Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway

Tang, Zhenqiu; Yang, Chunjuan; Zuo, Baoyan; Zhang, Yanan; Wu, Gaosong; Wang, Yudi; Wang, Zhibin

doi:10.7717/peerj.6383

Cited by 26 publications

(18 citation statements)

References 38 publications

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“…There are no studies in the literature on the effect of taxifolin on LPO in cochlear tissue. However, recent studies have shown that taxifolin inhibits MDA increase in blood serum and heart tissue (20,21). This information obtained from the literature shows that previous studies support the results of our biochemical and histopathological experiments.…”

Section: Histopathological Resultssupporting

confidence: 91%

The Effect of Taxifolin on Cisplatin Induced Oxidative Cochlear Damage in Rats: Biochemical and Histopathological Examination

Erhan¹,

Bayram²,

Çimen³

et al. 2019

Acta Poloniae Pharmaceutica - Drug Research

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Ototoxicity of cisplatin is one of the major dose-limiting side effects. Cisplatin-dependent ototoxicity is known to be associated with reactive oxygen species. Protective effect of taxifolin, being a flavanone found in onions, milk thistle, French maritime, and Douglas fir bark, against cisplatin-associated ototoxicity will be examined in this study. There are no studies in the literature examining the protective effect of taxifolin against cisplatin-induced ototoxicity. 50 mg/kg taxifolin was orally administered to TXC group rats (n-6), and distilled water was orally administered as a solvent to CG (n-6) and HG (n-6) groups. One hour later, 5 mg/kg cisplatin was administered intraperitoneally (i.p) to TXC and CG groups. This procedure was repeated once a day for 7 days. At the end of this period, all animals were sacrificed by high-dose anesthesia (50 mg/kg thiopental sodium) and biochemical and histopathological examinations were performed on the dissected cochlear tissue. Our biochemical test results showed that oxidant parameters were significantly increased whereas antioxidant parameters were significantly decreased in the cisplatin group (CG) compared to taxifolin (TXC) and healthy (HG) groups (p < 0.0001 for both parameters). Histopathological results showed that severe cochlear vestibular membrane degeneration, dilated conjunctival blood vessels, edema and destruction developed in the cisplatin group. However, no pathological findings were found in the taxifolin-treated group except for mild degeneration and edema. This information suggests that taxifolin may be useful in the treatment of cisplatinassociated oxidative cochlear damage.

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Section: Histopathological Resultssupporting

confidence: 91%

The Effect of Taxifolin on Cisplatin Induced Oxidative Cochlear Damage in Rats: Biochemical and Histopathological Examination

Erhan¹,

Bayram²,

Çimen³

et al. 2019

Acta Poloniae Pharmaceutica - Drug Research

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“…El doble producto (DP) se encuentra disminuido en los procesos de isquemia reperfusión [9] y esto quedó demostrado en la DO del corazón perfundido a la velocidad de 10.5mL/min, en donde, al minuto 70, disminuyó hasta un valor de 10361 ± 2260, en comparación con el control que mantiene un valor de 22391 ± 2286 (Fig 1C). En la figura 1D se representan los resultados del doble productos para corazones sometidos a una perfusión a 12 mL/ min.…”

Section: Resultados a Determinación De Los Parámetros Cardiacosunclassified

Estandarización del modelo langendorff de corazón aislado para evaluar los daños inducidos por isquemia-reperfusión

Mero

Guerrero

Moráles

et al. 2021

apanac

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El modelo de isquemia-reperfusión representa una vía experimental para reproducir las características de un evento isquémico cardiaco agudo. Nuestro objetivo era validar el modelo Langendorff en corazón aislado de rata. Extrajimos el corazón y lo conectamos a un sistema de perfusión para evaluar la viabilidad del tejido, en donde modificamos la velocidad de perfusión (10.5 y 12.0 mL/min) y por otra parte, la inducción de la isquemia, que se realizó mediante la suspensión de la perfusión o la deprivación del oxigeno en la solución perfundida. Los parámetros cardiacos controles se obtuvieron justo antes de generar la isquemia y los 70 y 120 minutos de iniciada la perfusión. En todos los casos, mantuvimos un grupo control donde la perfusión fue continua, igual que la oxigenación del tejido cardiaco. En la determinacion del % de área de infarto se encontró que este valor fue de aproximadamente 5% para ambos controles. En la isquemia inducida por la suspension de la perfusion la velocidad de 10.5 fue de 22% y un 38% en la velocidad de 12 mL/min. Cuando la isquemia fue inducida por deprivación de oxigeno los grupos de 10.5 y 12 ml/min indujeron un 44% y 58%, respectivamente.Logramos concluir que la viabilidad del tejido es más estable cuando se perfunde a una velocidad de 10.5 mL/min, y que la isquemia se alcanza con mayor fiabilidad cuando se interrupe de la oxigenación del tejido.

