Taxonomic Position in the GenusBrucellaof the Causative Agent of Canine Abortion

Abstract

The gram-negative organism causing abortion in dogs was examined in parallel with cultures representative of the Brucella species and with Bordetella bronchisepfica. The organism fits into the genus Brucella and most closely resembles B. suis on the basis of its growth characteristics. It is of rough colonial morphology and is agglutinated by antisera prepared against rough Brucella. In mouse toxicity tests, no endotoxic activity could be demonstrated. In contrast to most Brucella cultures, it does not utilize erythritol. Electron microscopy showed a cell wall structure similar to that of other gram-negative organisms. The question of whether the organism should be designated Brucella canis, as proposed by Carmichael and Bruner, or Brucella suis biotype 5 is discussed. The authors favor the designation Brucella canis because the organism lacks the lipopolysaccharide antigen associated with the smooth agglutinogen and endotoxin, and it does not utilize erythritol.

Occurrence of Quinovosamine in Lipopolysaccharides of Brucella Species

“…IMMUNITY mon to the other smooth-type Brucellae. This result and interpretation support and extend observations that the serologically similar antigens of smooth type Brucellae are not found in B. canis (4,8,13).…”

Endotoxic activity of rough organisms of Brucella species

“…Earlier attempts to demonstrate endotoxin in heat-killed cells of non-S variants of B. abortus and the R species B. canis, or extracts from those cells, were unsuccessful (2,11,18,20). These limited studies have led to the generally held belief that endotoxin is associated only with S Brucellae (16), whereas R mutants of the Enterobacteriaceae are known to have endotoxin (13).…”

“…Lipopolysaccharides (LPS) extracted from smooth (S) Brucellae possess many structural and biological properties in common with the endotoxins of the Enterobacteriaceae (15). Although LPS (glycolipids) obtained from rough (R) mutants of the salmonellae are as toxic as LPS from smooth strains (13), there has been no evidence of endotoxin activity in whole cells or extracts from R strains of Brucellae (2,11,18), but the investigations have been limited in scope.…”

<b>Desenvolvimento, padronização e validação de reação em cadeia pela polimerase (PCR) em tempo real para diagnóstico da brucelose canina</b>

“…A brucelose é uma doença infecto-contagiosa crônica, de distribuição mundial causada por bactérias Gram-negativas, na forma de cocobacilos, intracelulares facultativas, não formadoras de endosporos, pertencentes à família Brucellaceae e gênero Brucella (JONES, 1968;BRUNNER, 1968;ALTON et al, 1976;CORBEL;BANAI, 2005).…”

“…Unidos da América (EUA), com isolamento de uma bactéria com características diversas das outras brucelas, mas bioquimicamente similar à B. suis, não produtora de H2S, produtora de urease, redutora de nitratos, oxidase positiva e não fermentadora, a qual foi denominada B. canis (ALTON et al, 1976;CARMICHAEL, 1966;BRUNNER, 1968;JONES et al, 1968).…”