Taxonomic Position of Anastrangalia reyi and A. sequensi (Coleoptera, Cerambycidae) Based on Molecular and Morphological Data

Abstract

Anastrangalia reyi (Heyden, 1889) and Anastrangalia sequensi (Reitter, 1898) are morphologically similar species described in late of XIX century. The recent barcoding revealed that A. reyi is almost identical to another species, Anastrangalia dubia (Scopoli, 1763), by the sequence of nucleotides in cytochrome C oxidase subunit I (COI). Consequently, the taxonomic position of these species is unclear. We have conducted a comprehensive meta-analysis of available data of COI sequences combined with a study of morphological characters of the male genitalia of A. reyi, A. sequensi and A. dubia. Based on 87 sequenced samples we built well-resolved phylogenetic maximum likelihood tree. We found the clades of A. dubia, A. reyi and A. sequensi to be closely related and arranged in the dense cluster. Despite this, numerous cases of introgressive hybridization of A. reyi and A. dubia were identified, indicating an inadequate reproductive barrier between them. The study of morphological features of male genitalia of A. reyi, A. sequensi and A. dubia shows minor differences between them. Based on these facts and the results of the phylogenetic analysis we propose to consider A. reyi and A. sequensi to be subspecies of A. dubia.

A survey of Lepturinae Latreille, 1802 (Coleoptera: Cerambycidae) of the south-eastern Baltic region (Lithuania and the Kaliningrad Region)

“…LT: Two taxons of the genus Anastrangalia (= Leptura = Anoplodera), A. dubia (Scopoli, 1763) and A. reyi (Heyden, 1889) = inexpectata (Jansson, Sjöberg, 1928) have been noted for Lithuania as a distinct species since 1960 (Pileckis, 1960). However, the taxonomic position of those, including Siberian A. sequensi (Reitter, 1898), was recently determined based on the analysis of sequences of nucleotides in cytochrome C oxidase I (COI) and all were considered to be subspecies of A. dubia (Zamoroka et al, 2019). Consequently, the previous faunistic data on the taxons from LT should be merged and related to the subspecies A. dubia reyi, because the territory of the region is outside the distribution range of the nominative subspecies A. dubia dubia (Scopoli) or subspecies Anastrangalia dubia sequensi (Reitter) and is within the range of the subspecies of A. dubia reyi only (Zamoroka et al, 2019).…”

“…However, the taxonomic position of those, including Siberian A. sequensi (Reitter, 1898), was recently determined based on the analysis of sequences of nucleotides in cytochrome C oxidase I (COI) and all were considered to be subspecies of A. dubia (Zamoroka et al, 2019). Consequently, the previous faunistic data on the taxons from LT should be merged and related to the subspecies A. dubia reyi, because the territory of the region is outside the distribution range of the nominative subspecies A. dubia dubia (Scopoli) or subspecies Anastrangalia dubia sequensi (Reitter) and is within the range of the subspecies of A. dubia reyi only (Zamoroka et al, 2019). The actual faunistic information related to this subspecies was given from Alytus, Kaunas (Ferenca, 2006), Lazdijai (Ostrauskas, Tamutis, 2012), Vilnius (Zawadzki, 1937 General distribution.…”

“…The actual faunistic information related to this subspecies was given from Alytus, Kaunas (Ferenca, 2006), Lazdijai (Ostrauskas, Tamutis, 2012), Vilnius (Zawadzki, 1937 General distribution. For this moment, the species is represented by five subspecies (Zamoroka et al, 2019;Danilevsky, 2019). The subspecies A. dubia reyi is widely distributed in northern and eastern Europe and is also known from the highlands of the Alps in isolated populations (Zamoroka et al, 2019) and northern Kazakhstan (Danilevsky, 2019).…”

“…The southern and westerns borders of its distribution range are not completely known and surely overlap with the range of the nominative subspecies in central Europe (Zamoroka et al, 2019) and with A. d. sequensi in West Siberia (Danilevsky, 2014). To the north, its distribution range goes as far as the border of the forest zone, up to 69°N in Finland and 65°N in the north-eastern part of European Russia.…”

Spatial patterns and habitat differentiation of the beetle (Insecta: Coleoptera) fauna of protected areas in the Eastern Carpathians (Ivano-Frankivsk Region, Ukraine)

“…Ecological studies of Coleoptera include altitudinal distribution (Rizun, Tymochko, Chumak, 2004), habitat patterns (Rizun, Tymochko, Chumak, 2004;Zamoroka, 2008), the influence of anthropogenic impact (Chumak et al, 2005), local extinction (Zamoroka et al, 2018;Zamoroka, 2019), etc. The phylogeographic coleopterological studies focused on certain species (Kajtoch, Lachowska-Cierlik, Mazur, 2009;Kubisz et al, 2012;Zamoroka et al, 2019). Despite the sufficiently well-known local fauna of Coleoptera, a number of faunal, ecological and phylogeographic aspects need to be clarified especially in the light of current climatic changes, ecosystems restoration, and conservation.…”