Taxonomic Significance of ISTR to Discriminate Species in Agavaceae

Review Article: ISTR, a Retrotransposons-Based Marker to Assess Plant Genome Variability with Special Emphasis in the Genera <i>Zea</i> and <i>Agave</i>

“…The amplification of ISTR patterns allowed differentiating between populations of A. durangensis. The ISTR fingerprinting allowed also clearly discriminating between the populations of A. durangensis and one population of Agave asperrima, the group used as reference, what is consistent with previous reports revealing the significance of the ISTR markers as valuable molecular tool at specific level in Agavaceae [24].…”

“…Ten primers of SSRs, one for each chromosome of maize and two combination of ISTR were used. PCR was carrying out according to Matzuoka et al [23] for SSRs and TorresMorán et al [24] for ISTRs. Molecular weight matrix was obtained from SSR data and was calculated the genetic distances from a correlation matrix.…”

“…In our study, we estimated the above mentioned population parameters, using the ISTR markers, in order to determine the specificity status of three natural populations of A. durangensis, since ISTR has been recognized as markers having a high capacity to quantify genetic diversity and a high specific discriminatory capability [21,24,[35][36][37]. The ISTR markers produced typical amplification profiles for each population of Agave durangensis, allowing distinguishing among them, which showed the capability of ISTR to discriminate among the natural populations of that taxon.…”

Assessment of genetic fidelity among Agave tequilana plants propagated asexually via rhizomes versus in vitro culture

“…The properties of this marker and its usefulness for classifying genetic differences among plants of the Agave genus have been described (Infante et al , 2007Torres-Morán et al 2008). Leaf samples were stored at -20°C for DNA isolation, which was performed using the method reported by Keb-Llanes et al (2002).…”

“…The inverse sequence-tagged repeat (ISTR) molecular marker has been reported to be an accurate tool for detecting genetic fidelity among species of this genus (Infante et al , 2007Torres-Morán et al 2008).…”