Tat peptide-mediated cellular delivery: back to basics

Brooks, Hilary; Lebleu, Bernard; Vivès, Éric

doi:10.1016/j.addr.2004.12.001

Cited by 605 publications

(481 citation statements)

References 101 publications

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“…While the cell-penetrating property of Tat peptides have been extensively studied [41], [42] and [43], the potential of protamine to carry cargos across the cell membrane is less recognised. However, in 2005, two independent groups reported that protamine can act as an efficient membrane-translocating peptide both in vitro [44] and in vivo [44] and [45].…”

Section: Discussionmentioning

confidence: 99%

Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis

Gómez

Csaba

Fischer

et al. 2008

Journal of Controlled Release

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Surface modifications of poly(lactide-co-glycolide) microparticles with different polycationic electrolytes have mainly been studied for conjugation to antigens and/or adjuvants. However, the in vivo immunological effects of using surface charged particles have not been address yet. In this study, microparticles were coated or not with protamine, a cationic and arginine-rich electrolyte that confers microparticles with a positively surface charge. We then evaluated the potential of protamine-coatings to assist the induction of immune responses in mice. Interestingly, enhanced antibodies and T-cell responses were observed in mice treated with the coated particles. In vitro studies suggested that the improved immunological performance was mediated by an increased uptake. Indeed, protamine-coated particles that carried a plasmid were even internalised into non-phagocytic cells and to cause their transfection. These results open the way for further research into a novel technology that combines the use protamine for facilitated cell penetration of that and biodegradable microparticles for prolonged antigen or drug release.

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Section: Discussionmentioning

confidence: 99%

Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis

Gómez

Csaba

Fischer

et al. 2008

Journal of Controlled Release

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“…Since the cellular uptake of arginine-rich peptides is dependent on a variety of factors, including temperature, incubation time, cell type, cargo type and size, linkage type and size [4,61], comparison between different experiments have been difficult, and has compounded the controversy surrounding the uptake mechanism. It has no doubt been recognized that more than one mechanism may be involved in TAT translocation activity.…”

Section: Direct Translocation Endocytosis: An Either/or Discourse?mentioning

confidence: 99%

Arginine‐rich cell‐penetrating peptides

et al. 2009

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a b s t r a c tArginine-rich cell-penetrating peptides are short cationic peptides capable of traversing the plasma membranes of eukaryotic cells. While successful intracellular delivery of many biologically active macromolecules has been accomplished using these peptides, their mechanisms of cell entry are still under investigation. Recent dialogue has centered on a debate over the roles that direct translocation and endocytotic pathways play in internalization of cell-penetrating peptides. In this paper, we review the evidence for the broad range of proposed mechanisms, and show that each distinct process requires negative Gaussian membrane curvature as a necessary condition. Generation of negative Gaussian curvature by cell-penetrating peptides is directly related to their arginine content. We illustrate these concepts using HIV TAT as an example.

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“…In addition to targeting, these biologically active ligands can aid in cellular uptake [60]. As previously mentioned, Christian et al demonstrated that the highly cationic HIV-derived TAT peptide, when coupled to NIR-emissive polymersomes, enhances cellular delivery of polymer vesicles to dendritic cells while moderately affecting cell viability [19].…”

Section: Polymersome Surface Modifications For Delivery and Therapymentioning

confidence: 99%

Polymersomes: A new multi-functional tool for cancer diagnosis and therapy

Levine

Ghoroghchian

Freudenberg

et al. 2008

Methods

200

122

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Nanoparticles are being developed as delivery vehicles for therapeutic pharmaceuticals and contrast imaging agents. Polymersomes (mesoscopic polymer vesicles) possess a number of attractive biomaterial properties that make them ideal for these applications. Synthetic control over block copolymer chemistry enables tunable design of polymersome material properties. The polymersome architecture, with its large hydrophilic reservoir and its thick hydrophobic lamellar membrane, provides significant storage capacity for both water soluble and insoluble substances (such as drugs and imaging probes). Further, the brush-like architecture of the polymersome outer shell can potentially increase biocompatibility and blood circulation times. A further recent advance is the development of multi-functional polymersomes that carry pharmaceuticals and imaging agents simultaneously. The ability to conjugate biologically active ligands to the brush surface provides a further means for targeted therapy and imaging. Hence, polymersomes hold enormous potential as nanostructured biomaterials for future in vivo drug delivery and diagnostic imaging applications.

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Tat peptide-mediated cellular delivery: back to basics

Cited by 605 publications

References 101 publications

Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis

Surface coating of PLGA microparticles with protamine enhances their immunological performance through facilitated phagocytosis

Arginine‐rich cell‐penetrating peptides

Polymersomes: A new multi-functional tool for cancer diagnosis and therapy

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