Taxonomic Diversity of the Bacterial Community Associated with the Fruiting Bodies of the Myxomycete Lycogala epidendrum (L.) Fr.

Sizov, Lev R.; Лысак, Л. В.; Gmoshinskii, V. I.

doi:10.1134/s0026261721030115

Cited by 2 publications

(4 citation statements)

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“…It has long been believed that one or two bacterial species were closely associated with one plasmodium [ 6 , 22 ]; by using six myxomycetes plasmodia belonging to five genera and two families, our results illustrated that 38 to 52 bacterial OTUs were associated with one myxomycetes species, and in most cases, there were more than one dominant OTUs. Our results showed that plasmodia harbored much more abundant bacteria than previously thought; a similar result has been reported in the fruit body of Lycogala epidendrum [ 7 ]. These plasmodia-associated bacteria were predominately gram-negative (> 90%) and aerobic (> 99%) (Fig.…”

Section: Discussionsupporting

confidence: 90%

“…However, the culture-based methods may underestimate those non-cultivable bacteria, as shown in a study utilizing amplicon sequencing of 16S rRNA genes which found that 31 bacterial genera are inhabiting the fruiting bodies of the Lycogala epidendrum [ 7 ]. High-throughput sequencing provides an opportunity to better reveal the myxomycetes-associated bacteria diversity and may shed light on understanding the relationship between myxomycetes and bacteria.…”

Section: Introductionmentioning

confidence: 99%

“…However, the relationship between myxomycetes and bacteria may be more complex than mere predation [6]. The study of myxomycetes-associated bacteria has long attracted the attention of researchers for revealing ecological relationships between myxomycetes and bacteria, studying the contribution of myxomycetes to the formation of the environment's microbial communities [7], and understanding better of myxomycetes nutrition requirements. In myxomycetes' life cycle, swarm cells [8][9][10], myxamoeba [11][12][13], and plasmodia [14][15][16][17][18] are all associated with one or several certain species of bacteria based on the culture-based method [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

“…The study of myxomycetes-associated bacteria has long attracted the attention of researchers for revealing ecological relationships between myxomycetes and bacteria, studying the contribution of myxomycetes to the formation of the environment's microbial communities [7], and understanding better of myxomycetes nutrition requirements. In myxomycetes' life cycle, swarm cells [8][9][10], myxamoeba [11][12][13], and plasmodia [14][15][16][17][18] are all associated with one or several certain species of bacteria based on the culture-based method [6][7][8]. In view of statements that several species of bacteria were found inconstant association with different plasmodial species of myxomycetes and Acrasieae, a qualitative analysis of the microbial flora of gross cultures of plasmodia was undertaken to determine the significance of their association.…”

Section: Introductionmentioning

confidence: 99%

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Diversity of bacterial communities in the plasmodia of myxomycetes

et al. 2022

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Background Myxomycetes are a group of eukaryotes belonging to Amoebozoa, which are characterized by a distinctive life cycle, including the plasmodium stage and fruit body stage. Plasmodia are all found to be associated with bacteria. However, the information about bacteria diversity and composition in different plasmodia was limited. Therefore, this study aimed to investigate the bacterial diversity of plasmodia from different myxomycetes species and reveal the potential function of plasmodia-associated bacterial communities. Results The bacterial communities associated with the plasmodia of six myxomycetes (Didymium iridis, Didymium squamulosum, Diderma hemisphaericum, Lepidoderma tigrinum, Fuligo leviderma, and Physarum melleum) were identified by 16S rRNA amplicon sequencing. The six plasmodia harbored 38 to 52 bacterial operational taxonomic units (OTUs) that belonged to 7 phyla, 16 classes, 23 orders, 40 families, and 53 genera. The dominant phyla were Bacteroidetes, Firmicutes, and Proteobacteria. Most OTUs were shared among the six myxomycetes, while unique bacteria in each species only accounted for a tiny proportion of the total OTUs. Conclusions Although each of the six myxomycetes plasmodia had different bacterial community compositions, a high similarity was observed in the plasmodia-associated bacterial communities' functional composition. The high enrichment for gram-negative (> 90%) and aerobic (> 99%) bacteria in plasmodia suggest that myxomycetes may positively recruit certain kinds of bacteria from the surrounding environment.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Diversity of bacterial communities in the plasmodia of myxomycetes

et al. 2022

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Metagenomic Insight into the Associated Microbiome in Plasmodia of Myxomycetes

Peng,

Li,

Dou

et al. 2024

Microorganisms

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During the trophic period of myxomycetes, the plasmodia of myxomycetes can perform crawling feeding and phagocytosis of bacteria, fungi, and organic matter. Culture-based studies have suggested that plasmodia are associated with one or several species of bacteria; however, by amplicon sequencing, it was shown that up to 31–52 bacteria species could be detected in one myxomycete, suggesting that the bacterial diversity associated with myxomycetes was likely to be underestimated. To fill this gap and characterize myxomycetes’ microbiota and functional traits, the diversity and functional characteristics of microbiota associated with the plasmodia of six myxomycetes species were investigated by metagenomic sequencing. The results indicate that the plasmodia harbored diverse microbial communities, including eukaryotes, viruses, archaea, and the dominant bacteria. The associated microbiomes represented more than 22.27% of the plasmodia genome, suggesting that these microbes may not merely be parasitic or present as food but rather may play functional roles within the plasmodium. The six myxomycetes contained similar bacteria, but the bacteria community compositions in each myxomycete were species-specific. Functional analysis revealed a highly conserved microbial functional profile across the six plasmodia, suggesting they may serve a specific function for the myxomycetes. While the host-specific selection may shape the microbial community compositions within plasmodia, functional redundancy ensures functional stability across different myxomycetes.

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Taxonomic Diversity of the Bacterial Community Associated with the Fruiting Bodies of the Myxomycete Lycogala epidendrum (L.) Fr.

Cited by 2 publications

References 22 publications

Diversity of bacterial communities in the plasmodia of myxomycetes

Diversity of bacterial communities in the plasmodia of myxomycetes

Metagenomic Insight into the Associated Microbiome in Plasmodia of Myxomycetes

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