TAT conjugated, FITC doped silica nanoparticles for bioimaging applications

Santra, Swadeshmukul; Yang, Heesun; Dutta, Debamitra; Stanley, Jessie T.; Holloway, Paul H.; Tan, Weihong; Moudgil, Brij M.; Mericle, Robert A.

doi:10.1039/b411916a

Cited by 246 publications

(161 citation statements)

References 22 publications

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“…Peptides such as TAT have been used to enable transport of fluorescently labelled silica nanoparticles in vivo across the BBB [102]. In addition, polylysine has been used to coat iron oxide nanoparticles for their transport across the BBB [103,104].…”

Section: Peptide Coated Nanoparticlesmentioning

confidence: 99%

The role of peptides in blood‐brain barrier nanotechnology

Teixidó

Giralt

2007

Journal of Peptide Science

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Abstract:The blood-brain barrier (BBB) regulates the passage of molecules between the bloodstream and the brain. Overcoming the difficulty of delivery drugs to specific areas of the brain is a major challenge. The BBB exerts a neuroprotective function as it hinders the delivery of diagnostic and therapeutic agents to the brain. Here, we provide an overview of the way in which peptides and nanotechnology are being exploited in tandem to address this problem. Peptides can be used as specialised coatings able to transport nanoparticles with specific properties, such as targeting. The nanoparticle can also carry a peptide drug. Furthermore, peptides can be used in less conventional approaches such as all-peptide nanoparticles. In summary, the combined use of peptides and nanotechnology offers tremendous hope in the treatment of brain disorders.

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Section: Peptide Coated Nanoparticlesmentioning

confidence: 99%

The role of peptides in blood‐brain barrier nanotechnology

Teixidó

Giralt

2007

Journal of Peptide Science

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“…Very recently, our group reported the synthesis and characterization of monofunctionalized nonporous SiO 2 -NPs for MRI applications using Gd complexes . In one case, Santra et al (2004) have reported the coupling of additional functional groups, Tat peptide and fluorescein isothiocyanate (FITC), on SiO 2 -NPs (70 nm in diameter). They first prepared a silane precursor coupled to FITC to get fluorescently labeled NPs followed by conjugation of the Tat peptide via N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) coupling chemistry.…”

Section: Introductionmentioning

confidence: 99%

Multifunctional silica nanoparticles for optical and magnetic resonance imaging

Joshi

Feldmann

Koestner

et al. 2012

Biological Chemistry

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The surface of spherical, nonporous silica nanoparticles (SiO2-NPs) was modified with gadolinium (Gd) complexes, fluorophores, and cell-penetrating peptides to achieve multifunctionality on a single particle. The Gd surface concentrations were 9–16 μmol/g resulting in nanomaterials with high local longitudinal and transversal relaxivities (~1×105 and ~5×105 /mm/s/NP, respectively). Rapid cellular uptake was observed in vitro; however, larger extracellular agglomerates were also formed. In vivo administration revealed a fast distribution throughout the body followed by a nearly complete disappearance of fluorescence in all organs except the lungs, liver, and spleen after 24 h. Such NPs have the potential to serve as efficient multimodal probes in molecular imaging.

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“…ORMOSIL NPs conjugated with fluorophores and targeting ligands have been used in optical imaging of tumour cells in vitro [32]. The organic groups bonded to a siloxane skeleton besides retarding the solegel process of the organoalkoxysilane acts as fillers in the silica network, conferring some degree of flexibility to the otherwise rigid silica matrix, changing the porosity profile besides modifying the NPs colloidal chemistry.…”

Section: Introductionmentioning

confidence: 99%

Core–shell superparamagnetic nanoparticles with interesting properties as contrast agents for MRI

Carvalho

Domingues

Gonçalves

2015

Materials Chemistry and Physics

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h i g h l i g h t sCoreeshell nanoparticles with iron oxide cores and different coatings were obtained. The new nanosystems are efficient as negative contrast agent for MRI. The efficiency is discussed relating to the different coatings properties. Contrast enhancement is quantified in phantoms MRI and validated in an animal model. The potentiality of these new coreeshell NPs as negative CA for MRI is demonstrated and quantified. The longitudinal and transverse relaxivities of NPs with three different coating compositions were studied at a 7 T magnetic field: silica (TEOS), (3-aminopropyl) triethoxysilane (APTES) and (3-glycidoxypropyl) methyldiethoxysilane (GPTMS).Clearly, it was found that the coreeshell NPs efficiency as CA was strongly depend on the SPIONs coating.All the three coreeshell NPs studied presented a very small effect on the longitudinal relaxation time but a pronounced one on the transverse relaxation time, leading to a very high transverse longitudinal relaxivities ratio, decisive for their efficiency as negative CA for MRI.The effect of the coreeshell NPs on the MRI contrast enhancement is obtained and quantified in a set of MRI of agar phantoms obtained at 7 T magnetic field and with a imaging gradient field of 1.6 T/m. The coreeshell NPs were tested in Zebra-fish (Danio rerio) animal model. Zebra-fish MRI were obtained with animals injected with the three coreeshell NPs and the contrast enhancement validated.

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TAT conjugated, FITC doped silica nanoparticles for bioimaging applications

Cited by 246 publications

References 22 publications

The role of peptides in blood‐brain barrier nanotechnology

The role of peptides in blood‐brain barrier nanotechnology

Multifunctional silica nanoparticles for optical and magnetic resonance imaging

Core–shell superparamagnetic nanoparticles with interesting properties as contrast agents for MRI

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