Taurine and Beta-Alanine Uptake in Cultured Human Skin Fibroblasts from Patients with Friedreich's Ataxia

Mélancon, Serge B.; Grignon, B.; Potier, Michel; Dallaire, Louis

doi:10.1017/s0317167100119730

Cited by 4 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results support our previous data on the kinetics of /?-amino acid uptake (Melancon et al, 1979a) and confirm that Tau and Bala are normally transported in cultured skin fibroblasts of patients with F.A. The apparent discrepancy between urinary findings (Lemieux et al, 1976;Filla et al, 1979) and our fibroblast studies can be explained in many ways.…”

Section: Discussionsupporting

confidence: 88%

“…Beta-amino-isobutyric acid would seem to be incorporated into cultured skin fibroblasts through a third and as yet unknown transport system unrelated to system "Beta" and "L". Our previously reported data on the kinetics of Tau and Bala uptake in cultured skin fibroblasts (Melancon et al, 1979a) would then be valid only for Tau as this /3-amino acid is incorporated through a single transport system in fibroblasts. The observed Km and Vmax for Bala uptake included Bala transport via systems "Beta" and "L" together in a proportion of four to one and these figures should probably be examined again.…”

Section: Discussionmentioning

confidence: 99%

“…In a previous study, we compared the uptake kinetics of Tau and Bala using cultured skin fibroblasts from patients with F.A. and control individuals (Melancon et al, 1979a).…”

mentioning

confidence: 99%

See 2 more Smart Citations

The Beta-Amino Acid Transport System in Friedreich's Ataxia

Mélancon

Grignon

Ledru

et al. 1980

Can. j. neurol. sci.

Self Cite

View full text Add to dashboard Cite

SUMMARY:Taurine and β-amino uptake in cultured skin fibroblasts proceeds through at least two distinct amino acid transport systems. The predominant Bamino acid uptake system which we refer to as the “Beta” system, incorporates taurine in a proportion of 95%, β-alanine in a proportion of 80% and does not incorporate β-amino-isobutyric acid. A second transport system for β-alanine seems to be operative in cultured skin fibroblasts and this system shares the characteristics of system “L” for branched-chain and ringside neutral amino acids. Results of ion depletion experiments, metabolic inhibition by drugs and blocking agents and previous kinetic studies of taurine and β-alanine uptake in cultured skin fibroblasts failed to disclose any major difference in β-amino acid transport between control individuals and patients with Friedreich's ataxia.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

The Beta-Amino Acid Transport System in Friedreich's Ataxia

Mélancon

Grignon

Ledru

et al. 1980

Can. j. neurol. sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…These osmolytes are part of stress response of NHKs to UVA radiation. Taurine and myo-inositol are also taken up by human skin fibroblasts and fibroblast lines (14)(15)(16). Taurine and myo-inositol are also taken up by human skin fibroblasts and fibroblast lines (14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

“…Accordingly, UVA induces the expression of the osmolyte transporter BGT-1, SMIT and TAUT in NHKs which leads to an increased uptake of osmolytes, especially taurine (13). Taurine and myo-inositol are also taken up by human skin fibroblasts and fibroblast lines (14)(15)(16). Interestingly, taurine release is induced by oxidative stress and inhibited by antioxidants in NIH3T3 mouse fibroblasts (17).…”

Section: Introductionmentioning

confidence: 99%

Ultraviolet A induces transport of compatible organic osmolytes in human dermal fibroblasts

Warskulat¹,

Brookmann²,

Felsner

et al. 2008

Experimental Dermatology

View full text Add to dashboard Cite

Compatible organic osmolytes, such as betaine, myo-inositol and taurine, are involved in cell protection. Human dermal fibroblasts accumulate these osmolytes and express mRNA specific for their transporting systems betaine-/gamma-amino-n-butyric acid (GABA) transporter (BGT-1), sodium-dependent myo-inositol transporter (SMIT) and taurine transporter (TAUT). Taurine uptake was about sixfold higher than that of betaine and myo-inositol. Compared with normoosmotic (305 mOsm/l) control, hyperosmotic exposure (405 mOsm/l) led to a twofold induction of osmolyte uptake. Ultraviolet A (UVA) upregulated osmolyte transporter mRNA levels and increased osmolyte uptake. Taurine inhibited UVA-induced interleukin-6 (Il-6) mRNA expression by 40%. Furthermore, Il-6 accumulation in the supernatants of UVA-irradiated dermal fibroblasts was much slower when cells were preincubated with taurine. These data indicate that taurine accumulation seems to be part of the fibroblast response to UVA radiation and may protect against UVA-induced Il-6 overexpression.

show abstract

Platelet taurine content in Friedreich's disease

Rodrigue

Bélanger

Gelder

et al. 1984

Can. J. Neurol. Sci.

View full text Add to dashboard Cite

We have studied the concentrations of taurine and of 6 other amino acids in platelets from 12 patients with Friedreich's disease and 12 age sex-matched normal control subjects. No significant differences could be demonstrated between the two groups. The glycine/serine ratio was lower in all the patients but this change did not reach statistical significance. From these and other data, we conclude that the taurine retention deficit observed previously in Friedreich's disease cannot be the primary causal defect. RESUME: Nous avons etudie les concentrations de la taurine et de six autres acides amines dans les plaquettes de 12 patients avec la maladie de Friedreich et de 12 tSmoins normaux du meme age et sexe. Nous n'avons pas mis en Evidence de difference significative entre les deux groupes. Le rapport glycine/serine etait uniformement plus bas chez les patients, mais cette modification n'atteignait pas une signification statistique. Nous concluons que le deficit dans la retention de la taurine, que nous avions prealablement mis en Evidence dans la maladie de Friedreich, ne peut etre causal a la maladie.

show abstract

Taurine and Beta-Alanine Uptake in Cultured Human Skin Fibroblasts from Patients with Friedreich's Ataxia

Cited by 4 publications

References 10 publications

The Beta-Amino Acid Transport System in Friedreich's Ataxia

The Beta-Amino Acid Transport System in Friedreich's Ataxia

Ultraviolet A induces transport of compatible organic osmolytes in human dermal fibroblasts

Platelet taurine content in Friedreich's disease

Contact Info

Product

Resources

About