Taurine chloramine induces apoptosis in human osteosarcoma cell lines

Abstract

Although combination of surgery with chemotherapy has noticeably improved the survival rate of osteosarcoma patients, the application of anticancer drugs is still associated with significant adverse reactions, for instance acquisition of drug-resistant phenotypes, necessitating the development of new chemotherapeutical agents. Therefore, the aim of this study was to research, if taurine chloramine (NCT) induces apoptosis in the osteosarcoma cell lines HOS, MG-63, and SAOS-2. Proliferation of osteosarcoma cells was detected with the ''EZ4U Cell Proliferation and Cyotoxicity Assay'' showing a time-and dose-dependent cytotoxic effect of NCT on these cell lines. After 3 h of incubation all cell lines showed significantly less cells at 5.5 mM NCT solutions, after 6 h at concentrations of 1.1 and 2.2 mM. Acridine-orange fluorescence nuclear staining showed characteristic features of apoptosis. DNA fragmentation was detected via ELISA, showing significant results for HOS and MG-63 after 6 h at an NCT concentration of 3.3 mM. Results of JC-1 mitochondrial FACS analysis presented a significant increase in apoptotic cells after 6 h at 3.3 mM for the tested cell lines. Summarized, the results of this study indicate that NCT is a promising agent in osteosarcoma therapy. ß

New Treatment Options for Osteosarcoma – Inactivation of Osteosarcoma Cells by Cold Atmospheric Plasma

“…OS therapy is complicated by the development of adverse effects that result from treatment with aggressive cytotoxic chemotherapeutic agents (9). Furthermore, therapy resistance of human osteosarcoma with poor prognosis has been reported in certain patient subgroups, necessitating new therapeutic options (14,15). Hence the present study evaluated for the first time the impact of CAP on the treatment of OS and found it a promising alternative therapeutic approach or favorable additional therapeutic option.…”

A Key Metabolic Regulator of Bone and Cartilage Health

“…For example, taurolidine is cytotoxic to osteosarcoma cells and supports the effect of doxorubicin and carboplatin [107]. In addition, taurine chloramine exerts cytotoxic effects against osteosarcoma cell lines (HOS, SAOS-2, and MG-63) by inducing the loss of mitochondrial membrane potential, activating the intrinsic mitochondrial apoptosis pathway [108].…”

Role of taurine, its haloamines and its lncRNA TUG1 in both inflammation and cancer progression. On the road to therapeutics? (Review)

“…Even though TauCl has been reported to hinder the proliferation of TNF-α stimulated neutrophils by inducing mitochondrial apoptosis ( 262 ), TauCl has been shown to confer protection to phagocytic cells from cell death caused by the overproduction of O 2 − and H 2 O 2 in an inflammatory milieu. Specifically, TauCl has been shown to reduce the proliferation in mitogen-stimulated human peripheral blood lymphocytes ( 223 ), in IL-3-dependent murine hema-topoietic prolymphocytic B cells ( 263 ), in human skin fibroblasts ( 264 ) and tumor cells (B lymphoma osteosarcoma cell lines) ( 265 , 266 ). Growth-inhibitory effect of TauCl was presented in distinct cell types in response to various stimuli, confirming that there are conserved mechanisms that warrant further investigation.…”