Taxol content in the bark of Himalayan Yew in relation to tree age and sex

Nadeem, Mohammad

doi:10.1016/s0031-9422(02)00115-2

Cited by 83 publications

(47 citation statements)

References 20 publications

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“…The fungus was inoculated in S-7 basal liquid medium (pH 6.8) [9] and incubated at 28ºC for 3 weeks under stationary condition for determining the taxol production. The putative taxol extracted from the fermentation broth and mycelium was analysed and quantified by HPLC [20] and LC-MS. Briefly, HPLC was carried out by injecting 20 µL of purified sample to HPLC (Waters Acquity HPLC, USA) equipped with a reverse phase column (RP-18 column, Waters, USA) and detected with online DAD (diode array detector) detector set at wavelength of 232 nm.…”

Section: Start Strainmentioning

confidence: 99%

Enhancement of taxol production from endophytic fungus Fusarium redolens

Garyali

Kumar

Reddy

2014

Biotechnol Bioproc E

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The optimization of taxol production by Fusarium redolens by one factor at a time (OFAT) approach led to production of 70 µg/L of taxol. With sucrose and NH 4 NO 3 as the carbon and nitrogen sources and medium volume (V m ) to flask volume (V f ) ratio of 0.2, a greater taxol production was attained. NH 4 NO 3 , MgSO 4 ·7H 2 O and NaOAc at 6.25, 0.63, and 1.25 g/L, were the significant factors for attaining the highest taxol production. The optimization of culture variables led to the production of taxol from 66 to 198 µg/L, which is three fold higher than that in the unoptimized medium. Current study results suggested the success of Response Surface Methodology in enhancing the production of fungal taxol.

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Section: Start Strainmentioning

confidence: 99%

Enhancement of taxol production from endophytic fungus Fusarium redolens

Garyali

Kumar

Reddy

2014

Biotechnol Bioproc E

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“…Analysis of extracts from Taxus species is usually made on C 18 (Ballero et al, 2003;Nadeem et al, 2002), C 8 (Lauren et al, 1995), phenyl (Theodoridis et al, 1998;Bala et al, 1999), biphenyl (Kethchum and Gibson, 1993) and cyano phases (Auriola et al, 1992). In most studies, C 18 columns were used to analyze samples.…”

Section: Simultaneous Determination Of Main Taxoids Inmentioning

confidence: 99%

“…In previous studies, the analysis of taxoids from plant materials was performed using C 18 columns and acetonitrile or methanol with an efficacy of approximately 50% in the mobile phase (Ballero et al, 2003;Mroczek and Glowniak, 2001;Nadeem et al, 2002;Van Rozendaal et al, 2000). However, it was difficult to separate paclitaxel, 7-xyl-10-DAT, 10-DAT and 10-DAB III with C 18 columns in the mobile phase.…”

mentioning

confidence: 99%

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Sun

et al. 2008

Biomedical Chromatography

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A simple and accurate RP-HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10-deacetylbaccatin III (10-DAB III), 7-xylosyl-10-deacetyltaxol (7-xyl-10-DAT), 10-deacetyltaxol (10-DAT), cephalomannine and 7-epi-10-deacetyltaxol (7-epi-10-DAT), in the extracts from the needles of three Taxus species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD < 2.61%), repeatability (RSD < 2.92%) and recovery (95.19-104.47%). The above taxoids in the extracts of Taxus cuspidata, T. chinensis and T. media were analyzed with the developed RP-HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10-DAB III, 7-xyl-10-DAT and 7-epi-10-DAT appeared in T. chinensis, while T. media possessed the highest content of 10-DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of Taxus species.

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“…There are also differences in the production of adventitious roots from cuttings collected from male and female T. baccata individuals depending on the type of auxin used; however, the mechanism of this reaction is not entirely clear (Nandi et al, 1996). Studies on carbon and nitrogen content have demonstrated the presence of comparable concentrations in both sexes in T. baccata (Iszkuło et al, 2009), similarly the taxol content depending on the age and sex of T. wallichiana from the Himalayan did not differ significantly between both the sexes (Nadeem et al, 2002). In contrast, Iszkuło et al (2013) found that female individuals of T. baccata exhibited a significantly higher level of taxanes.…”

Section: Introductionmentioning

confidence: 97%

Seasonal fluctuations of photosynthetic pigments content in Taxus baccata needles

Zarek¹

2016

Dendrobiology

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Yew is a coniferous, evergreen, dioecious species. The objective of the study was to present a comprehensive characteristic of changes occurring throughout the year in terms of the content of photosynthetic pigments and related compounds in the needles of yew, depending on the sex of individuals and age of needles. Eight compounds, particularly chlorophyll a (Chl a), chlorophyll b (Chl b), carotenoids (Car), protoporphyrin IX (PPIX), magnesium protoporphyrin IX (MgPPIX), protochlorophyllide (Pchlide), chlorophyllide a (Chlide a), and chlorophyllide b (Chlide b), were subjected to quantitative analysis. Based on the several parameters under study, significant differences between male and female individuals were observed, while most commonly, the largest differences were reported in the autumn and winter period. They were related to the content of Chl a, Chl b and Chl/Car ratio. The remaining compounds showed no significant differences according to the sex and were slightly different only in single periods. For all the studied parameters except for Chl/Car ratio, interaction between sampling dates and sex was not statistically significant. Significant differences between the needles of different age were observed only in terms of the content of Chl b, MgPPIX, Pchlide, Chlide b, and Chl a/b ratio, and these differences were always caused by the current-year needles.

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Taxol content in the bark of Himalayan Yew in relation to tree age and sex

Cited by 83 publications

References 20 publications

Enhancement of taxol production from endophytic fungus Fusarium redolens

Enhancement of taxol production from endophytic fungus Fusarium redolens

Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Seasonal fluctuations of photosynthetic pigments content in Taxus baccata needles

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