Taxonomic Position of Korean Isolates of Rhizoctonia solani Based on RAPD and ITS Sequencing of Ribosomal DNA

Abstract

Taxonomic position of 46 Korean isolates of Rhizoctonia solani which were classified into nine intraspecific groups by anastomosis and cultural characteristics was analyzed by randomly amplified polymorphic DNA (RAPD) and sequence analyses of the internal transcribed spacer (ITS) regions of ribosomal DNA. All the isolates within each group showed highly similar band patterns in RAPD. The ITS regions of the isolates within the same groups showed a high level of sequence similarity above 96.0% whereas similarities among different groups were below 94.4%. When compared with several reference strains of R. solani from foreign countries, all the Korean isolates were clustered with the foreign isolates belonging to the same groups in the phylogenetic tree. All six Korean strains of AG-4 were identified as HG-1 out of 3 subgroup of AG-4. We discussed taxonomic position of Korean isolates of R. solani and showed that sequence analysis with ITS regions could be a rapid and useful method for identification of intraspecific group of R. solani.

Identification of Trichoderma, a Competitor of Shiitake Mushroom (Lentinula edodes), and Competition between Lentinula edodes and Trichoderma species in Korea

“…NJ tree were constructed using PAUP version 4.0b10 (Swofford, 2003) using the Kimura 2-parameter model. Bootstrap support (BS) values for nodes were computed from 1000 replicates for NJ analyze (Jeon et al, 2010). The general time-reversible model, under the assumption of a discrete gammashaped rate variation with a proportion of invariable sites (GTR + I + G), was estimated as the best-fit likelihood model for the combined-sequences dataset.…”

Occurrence of Brown Patch on Kentucky Bluegrass Caused by Rhizoctonia solani AG-1 IB

“…Bars represent standard deviations. (Jeon et al, 2010). (Smiley, et al, 1992) 이후, 지 금까지 R. solani은 AG-1그룹으로 미국에서는 대부분이 tall fescue종에서 분리 동정 되었으며, R. solani AG2-2균 주는 난지형 잔디인 St. Augustinegrass의 줄기에서 분리되 었다 (Hurd and Grisham, 1983).…”

Chemical and Biological Controls of Balloon Flower Stem Rots Caused by Rhizoctonia solani and Sclerotinia sclerotiorum

“…were not identified so far requiring to be confirmed. R. solani infection led to disease occurrence in a wide range of plant species including monocots and dicots (Jeon et al, 2010). Different R. solani isolates originated from balloon flower plants grown in six locations of South Korea, showed differential pathogenicity in relation to their anastomosis and tissue specificity (Kim et al, 1992).…”