This study aimed to assess infestation levels of the mite Varroa destructor and microspore Nosema spp. in apiaries in the Picos microregion of Piauí state, Brazil, during the dry season. To that end, bees were collected (n=31) from 19 apiaries between September and October 2016. Infestation levels of the parasites were analyzed at the Laboratory of the Bee Technology Center, an institution affiliated with the Senador Helvídio Nunes de Barros Campus of the Federal University of Piauí. Varroa destructor was identified in 54% of the colonies, with infestation levels varying from 0 to 10.54%, and an average of 3.31% ± 4.12. Analysis of Nosema spp. revealed that all the colonies sampled exhibited microsporidia, albeit at low (1 to 1.4 spores/bee) and very low levels (0.35 to 0.95 spores/bee) of infestation. Given the relevance of beekeeping in the semiarid region of Piauí, it was concluded that although infestation levels were deemed low, there is a need to regularly monitor the health of colonies in the apiaries.