4)-β-D-Galp-(1 → units. The current study revealed that WFP could significantly suppress mushroom TRY activity in vitro. Furtherly, WFP significantly reduced intracellular and extracellular melanin formation in A375 melanoma cells depending on the presence of alpha-melanocyte stimulating hormone (α-MSH). TRY activity was only inhibited in α-MSH treated A375 cells. Western blot analysis demonstrated that WFP reverse α-MSH induced melanogenesis in A375 melanoma cells, including in down-regulated TRY, TYRP-1, TYRP-2, MITF and CREB expressions. Conclusion These results indicated that WFP could inhibit α-MSH induced melanogenesis in A375 melanoma cells via α-MSH/TRY pathway. In conclusion, these data provided a new perspective to annotate WFP anti-melanogenesis activity mechanism.

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Targeting steroid receptor RNA activator (SRA), a long non-coding RNA, enhances melanogenesis through activation of TRP1 and inhibition of p38 phosphorylation

Cited by 10 publications

References 35 publications

Identification of Potential Blind-Side Hypermelanosis-Related lncRNA–miRNA–mRNA Regulatory Network in a Flatfish Species, Chinese Tongue Sole (Cynoglossus semilaevis)

Identification of Potential Blind-Side Hypermelanosis-Related lncRNA–miRNA–mRNA Regulatory Network in a Flatfish Species, Chinese Tongue Sole (Cynoglossus semilaevis)

Comprehensive analysis of lncRNAs modified by m6A methylation in sheep skin

Anti-melanogenesis effect from Wampee fruit pectin via α-MSH/TRY pathway in A375 cells

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