Tartrate/tripolyphosphate as co-crosslinker for water soluble chitosan used in protein antigens encapsulation

Srivastava, G.; Walke, Shilratna; Dhavale, Dilip D.; Gade, W. N.; Doshi, Jignesh; Kumar, Rakesh; Ravetkar, Satish; Doshi, Pooja

doi:10.1016/j.ijbiomac.2016.05.099

Cited by 21 publications

(7 citation statements)

References 90 publications

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“…Our findings support the notion that the positively charged amine groups of CS are mainly responsible for the carrier’s bioadhesive propensity [40]. This phenomenon occurs specifically at the molecular level, through electrostatic interactions between the positively charged amino groups of CS and the negatively charged sulphonic acid resides in the mucin, bringing about rheological synergism [41,42,43]. Hence, this system has the potential to pass through the mucosal layer, enhancing its attractiveness as a drug delivery system.…”

Section: Discussionsupporting

confidence: 85%

The Synergism of Platinum-Gold Bimetallic Nanoconjugates Enhances 5-Fluorouracil Delivery In Vitro

Maney

Singh

2019

Pharmaceutics

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Nanoparticle application has significantly impacted the field of medicine. The need to develop novel drugs with higher therapeutic potential has stimulated the development of innovative delivery strategies to mitigate the potent side effects associated with known chemotherapeutic drugs. This paper describes the synthesis of platinum-gold bimetallic nanoparticles (PtAuBNps), their functionalisation with chitosan, and entrapment of the anticancer drug 5-fluorouracil (5-FU). All PtAuBNps and their drug nanocomposites were physico-chemically characterised, displaying desirable properties with regards to shape, size (<120 nm) and colloidal stability. 5-FU binding and loading capacities in PtAuBNps were found to be 90.17% and 22.56%, respectively. In vitro cytotoxicity profiles determined using the MTT and SRB assays reflected up to 65% cell death in the MCF-7, HepG2 and Caco-2 cell lines. These nanocomposites exhibited excellent physiochemical attributes, high specificity towards cancer cells, with a pH-sensitive drug release in a simulated acidic tumour microenvironment through zero-order release kinetics. In addition, they possessed the potential to traverse the mucosal lining facilitating oral drug administration. Overall, 5-FU encapsulation improved the bioavailability of the drug in cancer cells, with the promise of enhancing its therapeutic effect, biocompatibility and safety. These positive results highlight PtAuBNps as promising in vitro delivery systems and merits future in vivo research.

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Section: Discussionsupporting

confidence: 85%

The Synergism of Platinum-Gold Bimetallic Nanoconjugates Enhances 5-Fluorouracil Delivery In Vitro

Maney

Singh

2019

Pharmaceutics

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“…In SGF, 50% of the loaded drug was released in almost 24 h, while this took place in PBS in less than 10 h. Moreover, as regards C max , 81% in PBS and 78% in SGF was reached in less than 2 days and after 3 days, respectively. As described in both our previous and other studies (Di Martino et al 2016 ; Di Martino et al 2015 ; Srivastava et al 2016 ; Soares et al 2016 ), such release is strictly bound with the swelling index; in polyelectrolytes, swelling is directly influenced by the pH and ionic strength of the surrounding environment. Furthermore, the presence of hydrophobic side chains affects the swelling ability and subsequently in the release of drugs, e.g., TMZ, which are mildly soluble in water (5 mg/mL).…”

Section: Resultssupporting

confidence: 68%

Enhancement of temozolomide stability by loading in chitosan-carboxylated polylactide-based nanoparticles

et al. 2017

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In the presented work, amphiphilic nanoparticles based on chitosan and carboxy-enriched polylactic acid have been prepared to improve the stability of the pro-drug temozolomide in physiological media by encapsulation. The carrier, with a diameter in the range of 150–180 nm, was able to accommodate up to 800 μg of temozolomide per mg of polymer. The obtained formulation showed good stability in physiological condition and preparation media up to 1 month. Temozolomide loaded inside the carrier exhibited greater stability than the free drug, in particular in simulated physiological solution at pH 7.4 where the hydrolysis in the inactive metabolite was clearly delayed. CS-SPLA nanoparticles demonstrated a pH-dependent TMZ release kinetics with the opportunity to increase or decrease the rate. Mass spectroscopy, UV-Vis analysis, and in vitro cell tests confirmed the improvement in temozolomide stability and effectiveness when loaded into the polymeric carrier, in comparison with the free drug.Electronic supplementary materialThe online version of this article (doi:10.1007/s11051-017-3756-3) contains supplementary material, which is available to authorized users.

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“…Chitosan is actually insoluble in water at physiological pH, which limits it from being widely used in many biomedical applications as tissue engineering scaffolds, drug carriers for controlled release, and genetic materials [24]. glucosamine units (1082 cm -1 , 1028 cm -1 , and 897 cm -1 ), similar to those reported in the literature [25].…”

Section: Synthesis Of Mcs and Msf Micro/nanoparticlessupporting

confidence: 60%

Photopolymerized maleilated chitosan/methacrylated silk fibroin micro/nanocomposite hydrogels as potential scaffolds for cartilage tissue engineering

Zhou

Liang

Zhao

et al. 2018

International Journal of Biological Macromolecules

103

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Hydrogels composed of natural materials exhibit great application potential in artificial scaffolds for cartilage repair as they can resemble the extracellular matrices of cartilage tissues comprised of various glycosaminoglycan and collagen. Herein, the natural polymers with vinyl groups, i.e. maleilated chitosan (MCS) and methacrylated silk fibroin (MSF) micro/nanoparticles, were firstly synthesized. The chemical structures of MCS and MSF micro/nanoparticles were investigated using Fourier transform infrared (FTIR) spectroscopy, proton nuclear magnetic resonance (H NMR) spectroscopy, and X-ray photoelectron spectroscopy (XPS). Then MCS/MSF micro/nanocomposite hydrogels were prepared by the photocrosslinking of MCS and MSF micro/nanoparticles in aqueous solutions in the presence of the photoinitiator Darocur 2959 under UV light irradiation. A series of properties of the MCS/MSF micro/nanocomposite hydrogels including rheological property, equilibrium swelling, sol content, compressive modulus, and morphology were examined. The results showed that these behaviors could be tunable via the control of MSF content. When the MSF content was 0.1%, the hydrogel had the compressive modulus of 0.32±0.07MPa, which was in the range of that of articular cartilage. The in vitro cytotoxic evaluation and cell culture of the micro/nanocomposite hydrogels in combination with mouse articular chondrocytes were also investigated. The results demonstrated that the micro/nanocomposite hydrogels with TGF-β1 was biocompatible to mouse articular chondrocytes and could support cells attachment well, indicating their potential as tissue engineering scaffolds for cartilage repair.

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Tartrate/tripolyphosphate as co-crosslinker for water soluble chitosan used in protein antigens encapsulation

Cited by 21 publications

References 90 publications

The Synergism of Platinum-Gold Bimetallic Nanoconjugates Enhances 5-Fluorouracil Delivery In Vitro

The Synergism of Platinum-Gold Bimetallic Nanoconjugates Enhances 5-Fluorouracil Delivery In Vitro

Enhancement of temozolomide stability by loading in chitosan-carboxylated polylactide-based nanoparticles

Photopolymerized maleilated chitosan/methacrylated silk fibroin micro/nanocomposite hydrogels as potential scaffolds for cartilage tissue engineering

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