TaSnRK2.9, a Sucrose Non-fermenting 1-Related Protein Kinase Gene, Positively Regulates Plant Response to Drought and Salt Stress in Transgenic Tobacco

Feng, Jialu; Wang, Lianzhe; Wu, Yanan; Luo, Qingchen; Zhang, Yang; Qiu, Ding; Han, Jiapeng; Su, Peipei; Xiong, Zhiyong; Chang, Junli; Yang, Guangxiao; He, Guoqing

doi:10.3389/fpls.2018.02003

Cited by 53 publications

(38 citation statements)

References 62 publications

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“…The expression of GhSnRK2.6 was shown to be up-regulated under salt conditions, and further demonstrated that GhSnRK2.6 improved salt tolerance in transgenic upland cotton and A. thaliana [48]. In addition, TaSnRK2.9 positively regulates the response of transgenic tobacco plants to drought and salt stress [30]. The EgrSnRK2.6 clusters with these gens, but was up-regulated to two-fold with increasing salt concentrations.…”

Section: Discussionmentioning

confidence: 99%

“…PtSnRK2.5 and PtSnRK2.7 positively regulate plant responses to salt stress [28]. The overexpression of TaSnRK2.3 , TaSnRK2.4 , TaSnRK2.7 and TaSnRK2.8 in A. thaliana was reported to enhance plants tolerance to salt and other stresses [29,30]. Recent studies have shown that TaSnRK2.9 improved salt and drought stress tolerance through detoxification of (reactive oxygen species) ROS [30].…”

Section: Introductionmentioning

confidence: 99%

“…The overexpression of TaSnRK2.3 , TaSnRK2.4 , TaSnRK2.7 and TaSnRK2.8 in A. thaliana was reported to enhance plants tolerance to salt and other stresses [29,30]. Recent studies have shown that TaSnRK2.9 improved salt and drought stress tolerance through detoxification of (reactive oxygen species) ROS [30]. Additionally, overexpression of GhCIPK6 significantly increased the tolerance of transgenic A.thaliana to salt, drought and ABA stress [31].…”

Section: Introductionmentioning

confidence: 99%

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Comprehensive Analysis of SnRK Gene Family and their Responses to Salt Stress in Eucalyptus grandis

Wang

Yan

Zhenfei

et al. 2019

IJMS

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The sucrose non-fermentation-related protein kinase (SnRK) is a kind of Ser/Thr protein kinase, which plays a crucial role in plant stress response by phosphorylating the target protein to regulate the interconnection of various signaling pathways. However, little is known about the SnRK family in Eucalyptus grandis. Thirty-four putative SnRK sequences were identified in E. grandis and divided into three subgroups (SnRK1, SnRK2 and SnRK3) based on phylogenetic analysis and the type of domain. Chromosome localization showed that SnRK family members are unevenly distributed in the remaining 10 chromosomes, with the notable exception of chromosome 11. Gene structure analysis reveal that 10 of the 24 SnRK3 genes contained no introns. Moreover, conserved motif analyses showed that SnRK sequences belonged to the same subgroup that contained the same motif type of motif. The Ka/Ks ratio of 17 paralogues suggested that the EgrSnRK gene family underwent a purifying selection. The upstream region of EgrSnRK genes enriched with different type and numbers of cis-elements indicated that EgrSnRK genes are likely to play a role in the response to diverse stresses. Quantitative real-time PCR showed that the majority of the SnRK genes were induced by salt treatment. Genome-wide analyses and expression pattern analyses provided further understanding on the function of the SnRK family in the stress response to different environmental salt concentrations.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comprehensive Analysis of SnRK Gene Family and their Responses to Salt Stress in Eucalyptus grandis

Wang

Yan

Zhenfei

et al. 2019

IJMS

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“…The activities of POD and SOD, H 2 O 2 content, and GSH level in soybean hairy roots were measured as described previously [63,64]. In brief, total proteins were extracted using the vegetable protein extraction kit (KeyGene BioTECH, Nanjing, China) and quantified using a BCA protein assay kit (KeyGene BioTECH).…”

