Taxifolin Potently Diminishes Levels of Reactive Oxygen Species in Living Cells Possibly by Scavenging Peroxyl Radicals

Abstract

Taxifolin, a naturally occurring dihydroflavonol, has been reported to possess antioxidant activity, whereas there is little information regarding its antioxidant potency as a flavonoid. In the present study, the antioxidant efficacy of taxifolin in living cells was compared to that of catechin, a potent radical scavenger, by using flow cytometry with the redox-sensitive fluorescent dye, 2′,7′-dichlorodihydrofluorescein diacetate. Further, diminishing efficacy of taxifolin on peroxyl radicals was assessed by using an electron spin resonance spin-trapping technique. Taxifolin significantly reduced tert-butyl hydroperoxide-induced increases in Reactive Oxygen Species (ROS) levels in Caco-2 cells; the effect of taxifolin was stronger than that of catechin. Taxifolin also scavenged peroxyl (ROO•) radicals with statistically significance compared to that of catechin. This study showed that taxifolin has the potential to diminish ROS levels in living cells, possibly by scavenging ROO•.

Nailfold Capillaroscopy: A Comprehensive Review on Its Usefulness Not Only in Clinical Diagnosis, but Also in Unhealthy Dietary Lifestyle Improvements

“…We briefly summarize the examples of the biological effects of (−)-epicatechin as well as (+)taxifolin and/or (−)-taxifolin that were found in the experimental models and that may be relevant for the treatment of hypertension and viral infection through improvements of peripheral vascular function and anti-inflammatory activity as shown in Bernatova and Liskova's paper [105], and references of peripheral vascular function, cardioprotective-and anti-inflammatory effects were summarized in Table 8 [105,. References of cellular and molecular mechanisms underlying the biological effects of epicatechin and taxifolin are listed in Table 9 [105,113,117,121,126,128,[138][139][140][141][142][143][144][145][146][147][148][149][150], and these two flavonoids are effective in ROS (reactive oxygen species) reduction, antioxidant enzymes activation, endothelial NO elevation, and NF-kB reduction.…”

Nailfold Capillaroscopy: A Comprehensive Review on Its Usefulness in Both Clinical Diagnosis and Improving Unhealthy Dietary Lifestyles

“…Reported effects on peripheral vascular function and cardioprotective- and anti-inflammatory effects are summarized in Table 8 [ 107 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 , 137 , 138 , 139 ]. References to the cellular and molecular mechanisms underlying the biological effects of epicatechin and taxifolin are listed in Table 9 [ 107 , 115 , 119 , 123 , 128 , 130 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 ], including effects on reactive oxygen species (ROS) reduction, activation of anti-oxidant enzymes, endothelial NO elevation, and NF-κB reduction.…”

“…Reported effects on peripheral vascular function and cardioprotective-and anti-inflammatory effects are summarized in Table 8 [107,. References to the cellular and molecular mechanisms underlying the biological effects of epicatechin and taxifolin are listed in Table 9 [107,115,119,123,128,130,[140][141][142][143][144][145][146][147][148][149][150][151][152], including effects on reactive oxygen species (ROS) reduction, activation of anti-oxidant enzymes, endothelial NO elevation, and NF-κB reduction. Table 9.…”

Behavioral and neuropathological features of Alzheimer’s disease are attenuated in 5xFAD mice treated with intranasal GHK peptide

“…A previous search, using the Connectivity Map developed by the Broad Institute, found that GHK could alleviate impairment of TGFβ1 signaling (Pickart et al, 2017), a pathway associated with Aβ deposition and neurofibrillary tangle formation (Bosco et al, 2013). In addition, GHK has been shown to be an endogenous antioxidant by decreasing hydroxyl and peroxyl radicals (Sakuma et al, 2018), and improve cognitive performance in aging mice (Rosenfeld et al, 2023).…”