Taurine grafting and collagen adsorption on PLLA films improve human primary chondrocyte adhesion and growth

Pellegrino, Luca; Cocchiola, Rossana; Francolini, Iolanda; Lopreiato, Mariangela; Piozzi, Antonella; Zanoni, R.; d’Abusco, Anna Scotto; Martinelli, Andrea

doi:10.1016/j.colsurfb.2017.07.047

Cited by 15 publications

(19 citation statements)

References 34 publications

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“…PLA is a hydrophobic polymer, which is a limiting factor for cell attachment. To address this, surface treatments are required to increase the substrate wettability [10], [11]. The most widely utilised and preferred surface modification methods rely on oxygen plasma and UV-ozone treatments [12].…”

Section: Pla Functionalisationmentioning

confidence: 99%

“…In conclusion, the proposed functionalisation protocol allows one to tune the surface wettability of PLA to obtain the desired contact angle for cell culture. The resulting formation of the -COOH and of -OH groups at the surface can be readily used for further conjugation of surface modifying species in order to provide the best environment for the specific cell culture application [11], [14].…”

Section: Pla Functionalisationmentioning

confidence: 99%

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Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications

et al. 2020

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Organ-on-chips are miniaturised devices aiming at replacing animal models for drug discovery, toxicology and studies of complex biological phenomena. The field of Organ-On-Chip has grown exponentially, and has led to the formation of companies providing commercial Organ-On-Chip devices. Yet, it may be surprising to learn that the majority of these commercial devices are made from Polydimethylsiloxane (PDMS), a silicone elastomer that is widely used in microfluidic prototyping, but which has been proven difficult to use in industrial settings and poses a number of challenges to experimentalists, including leaching of uncured oligomers and uncontrolled adsorption of small compounds. To alleviate these problems, we propose a new substrate for organ-on-chip devices: Polylactic Acid (PLA). PLA is a material derived from renewable resources, and compatible with high volume production technologies, such as microinjection moulding. PLA can be formed into sheets and prototyped into desired devices in the research lab. In this article we uncover the suitability of Polylactic acid as a substrate material for Microfluidic cell culture and Organ-on-a-chip applications. Surface properties, biocompatibility, small molecule adsorption and optical properties of PLA are investigated and compared with PDMS and other reference polymers.

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Section: Pla Functionalisationmentioning

confidence: 99%

Section: Pla Functionalisationmentioning

confidence: 99%

Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications

et al. 2020

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“…In many papers, surface modification with aminolysis reaction is reported for polycaprolactone [14,29,30,31,32,33,34,35]. A smaller number of studies is related to the surface functionalization of other polyesters, such as polylactide [36,37,38] and poly(lactide- co -caprolactone) [19,39,40,41]. Moreover, in the case of PLLA meshes, aminolysis is frequently used as a method of fiber fragmentation [42,43,44,45,46].…”

Section: Introductionmentioning

confidence: 99%

Aminolysis of Various Aliphatic Polyesters in a Form of Nanofibers and Films

Jeznach

Kołbuk

2019

Polymers

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Surface functionalization of polymer scaffolds is a method used to improve interactions of materials with cells. A frequently used method for polyesters is aminolysis reaction, which introduces free amine groups on the surface. In this study, nanofibrous scaffolds and films of three different polyesters–polycaprolactone (PCL), poly(lactide-co-caprolactone) (PLCL), and poly(l-lactide) (PLLA) were subjected to this type of surface modification under the same conditions. Efficiency of aminolysis was evaluated on the basis of ninhydrin tests and ATR–FTIR spectroscopy. Also, impact of this treatment on the mechanical properties, crystallinity, and wettability of polyesters was compared and discussed from the perspective of aminolysis efficiency. It was shown that aminolysis is less efficient in the case of nanofibers, particularly for PCL nanofibers. Our hypothesis based on the fundamentals of classical high speed spinning process is that the lower efficiency of aminolysis in the case of nanofibers is associated with the radial distribution of crystallinity of electrospun fiber with more crystalline skin, strongly inhibiting the reaction. Moreover, the water contact angle results demonstrate that the effect of free amino groups on wettability is very different depending on the type and the form of polymer. The results of this study can help to understand fundamentals of aminolysis-based surface modification.

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“…PLLA is a biocompatible and biodegradable enantiomeric polyester produced from lactic acid synthesis [26]. Further, PLLA presents a broad spectra of processing morphologies, including films [27,28], electrospun fibers [29,30] and membranes [31], as well as electroactive characteristics, with a piezoelectric constant of about 6-10 pC.N -1 [32], enabling the possibility to integrate smart and responsive materials in battery applications [33]. The suitable characteristics of PLLA have been demonstrated in its applications in the field of tissue and biomedical engineering [34], agriculture [35], aquaculture [36], sensors [37] and electronics [37].…”

Section: Introductionmentioning

confidence: 99%

Lithium-ion battery separator membranes based on poly(L-lactic acid) biopolymer

Barbosa

Reizabal

Correia

et al. 2020

Materials Today Energy

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Sustainable materials are increasingly needed in lithium ion batteries in order to reduce their environmental impact and improve their recyclability. This work reports on the production of separators using poly (L-lactic acid) (PLLA) for lithium ion battery applications. PLLA separators were obtained by solvent casting technique, by varying polymer concentration in solution between 8 wt.% and 12 wt.% in order to evaluate their morphology, thermal, electrical and electrochemical properties. It is verified that morphology and porosity can be tuned by varying polymer concentration and that the separators are thermally stable up to 250 ºC. The best ionic conductivity of 1.6 mS/cm was obtained for the PLLA separator prepared from 10 wt.% polymer concentration in solution, due to the synergistic effect of the morphology and electrolyte uptake. For this membrane, a high discharge capacity value of 93 mAh.g -1 was obtained at the rate of 1C.In this work, it is demonstrated that PLLA is a good candidate for the development of separator membranes, in order to produce greener and environmentally friendly batteries in a circular economy context.

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Taurine grafting and collagen adsorption on PLLA films improve human primary chondrocyte adhesion and growth

Cited by 15 publications

References 34 publications

Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications

Polylactic is a Sustainable, Low Absorption, Low Autofluorescence Alternative to Other Plastics for Microfluidic and Organ-on-Chip Applications

Aminolysis of Various Aliphatic Polyesters in a Form of Nanofibers and Films

Lithium-ion battery separator membranes based on poly(L-lactic acid) biopolymer

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