Section: Methodsmentioning

confidence: 99%

Overexpression of Peroxidase Gene GsPRX9 Confers Salt Tolerance in Soybean

Jin

Sun

Zhao

et al. 2019

IJMS

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Peroxidases play prominent roles in antioxidant responses and stress tolerance in plants; however, their functions in soybean tolerance to salt stress remain unclear. Here, we investigated the role of a peroxidase gene from the wild soybean (Glycine soja), GsPRX9, in soybean tolerance to salt stress. GsPRX9 gene expression was induced by salt treatment in the roots of both salt-tolerant and -sensitive soybean varieties, and its relative expression level in the roots of salt-tolerant soybean varieties showed a significantly higher increase than in salt-sensitive varieties after NaCl treatment, suggesting its possible role in soybean response to salt stress. GsPRX9-overexpressing yeast (strains of INVSc1 and G19) grew better than the control under salt and H2O2 stress, and GsPRX9-overexpressing soybean composite plants showed higher shoot fresh weight and leaf relative water content than control plants after NaCl treatment. Moreover, the GsPRX9-overexpressing soybean hairy roots had higher root fresh weight, primary root length, activities of peroxidase and superoxide dismutase, and glutathione level, but lower H2O2 content than those in control roots under salt stress. These findings suggest that the overexpression of the GsPRX9 gene enhanced the salt tolerance and antioxidant response in soybean. This study would provide new insights into the role of peroxidase in plant tolerance to salt stress.

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“…Several other studies have shown that overexpression of different drought responsive genes confers greater drought tolerance in transgenic wheat including the genes AtHDG11 (Li et al, 2016), TaCIPK23 (Cui et al, 2018), TaBZR2 (Cui et al, 2019), TaWRKY2 (Gao et al, 2018), TaWRKY1, and TaWRKY33 (He et al, 2016), and TaPYL4 (Mega et al, 2019). Interestingly, TaSnRK2.9, which is a sucrose non-fermenting 1-related protein kinase gene cloned from bread wheat, elevated ABA content in tobacco (Nicotiana tabacum) resulting in greater drought tolerance (Feng et al, 2019). Therefore, both gene editing and genetic engineering show promise in terms of achieving drought tolerance in wheat.…”

Section: Genome Engineering Techniquesmentioning

confidence: 99%

Recent Progress in Germplasm Evaluation and Gene Mapping to Enable Breeding of Drought-Tolerant Wheat

2020

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There is a need to increase wheat productivity to meet the food demands of the evergrowing human population. However, accelerated development of high yielding varieties is hindered by drought, which is worsening due to climate change. In this context, germplasm diversity is central to the development of drought-tolerant wheat. Extensive collections of these genetic resources are conserved in national and international genebanks. In addition to phenotypic assessments, the use of advanced molecular techniques (e.g., genotype by sequencing) to identify quantitative trait loci (QTLs) for drought tolerance related traits is useful for genome-and marker-assisted selection based approaches. Therefore, to assist wheat breeders at a critical time, we searched the recent peer-reviewed literature (2011-current), first, to identify wheat germplasm observed to be useful genetic sources for drought tolerance, and second, to report QTLs associated with drought tolerance. Though many breeders limit the parents used in breeding programs to a familiar core collection, the results of this review show that larger germplasm collections have been sources of useful genes for drought tolerance in wheat. The review also demonstrates that QTLs for drought tolerance in wheat are associated with diverse physio-morphological traits, at different growth stages. Here, we also briefly discuss the potential of genome engineering/editing to improve drought tolerance in wheat. The use of CRISPR-Cas9 and other gene-editing technologies can be used to fine-tune the expression of genes controlling drought adaptive traits, while high throughput phenotyping (HTP) techniques can potentially accelerate the selection process. These efforts are empowered by wheat researcher consortia.

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TaSnRK2.9, a Sucrose Non-fermenting 1-Related Protein Kinase Gene, Positively Regulates Plant Response to Drought and Salt Stress in Transgenic Tobacco

Cited by 53 publications

References 62 publications

Comprehensive Analysis of SnRK Gene Family and their Responses to Salt Stress in Eucalyptus grandis

Comprehensive Analysis of SnRK Gene Family and their Responses to Salt Stress in Eucalyptus grandis

Overexpression of Peroxidase Gene GsPRX9 Confers Salt Tolerance in Soybean

Recent Progress in Germplasm Evaluation and Gene Mapping to Enable Breeding of Drought-Tolerant Wheat

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