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“…It inhibits the activity of oxidases reducing excessive production of reactive oxygen species (ROS). As it can reduce oxidative stress damage, it can improve rat ischemia-reperfusion injury by inhibiting the mitochondrial apoptosis pathway [ 39 ]. Sun et al found that TAX can improve diabetic cardiomyopathy by regulating oxidative stress and apoptosis in cardiomyocytes [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Exploring the Molecular Mechanism of Action of Yinchen Wuling Powder for the Treatment of Hyperlipidemia, Using Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation

2021

BioMed Research International

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Background. Yinchen Wuling powder is often used to treat clinical hyperlipidemia, although its mechanism of action remains unclear. In this study, we aimed to investigate the active ingredients found in Yinchen Wuling powder and find its mechanism of action when treating hyperlipidemia, using a combination of network pharmacology, molecular docking, and molecular dynamics simulation approaches. Methods. The TCMSP database was used to obtain the principle active ingredients found in Yinchen Wuling powder and the NCBI and DisGeNet databases were used to obtain the main target genes involved in hyperlipidemia, and the intersectional targets were obtained by EXCEL. We also used Cytoscape 3.7.2 software to construct a “Traditional Chinese Medicine-Active Ingredient-Target” network and use STRING platform to conduct “protein-protein interactional” (PPI) analyses on the intersection targets. Bioconductor software and RX 64 4.0.0 software were then used to perform GO functional enrichment analysis and KEGG pathway enrichment analysis on the targets. Molecular docking of core protein-ligand interactions was modeled using AutoDock Vina software. A simulation of molecular dynamics was conducted for the optimal core protein-ligand obtained by molecular docking using Amber18 software. Results. A total of 63 active ingredients were found in Yinchen Wuling powder, corresponding to 175 targets, 508 hyperlipidemia targets, and 55 intersection targets in total. Cytoscape 3.7.2 showed that the key active ingredients were quercetin, isorhamnetin, taxifolin, demethoxycapillarisin, and artepillin A. The PPI network showed that the key proteins involved were AKT1, IL6, VEGFA, and PTGS2. GO enrichment analysis found that genes were enriched primarily in response to oxygen levels and nutrient levels of the vesicular lumen and were associated with membrane rafts. These were mainly enriched in AGE-RAGE (advanced glycation end products-receptor for advanced glycation end products) signaling pathway in diabetic complications, fluid shear stress, and atherosclerosis, as well as other pathways. The molecular docking results indicated key binding activity between PTGS2-quercetin, PTGS2-isorhamnetin, and PTGS2-taxifolin. Results from molecular dynamics simulations showed that PTGS2-quercetin, PTGS2-isorhamnetin, and PTGS2-taxifolin bound more stably, and their binding free energies were PTGS2-quercetin -29.5 kcal/mol, PTGS2-isorhamnetin -32 kcal/mol, and PTGS2-taxifolin -32.9 kcal/mol. Conclusion. This study is based on network pharmacology and reveals the potential molecular mechanisms involved in the treatment of hyperlipidemia by Yinchen Wuling powder.

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Taxifolin protects rat against myocardial ischemia/reperfusion injury by modulating the mitochondrial apoptosis pathway

Cited by 26 publications

References 38 publications

The Effect of Taxifolin on Cisplatin Induced Oxidative Cochlear Damage in Rats: Biochemical and Histopathological Examination

The Effect of Taxifolin on Cisplatin Induced Oxidative Cochlear Damage in Rats: Biochemical and Histopathological Examination

Estandarización del modelo langendorff de corazón aislado para evaluar los daños inducidos por isquemia-reperfusión

Exploring the Molecular Mechanism of Action of Yinchen Wuling Powder for the Treatment of Hyperlipidemia, Using Network Pharmacology, Molecular Docking, and Molecular Dynamics Simulation

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