TAT-modified serum albumin nanoparticles for sustained-release of tetramethylpyrazine and improved targeting to spinal cord injury

Wan, Yujie; Du, Xingjie; Li, Jian; Wei, Jun; Li, Ting; Li, Chunhong; Liu, Zhongbing; Zhou, Meiling; Zhong, Zhaohui

doi:10.1186/s12951-020-00766-4

Cited by 25 publications

(16 citation statements)

References 33 publications

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“…Compared with the positive control, RDMR showed less than 5% hemolysis rate with concentration up to 400 × 10 −9 m (Figure S7, Supporting Information), demonstrating the biocompatibility of the nanoparticles for intravenous injection. [ 63 ] To track the in vivo circulation, the miRNA was labeled with a red fluorescent Cy5.5. After injection, free miR‐124 was rapidly cleared from the mice body, showing a half‐life of 5.5 min ( Figure a).…”

Section: Resultsmentioning

confidence: 99%

Brain‐Penetration and Neuron‐Targeting DNA Nanoflowers Co‐Delivering miR‐124 and Rutin for Synergistic Therapy of Alzheimer's Disease

Ouyang

Zhu

et al. 2022

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tau hyperphosphorylation, mitochondrial dysfunction and oxidative stress, and neuroinflammatory response. [3] Among them, the amyloid theory that focuses on excessive deposition of β-amyloid (Aβ) has been extensively studied with wide acceptance. [4] In this theory, imbalance of Aβ metabolism and catabolism in the central nervous system (CNS) has been regarded as the main reason for AD-like pathology. [5] Aβ is the product of a proteolytic process of a transmembrane protein, amyloid precursor protein (APP), by β-secretase 1 (BACE1, β-site APP cleavage enzyme 1) and the γ-secretase. [6] The overgeneration and abnormal aggregation of Aβ 1-42 can cause the collapse of dendritic spines and neuronal loss, induce oxidative stress, and promote inflammatory by activating microglia, which in turn exacerbates the pathogenesis of AD. [7] Several strategies focus on blocking Aβ aggregation and alleviating Aβ-mediated neurotoxicity have been developed, including aggregation inhibition, copper chelation, Aβ photo-oxidation, fibril thermal dissociation, and Aβ degradation. [8] However, many Aβ targeting pharmacological treatments failed to slow down genitive decline or improve global functioning in phase 3 clinical trials although with definite efficacy at animal model. [9] Besides the pathological discrepancy between real clinical patient and AD animal model, [10] another important reason is that AD is a multifactorial disease and therapy targeting Aβ alone is far more enough. [11] Despite significant Alzheimer disease (AD) is the leading cause of dementia that affects millions of old people. Despite significant advances in the understanding of AD pathobiology, no disease modifying treatment is available. MicroRNA-124 (miR-124) is the most abundant miRNA in the normal brain with great potency to ameliorate AD-like pathology, while it is deficient in AD brain. Herein, the authors develop a DNA nanoflowers (DFs)-based delivery system to realize exogenous supplementation of miR-124 for AD therapy. The DFs with well-controlled size and morphology are prepared, and a miR-124 chimera is attached via hybridization. The DFs are further modified with RVG29 peptide to simultaneously realize brain-blood barrier (BBB) penetration and neuron targeting. Meanwhile, Rutin, a small molecular ancillary drug, is co-loaded into the DFs structure via its intercalation into the double stranded DNA region. Interestingly, Rutin could synergize miR-124 to suppress the expression of both BACE1 and APP, thus achieving a robust inhibition of amyloid β generation. The nanosystem could pro-long miR-124 circulation in vivo, promote its BBB penetration and neuron targeting, resulting in a significant increase of miR-124 in the hippocampus of APP/PS1 mice and robust therapeutic efficacy in vivo. Such a bio-derived therapeutic system shows promise as a biocompatible nanomedicine for AD therapy.

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Section: Resultsmentioning

confidence: 99%

Brain‐Penetration and Neuron‐Targeting DNA Nanoflowers Co‐Delivering miR‐124 and Rutin for Synergistic Therapy of Alzheimer's Disease

Ouyang

Zhu

et al. 2022

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“…The hemolytic potential of various concentrations and types of liposomes was measured using red blood cells [ 47 ]. Briefly, fresh blood was collected from female Sprague Dawley rat hearts and placed in anti-coagulant tubes.…”

Section: Methodsmentioning

confidence: 99%

Barrier-penetrating liposome targeted delivery of basic fibroblast growth factor for spinal cord injury repair

Wu¹,

Wang²,

Wei³

et al. 2023

Materials Today Bio

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Section: Methodsmentioning

confidence: 99%

“…The conditions for the detection of FLC were as follows: mobile phase, methanol/water (35 : 65, v/v); detection wavelength, 261 nm; flow rate, 1.0 mL min −1 ; column temperature, 40 °C; injection volume, 5 µL. EE and DL were calculated using the following formulas: 34 A dialysis experiment was conducted to evaluate the in vitro releases of Mag and FLC from PPi-Mag/FLC-LPs. Briefly, 1 mL solution of PPi-Mag/FLC-LPs, free Mag, or free FLC was put into a dialysis bag with a molecular weight cutoff of 3 kDa (Spectrum-Labs, Los Angeles, CA, USA).…”

Section: Characterization Of the Lpsmentioning

confidence: 99%

Biomineral-binding liposomes with dual antibacterial effects for preventing and treating dental caries

et al. 2023

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TAT-modified serum albumin nanoparticles for sustained-release of tetramethylpyrazine and improved targeting to spinal cord injury

Cited by 25 publications

References 33 publications

Brain‐Penetration and Neuron‐Targeting DNA Nanoflowers Co‐Delivering miR‐124 and Rutin for Synergistic Therapy of Alzheimer's Disease

Brain‐Penetration and Neuron‐Targeting DNA Nanoflowers Co‐Delivering miR‐124 and Rutin for Synergistic Therapy of Alzheimer's Disease

Barrier-penetrating liposome targeted delivery of basic fibroblast growth factor for spinal cord injury repair

Biomineral-binding liposomes with dual antibacterial effects for preventing and treating dental caries

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