Targeting the stem cell niche: role of collagen XVII in skin aging and wound repair

Liu, Yangdan; Ho, Chia-Kang; Wen, Dongsheng; Sun, Jian; Lu, Haojie; Gao, Ya; Li, Qingfeng; Zhang, Yifan

doi:10.7150/thno.78016

Cited by 13 publications

(8 citation statements)

References 82 publications

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“…The transcriptome data were further combined with literature analysis to identify the representative genes representing aging. Twenty genes were selected, namely CCNB1, CCNB2, CDK1, KDR, FOXO3 [ 21 ], ITGA11 [ 22 ], CXCL12 [ 23 ], TSG101, DUSP22 [ 24 ], COL17A1 [ 25 ], LRRN3 [ 26 ], CRIP2 [ 26 ], VCP [ 27 ], E2F2 [ 28 ], GTSE1 [ 29 ], PLK4 [ 30 ], CDC25C [ 29 ], WNT16 [ 31 ], PCNA [ 32 ], RRM2 [ 33 ]. Heat maps were generated by plotting the expression levels of these senescence-associated gene population in six groups of samples (Fig.…”

Section: Resultsmentioning

confidence: 99%

Efficient expansion and delayed senescence of hUC-MSCs by microcarrier–bioreactor system

Wang,

Ouyang,

Chen

et al. 2023

Stem Cell Res Ther

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Background Human umbilical cord mesenchymal stem cells (hUC-MSCs) are widely used in cell therapy due to their robust immunomodulatory and tissue regenerative capabilities. Currently, the predominant method for obtaining hUC-MSCs for clinical use is through planar culture expansion, which presents several limitations. Specifically, continuous cell passaging can lead to cellular aging, susceptibility to contamination, and an absence of process monitoring and control, among other limitations. To overcome these challenges, the technology of microcarrier–bioreactor culture was developed with the aim of ensuring the therapeutic efficacy of cells while enabling large-scale expansion to meet clinical requirements. However, there is still a knowledge gap regarding the comparison of biological differences in cells obtained through different culture methods. Methods We developed a culture process for hUC-MSCs using self-made microcarrier and stirred bioreactor. This study systematically compares the biological properties of hUC-MSCs amplified through planar culture and microcarrier–bioreactor systems. Additionally, RNA-seq was employed to compare the differences in gene expression profiles between the two cultures, facilitating the identification of pathways and genes associated with cell aging. Results The findings revealed that hUC-MSCs expanded on microcarriers exhibited a lower degree of cellular aging compared to those expanded through planar culture. Additionally, these microcarrier-expanded hUC-MSCs showed an enhanced proliferation capacity and a reduced number of cells in the cell cycle retardation period. Moreover, bioreactor-cultured cells differ significantly from planar cultures in the expression of genes associated with the cytoskeleton and extracellular matrix. Conclusions The results of this study demonstrate that our microcarrier–bioreactor culture method enhances the proliferation efficiency of hUC-MSCs. Moreover, this culture method exhibits the potential to delay the process of cell aging while preserving the essential stem cell properties of hUC-MSCs.

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Section: Resultsmentioning

confidence: 99%

Efficient expansion and delayed senescence of hUC-MSCs by microcarrier–bioreactor system

Wang,

Ouyang,

Chen

et al. 2023

Stem Cell Res Ther

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“…Skin aging is promoted by both intrinsic factors (resulting from physiological processes) and extrinsic factors (such as exposure to ultraviolet radiation and pollutants) ( Wang and Dreesen, 2018 ). In aging skin, a functional decline occurs in stem cells, such as epidermal stem cells (ESCs), hair follicle stem cells (HFSCs), and melanocyte stem cells, leading to skin thinning, vulnerability, and impaired wound healing ( Ashcroft et al, 2002 ; Hsu et al, 2014 ; Liu et al, 2022 ). Various differentiated cells, including keratinocytes, fibroblasts, immune cells, and melanocytes, also undergo functional decline, resulting in the manifestation of the characteristic features of aging skin ( Wang and Dreesen, 2018 ; Chambers and Vukmanovic-Stejic, 2020 ).…”

Section: Role Of Cellular Senescence In Wound Healing In Aged Skinmentioning

confidence: 99%

Cellular senescence and wound healing in aged and diabetic skin

Kita,

Yamamoto,

Saito

et al. 2024

Front. Physiol.

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Cellular senescence is a biological mechanism that prevents abnormal cell proliferation during tissue repair, and it is often accompanied by the secretion of various factors, such as cytokines and chemokines, known as the senescence-associated secretory phenotype (SASP). SASP-mediated cell-to-cell communication promotes tissue repair, regeneration, and development. However, senescent cells can accumulate abnormally at injury sites, leading to excessive inflammation, tissue dysfunction, and intractable wounds. The effects of cellular senescence on skin wound healing can be both beneficial and detrimental, depending on the condition. Here, we reviewed the functional differences in cellular senescence that emerge during wound healing, chronic inflammation, and skin aging. We also review the latest mechanisms of wound healing in the epidermis, dermis, and subcutaneous fat, with a focus on cellular senescence, chronic inflammation, and tissue regeneration. Finally, we discuss the potential clinical applications of promoting and inhibiting cellular senescence to maximize benefits and minimize detrimental effects.

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“…In the context of skin health, the decline in stem cell populations is closely associated with skin atrophy, fragility, and changes in pigmentation [ 79 ]. While the self-renewal capacity of stem cells tends to decrease with skin aging, it is observed to increase during wound repair [ 80 ]. Amniotic membrane stem cells (AMSCs), adipose-derived stem cells (ADSCs), and human umbilical cord mesenchymal stem cells (hUC-MSCs) are all being explored for their potential in skin rejuvenation due to their regenerative properties [ 81 ].…”

Section: Reviewmentioning

confidence: 99%

Clinically Actionable Topical Strategies for Addressing the Hallmarks of Skin Aging: A Primer for Aesthetic Medicine Practitioners

Minoretti,

Emanuele

2024

Cureus

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In this narrative review, we sought to provide a comprehensive overview of the mechanisms underlying cutaneous senescence, framed by the twelve traditional hallmarks of aging. These include genomic instability, telomere attrition, epigenetic alterations, loss of proteostasis, impaired macroautophagy, deregulated nutrient sensing, mitochondrial dysfunction, cellular senescence, stem cell exhaustion, altered intercellular communication, chronic inflammation, and dysbiosis. We also examined how topical interventions targeting these hallmarks can be integrated with conventional aesthetic medicine techniques to enhance skin rejuvenation. The potential of combining targeted topical therapies against the aging hallmarks with minimally invasive procedures represents a significant advancement in aesthetic medicine, offering personalized and effective strategies to combat skin aging. The reviewed evidence paves the way for future advancements and underscores the transformative potential of integrating scientifically validated interventions targeted against aging hallmarks into traditional aesthetic practices.

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Targeting the stem cell niche: role of collagen XVII in skin aging and wound repair

Cited by 13 publications

References 82 publications

Efficient expansion and delayed senescence of hUC-MSCs by microcarrier–bioreactor system

Efficient expansion and delayed senescence of hUC-MSCs by microcarrier–bioreactor system

Cellular senescence and wound healing in aged and diabetic skin

Clinically Actionable Topical Strategies for Addressing the Hallmarks of Skin Aging: A Primer for Aesthetic Medicine Practitioners

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