Targeting symbiosis-related insect genes by RNAi in the pea aphid- Buchnera symbiosis

Chung, Seung Ho; Jing, Xin; Luo, Yuan; Douglas, Angela E.

doi:10.1016/j.ibmb.2018.02.004

Cited by 77 publications

(77 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Another important factor in RNAi efficiency is the stability of the dsRNA in extracellular environments in the body, such as the gut lumen and the hemolymph. However, no studies have been published investigating the potential for degradation of dsRNA by certain nucleases such as DNA/RNA non‐specific nucleases, which have been implicated in affecting RNAi efficiency in insects . In mites, dsRNA is normally ingested, delivered by immersion or injected, and each of these dsRNA delivery methods has led to successful gene silencing at the mRNA level, implying that dsRNA is not completely degraded by the different delivery methods.…”

Section: Possible Factors That Enhance Systemic Movement Of Rna In MImentioning

confidence: 99%

“…However, no studies have been published investigating the potential for degradation of dsRNA by certain nucleases such as DNA/RNA non-specific nucleases, which have been implicated in affecting RNAi efficiency in insects. 54,55 In mites, dsRNA is normally ingested, delivered by immersion or injected, and each of these dsRNA delivery methods has led to successful gene silencing at the mRNA level, implying that dsRNA is not completely degraded by the different delivery methods. However, they might still have an effect on RNAi efficiency and could be an important mechanism by which resistance to RNAi-based pest control strategies could occur.…”

Section: Possible Factors That Enhance Systemic Movement Of Rnai In Mmentioning

confidence: 99%

See 1 more Smart Citation

Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Niu

Shen

Christiaens

et al. 2018

Pest Management Science

View full text Add to dashboard Cite

Mites comprise a group of key agricultural pests on a wide range of crops. They cause harm through feeding on the plant and transferring dangerous pathogens, and the rapid evolution of pesticide resistance in mites highlights the need for novel control methods. Currently, RNA interference (RNAi) shows great potential for insect pest control. Here, we review the literature regarding RNAi in mite pests. We discuss different target genes and RNAi efficiency in various mite species, a promising Varroa control program using RNAi, the synergy of RNAi with plant defense mechanisms and microorganisms, and current understanding of systemic movement of double-stranded RNA (dsRNA). On the basis of this evidence, we can conclude that there is clear potential for application of RNAi-based mite control, but further research on several aspects of RNAi in mites is needed, including: (i) the factors influencing RNAi efficiency, (ii) the mechanism of environmental RNAi and cross-kingdom dsRNA trafficking, (iii) the mechanism of possible systemic and parental RNAi, and (iv) non-target effects, specifically in predatory mites, which should be considered during RNAi target selection. © 2018 Society of Chemical Industry.

show abstract

Section: Possible Factors That Enhance Systemic Movement Of Rna In MImentioning

confidence: 99%

Section: Possible Factors That Enhance Systemic Movement Of Rnai In Mmentioning

confidence: 99%

Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Niu

Shen

Christiaens

et al. 2018

Pest Management Science

View full text Add to dashboard Cite

show abstract

“…These authors concluded that these two host enzymes could clean PG products derived from Buchnera to minimize in this manner immune recognition. Intriguingly, amiD and ldcA1 are overexpressed in the bacteriocytes (Chung et al, ). As some endosymbionts are essential for insect development, controlling endosymbiont fitness by altering expression of host genes encoding PG enzymes is currently intensively investigated to control insect pests.…”

Section: Does the Host Participate In Pg Metabolism Of Intracellular mentioning

confidence: 99%

“…A completely new view has recently put into scene by two studies involving the insect endosymbionts Buchnera spp. (Chung, Jing, Luo, & Douglas, 2018) and Candidatus Moranella. This latter endosymbiont lives inside another bacterium, Candidatus Tremblaya, which also lives inside specialized insect cells -bacteriocytes-of mealybugs (Bublitz et al, 2019).…”

Section: Doe S the Hos T Parti Cipate In P G Me Tabolis M Of Intr Amentioning

confidence: 99%

“…Intriguingly, amiD and ldcA1 are overexpressed in the bacteriocytes (Chung et al, 2018). As some endosymbionts are essential for insect development, controlling endosymbiont fitness by altering expression of host genes encoding PG enzymes is currently intensively investigated to control insect pests.…”

Section: Doe S the Hos T Parti Cipate In P G Me Tabolis M Of Intr Amentioning

confidence: 99%

See 1 more Smart Citation

Building peptidoglycan inside eukaryotic cells: A view from symbiotic and pathogenic bacteria

Portillo

2020

Molecular Microbiology

View full text Add to dashboard Cite

The peptidoglycan (PG), as the exoskeleton of most prokaryotes, maintains a defined shape and ensures cell integrity against the high internal turgor pressure. These important roles have attracted researchers to target PG metabolism in order to control bacterial infections. Most studies, however, have been performed in bacteria grown under laboratory conditions, leading to only a partial view on how the PG is synthetized in natural environments. As a case in point, PG metabolism and its regulation remain poorly understood in symbiotic and pathogenic bacteria living inside eukaryotic cells. This review focuses on the PG metabolism of intracellular bacteria, emphasizing the necessity of more in vivo studies involving the analysis of enzymes produced in the intracellular niche and the isolation of PG from bacteria residing within eukaryotic cells. The review also points to persistent infections caused by some intracellular bacterial pathogens and the extent at which the PG could contribute to establish such physiological state. Based on recent evidences, I speculate on the idea that certain structural features of the PG may facilitate attenuation of intracellular growth. Lastly, I discuss recent findings in endosymbionts supporting a cooperation between host and bacterial enzymes to assemble a mature PG.

show abstract

Identification and functional analysis of dsRNases in spotted‐wing drosophila, Drosophila suzukii

Yoon

Ahn

Flinn

et al. 2021

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

RNAi efficiency in insects is different from species to species; some species in Coleoptera are relatively more amenable to RNA interference (RNAi) than other species.One of the major factors is the presence of dsRNAdegrading enzymes, called dsRNases, in saliva, gut, or hemolymph in insects, which degrade the double-stranded RNA (dsRNA) introduced, resulting in the low efficacy of RNAi. In this study, we report a dsRNA-degrading activity in the gut homogenates from the spotted-wing drosophila, Drosophila suzukii, by ex vivo assay. Then, we identified two Drosophila suzukii dsRNase genes, named DrosudsRNase1 and DrosudsRNase2. In silico analysis shows that the gene structures are similar to dsRNases found in other insects.When dsRNases expressed in Sf9 cells were compared for their dsRNA degrading activities, dsRNase1 was more vital than dsRNase2. Both dsRNases were expressed highly and exclusively in the gut compared to the rest of body. Also, they were highly expressed during larval and adult stages but not in embryonic and pupal stages, suggesting the dsRNases protect foreign RNA molecules received during the feeding periods. DsRNase1 was expressed at a higher level in adults, whereas dsRNase2 showed more expression in early larvae. Our study on the tissue and development-This article has been contributed to by US Government employees and their work is in the public domain in the USA.

show abstract

Targeting symbiosis-related insect genes by RNAi in the pea aphid- Buchnera symbiosis

Cited by 77 publications

References 66 publications

Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Beyond insects: current status and achievements of RNA interference in mite pests and future perspectives

Building peptidoglycan inside eukaryotic cells: A view from symbiotic and pathogenic bacteria

Identification and functional analysis of dsRNases in spotted‐wing drosophila, Drosophila suzukii

Contact Info

Product

Resources

About