Tau Differentially Regulates the Dynamic Localization of Early Endosomes and Lysosomes

Abstract

Endogenous electric fields are present in many biological processes including at the edge of healing wounds and can direct cell migration. Here we measure the effect of DC electric fields on actin polymerization dynamics. We find that actin waves are guided by DC electric fields for multiple cell types. Giant Dictyostelium discoideum cells (produced via electrofusion) allow us to measure electric field response of actin waves located far from the leading or trailing edge of a cell, and thus decouple cell polarity and shape from actin wave responses. We also use nanoridges, fabricated substrate nanotopographies, which alter the actin waves from the broad, two-dimensional waves seen on flat substrates to one-dimensional waves traveling along the nanoridges. Our data reveal new insights into the mechanism behind cell migration via DC electric fields with actin waves playing a key role in cell response and control.

Comparative interactome mapping of Tau‐protein in classical and rapidly progressive Alzheimer's disease identifies subtype‐specific pathways

“…Microtubule‐associated proteins including Tau are a key component of these regulatory networks. Aberrant Tau phosphorylation can disrupt the targeting of critical cargoes to their specific destinations [66]. Recently, it has been reported that patients with rpAD contain ~80% misfolded four‐repeat Tau (4R‐Tau) and ~20% of misfolded 3R‐Tau isoform with similar conformations [11].…”