Taura syndrome virus loads in Litopenaeus vannamei hemolymph following infection are related to differential mortality

Cao, Zhiming; Wang, Shiao Y.; Breeland, Verlee; Moore, Anne-Marie; Lotz, Jeffrey M.

doi:10.3354/dao02258

Cited by 8 publications

(3 citation statements)

References 20 publications

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“…Clinical symptoms of acute TSV infection in farmed P. vannamei are characterized by a reddish body color (especially on the tail; uropods, and appendages induced by chromatophore expansion) and irregular black (melanization) spots under the cuticle layer, in addition to anorexia, an erratic swimming behavior, lethargy, soft cuticles, anorexia, flaccid bodies and opaque musculature [ 95 , 129 ] ( Figure 19 ). Shrimp acutely infected with TSV persist for 1–10 days after infection, and exhibit TSV-specific histological lesions, and mortality occurs during or immediately after molting [ 134 , 135 ]. According to Dhar and Allnutt (2008) [ 130 ], TSV infection begins within 24 h and death peaks between 7–10 days, and naturally or experimentally surviving individuals with acute infections develop grossly visible, multifocal, melanized lesions on the cephalothorax, tail, and appendages [ 95 ].…”

Section: Rna Viral Diseasesmentioning

confidence: 99%

“…In the chronic stage of TSV infection, shrimp are asymptomatic, and the only histologically identifiable lesions are numerous lymphoid organ spheroids (LOS) [ 133 ]. Surviving individuals after TSV infection can act as life-long carriers of TSV infection, and the prevalence of TSV infection in farms can vary from 0–100% [ 134 ].…”

Section: Rna Viral Diseasesmentioning

confidence: 99%

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Viral Shrimp Diseases Listed by the OIE: A Review

Lee

Choi

et al. 2022

Viruses

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Shrimp is one of the most valuable aquaculture species globally, and the most internationally traded seafood product. Consequently, shrimp aquaculture practices have received increasing attention due to their high value and levels of demand, and this has contributed to economic growth in many developing countries. The global production of shrimp reached approximately 6.5 million t in 2019 and the shrimp aquaculture industry has consequently become a large-scale operation. However, the expansion of shrimp aquaculture has also been accompanied by various disease outbreaks, leading to large losses in shrimp production. Among the diseases, there are various viral diseases which can cause serious damage when compared to bacterial and fungi-based illness. In addition, new viral diseases occur rapidly, and existing diseases can evolve into new types. To address this, the review presented here will provide information on the DNA and RNA of shrimp viral diseases that have been designated by the World Organization for Animal Health and identify the latest shrimp disease trends.

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Section: Rna Viral Diseasesmentioning

confidence: 99%

Section: Rna Viral Diseasesmentioning

confidence: 99%

Viral Shrimp Diseases Listed by the OIE: A Review

Lee

Choi

et al. 2022

Viruses

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“…To overcome this PCR inhibition problem, the efficacy of a PCR additive reagent was tested. The reagent, containing trehalose, bovine serum albumin (BSA), and polysorbate‐20 (Tween‐20) (TBT‐PAR), has been routinely used for the detection of a virus in shrimp samples (Cao et al, 2010). Trehalose stabilizes proteins and backbone fluctuations of DNA (cf.…”

mentioning

confidence: 99%

Enhancing PCR amplification of DNA from recalcitrant plant specimens using a trehalose‐based additive

2013

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• Premise of the study: PCR amplification of DNA extracted from plants is sometimes difficult due to the presence of inhibitory compounds. An effective method to overcome the inhibitory effect of compounds that contaminate DNA from difficult plant specimens is needed.• Methods and Results: The effectiveness of a PCR additive reagent containing trehalose, bovine serum albumin (BSA), and polysorbate-20 (Tween-20) (TBT-PAR) was tested. PCR of DNA extracted from fresh, silica-dried, and herbarium leaf material of species of Achariaceae, Asteraceae, Lacistemataceae, and Samydaceae that failed using standard techniques were successful with the addition of TBT-PAR.• Conclusions: The addition of TBT-PAR during routine PCR is an effective method to improve amplification of DNA extracted from herbarium specimens or plants that are known to contain PCR inhibitors.

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