Taxonomic diversity of sputum microbiome in lung cancer patients and its relationship with chromosomal aberrations in blood lymphocytes

Дружинин, В. Г.; Matskova, Liudmila; Деменков, П. С.; Baranova, E. D.; Volobaev, Valentin P.; Минина, В. И.; Apalko, Svetlana V.; Churina, M. A.; Romanyuk, Sofya A.; Щербак, С. Г.; Иванов, В А; Ларионов, А. В.

doi:10.1038/s41598-020-66654-x

Cited by 27 publications

(25 citation statements)

References 43 publications

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“…Furthermore, a study showed that Haemophilus was more abundant in patients with lung cancer than in healthy individuals. [33] Our result showed that Haemophilus was abundant in the non-responder group; therefore, it can be inferred that members of this genus lower the effectiveness of anticancer drugs. Thus, Haemophilus may be considered as a target for disease-modulating drugs in lung cancer treatment.…”

Section: Discussionmentioning

confidence: 59%

Relationship of The Lung Microbiome With PD-L1 Expression And Immunotherapy Response in Lung Cancer

Jang

Choi

Kim

et al. 2021

Preprint

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Background: Lung cancer is the primary cause of cancer-related deaths worldwide. The human lung serves as a niche to a unique and dynamic bacterial community that is related to the development of multiple diseases. Here, we investigated the differences in the lung microbiomes of patients with lung cancer. Methods: 16S rRNA sequencing was performed to evaluate the respiratory tract microbiome present in the bronchoalveolar lavage fluid. Patients were stratified based on programmed death-ligand 1 (PD-L1) expression levels and immunotherapy responses. Results: In total, 84 patients were prospectively analyzed, of which 59 showed low (<10%), and 25 showed high (>10%) PD-L1 expression levels. The alpha and beta diversities did not significantly differ between the two groups. Veillonella dispar was dominant in the high-PD-L1 group; the population of Neisseria was significantly higher in the low-PD-L1 group than in the high-PD-L1 group. In the immunotherapy responder group, V. dispar was dominant, while Haemophilus influenzae and Neisseria perflava were dominant in the non-responder group. Conclusion: The abundances of Neisseria and V. dispar differed significantly in relation to PD-L1 expression levels and immunotherapy responses. Thus, these two genera may be considered targets for lung cancer immunotherapy.

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Section: Discussionmentioning

confidence: 59%

Relationship of The Lung Microbiome With PD-L1 Expression And Immunotherapy Response in Lung Cancer

Jang

Choi

Kim

et al. 2021

Preprint

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“…Growing pieces of evidence suggested that the development of cancer is affected by human commensal microbiota through inflammation, immunity, metabolism pathways [29]. Recently, various studies identified the alteration of airway microbiota among NSCLC patients [5,7,9,11,[30][31][32][33][34]. The interplay between microbiota and lung cancer is complex.…”

Section: Discussionmentioning

confidence: 99%

The Airway Microbiota of Non-small-cell Lung Cancer Patients and its Relationship to Tumor Stage and EGFR Gene Mutation

Huang

et al. 2021

Preprint

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Background: Accumulating studies have suggested the airway microbiota of lung cancer was significantly different from healthy controls. However, little was known about the relationship between airway microbiota and important clinical parameters of lung cancer. In this study, we aimed to explore the association between sputum microbiota and lung cancer stage, lymph node metastasis, intrathoracic metastasis, and Epidermal growth factor receptor (EGFR) gene mutation. Methods: The microbiota of sputum samples from 85 newly diagnosed NSCLC patients were sequenced via 16S rRNA sequencing with V3-V4 region. Sequencing reads were filtered using QIIME2 and clustered against UPARSE. Results: The α diversity and β diversity was significantly different between patients in stage I to II (early stage, ES) and patients in stage III to IV (advanced stage, AS). Lefse identified that genera Granulicatella and Actinobacillus were significantly enriched in ES, and genus Actinomyces were significantly enriched in AS. PICRUSt2 identified NAD salvage pathway was significantly enriched in AS, which was positively associated with Granulicatella. Patients with intrathoracic metastasis were associated with increased genus Peptostreptococcus and incomplete reductive TCA cycle. Enrichment of TCA cycle was associated with increased Peptostreptococcus. Genera Parvimonas, Pseudomona and L-valine biosynthesis were positively associated with lymph node metastasis. L-valine biosynthesis was related with increased Pseudomona. Finally, genus Parvimonas were significantly upregulated in adenocarcinoma patients with EGFR mutation. Conclusion: In conclusion, the taxonomy structure differed between different lung cancer stage. The tumor stage, intrathoracic 1 metastasis, lymph node metastasis, and EGFR mutation were associated with alteration of specific airway genera and metabolic function of sputum microbiota.

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“…There was a significant difference in the beta diversity of the sputum microbial composition, with genuses like Atopobium and Treponema decreasing significantly and Bergeyella increasing significantly in cancer patients. Reduction in species from genus Atopobium and increase in Alloprevotella species correlated with higher levels of chromosomal aberrations in sputum donors (175). On the other hand, oral administration of Lactobacillus johnsonii was able to alleviate lymphocytic as well as systemic genotoxicity in Atm −/− mice models of Ataxiatelangiectasia, thereby decreasing the development of lymphoma (176).…”

Section: Other Modes Of Gut Microbiome-immune Communicationmentioning

confidence: 94%

“…Microbial genotoxicity might play a role in anti-tumor immunity as well. Analysis of sputum samples from patients with lung cancer and healthy individuals showed an increased prevalence of leucocytes with chromosomal aberrations in patients with cancer (175). There was a significant difference in the beta diversity of the sputum microbial composition, with genuses like Atopobium and Treponema decreasing significantly and Bergeyella increasing significantly in cancer patients.…”

Section: Other Modes Of Gut Microbiome-immune Communicationmentioning

confidence: 97%

New Insights Into the Cancer–Microbiome–Immune Axis: Decrypting a Decade of Discoveries

et al. 2021

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The past decade has witnessed groundbreaking advances in the field of microbiome research. An area where immense implications of the microbiome have been demonstrated is tumor biology. The microbiome affects tumor initiation and progression through direct effects on the tumor cells and indirectly through manipulation of the immune system. It can also determine response to cancer therapies and predict disease progression and survival. Modulation of the microbiome can be harnessed to potentiate the efficacy of immunotherapies and decrease their toxicity. In this review, we comprehensively dissect recent evidence regarding the interaction of the microbiome and anti-tumor immune machinery and outline the critical questions which need to be addressed as we further explore this dynamic colloquy.

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Taxonomic diversity of sputum microbiome in lung cancer patients and its relationship with chromosomal aberrations in blood lymphocytes

Cited by 27 publications

References 43 publications

Relationship of The Lung Microbiome With PD-L1 Expression And Immunotherapy Response in Lung Cancer

Relationship of The Lung Microbiome With PD-L1 Expression And Immunotherapy Response in Lung Cancer

The Airway Microbiota of Non-small-cell Lung Cancer Patients and its Relationship to Tumor Stage and EGFR Gene Mutation

New Insights Into the Cancer–Microbiome–Immune Axis: Decrypting a Decade of Discoveries

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