Taxonomic Significance of Phospholipids in Coryneform and Nocardioform Bacteria

Kōmura, Ichirō; Yamada, Katsushi; Ôtsuka, Shin-Ichirô; Komagata, Kazuo

doi:10.2323/jgam.21.251

Cited by 53 publications

(26 citation statements)

References 10 publications

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“…The present data showed that three species, Corynebacterium equi, Corynebacterium fascians, and Corynebacterium hoagii were glycolyl-type bacteria, and clearly separated from Corynebacterium diphtheriae (the type species of genus Corynebacterium or Group 1), as well as from all other strains of low-GC subgroup (Table 1). This result agrees apparently with the observation of KoMuRA et al (21). They reported that genus Corynebacterium could be divided into two groups by the phospholipid composition, and a large amount of phosphatidylethanolamine (PE) was contained only in strains with high GC-content, e.g., C. equi and C. fascians, although they have not extended their experiment over the other strains of high-GC subgroup, such as Brevibacteriurn linens AJ 1473, AJ 1475, AJ 1484, and Corynebacterium xerosis AJ 1375, AJ 1395, and AJ 1396.…”

Section: Resultssupporting

confidence: 94%

“…The pattern of cell-wall components in some actinomycete is summarized in Table 6. The differences between these genera of acetyl type and genus Nocardia have been confirmed by other chemotaxonomic studies showing absence of LCN-A (27) and PE (21) in the cell envelope of the former taxa, and the occurrence of specific menaquinone systems (40). Test …”

Section: Mycobacteriummentioning

confidence: 57%

“…They have a specific cell wall of glycolyl type and form a cluster in the high-GC subgroup of genus Corynebacterium (Group 1). The close relation between the abovementioned three species and Mycobacterium-Nocardia group should be supported by the similarity of other chemical constituents, namely, the GC content (19), the cell wall of Type IV (4,29), and presence of PE in their cell membranes (21). Some coryneform bacteria (e.g., B. imperiale, B. minutiferula, B. testaceum, and Mb.…”

Section: Mycobacteriummentioning

confidence: 91%

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Taxonomic Significance of Cell-Wall Acyl Type in Corynebacterium-Mycobacterium-Nocardia Group by a Glycolate Test

Uchida

Aida

1979

J. Gen. Appl. Microbiol.

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Section: Resultssupporting

confidence: 94%

Section: Mycobacteriummentioning

confidence: 57%

Section: Mycobacteriummentioning

confidence: 91%

See 1 more Smart Citation

Taxonomic Significance of Cell-Wall Acyl Type in Corynebacterium-Mycobacterium-Nocardia Group by a Glycolate Test

Uchida

Aida

1979

J. Gen. Appl. Microbiol.

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“…The phospholipid pattern (diphosphatidyl glycerol, phosphatidyl glycerol, phosphatidyl inositol, phosphatidylinositol mannoside, and seldom more than traces of phosphatidyl ethanolamine [43] is characteristic of corynebacteria related to the type species, C. diphtheriae, and the pattern in conjunction with the presence of corynomycolic acids, has been designated phospholipid fatty acid type 4 by Lechevalier et al…”

Section: Orla-jensen Noted Repeatedly In His Treatisementioning

confidence: 99%

Biological and Chemical Basis for the Reclassification of Microbacterium flavum Orla-Jensen as Corynebacterium flavescens nom. nov.

Barksdale

Lanéelle

Pollice

et al. 1979

International Journal of Systematic Bacteriology

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New Jersey 071 IdOn the basis of its biological properties (cell morphology, staining reactions, cellular inclusions, guanine plus cytosine content of deoxyribonucleic acid, degree of deoxyribonucleic acid homology with Corynebacterium diphtheriae strain PW8, and pattern of enzymic capacities) and chemical structures (peptidoglycan type, major cell wall polysaccharide, major phospholipids and glycolipid, as well as characteristic cell wall fatty acids), Microbacterium flavum Orla-Jensen (synonym: Myco bacterium flavum Jensen) is renamed Corynebacterium flavescens nom. nov. The specific epithet "flauum" has been changed to "flavescens" in order to avoid the creation of a later homonym of Corynebacterium flavum Kisskalt and Berend 1918. Strain 8 of Orla-Jensen (= ATCC 10340 = NCIB 8707) is herein designated the type strain of C. flavescens. An updated description of the organism is given. Microbacterium flavum Orla-Jensen 1919 (57)has long been recognized as being closely related to the group of microorganisms which comprise the Corynebacterium-Mycobacterium-Nocardia (11) group (38) of bacteria, first on morphological and cultural grounds, and more recently on the basis of cell wall structure (41,63,65,74) guanine plus cytosine (G+C) content of the deoxy-ribonucleic acid (DNA) (20,75), and generic lipids (30), and, herein, on the basis of characteristic lipids. We present here a further biological and chemical analysis of the strain herein proposed as the type strain of Microbacterium flauum and propose the transfer of this organism to the genus Corynebacterium. MATERIALS AND METHODSBacterial strains. M. flavum American Type Culture Collection (ATCC) strain 10340 (= National Collection of Industrial Bacteria [NCIB] strain 8707 = strain number 8 of Orla-Jensen [57]) was the only Microbacterium strain examined. Efforts to obtain other authentic strains of this species (62) failed. Some investigators who had recently worked with strains identified as M. flavum reported that their cultures were no longer viable (personal communications from K. Robinson [62] and Malcolm Woodbine, University of Nottingham School of Agriculture, Sutton Bonington, England). The Park-Williams number 8 strain of Corynebacterium diphtheriae (58), which we used as a source of "standard" DNA for the DNA hybridization studies, has recently been described in some detail (44). Other corynebacteria used as controls for various biochemical tests included: C. diphtheriae (mitis) strain C7 (14); C. diphtheriae (gravis) strain HF (10); C. pseudodiphtheriticum ATCC 10700, C. xerosis strain 53-K-1 (13); C. minutissimum ATCC 23348; C. renale ATCC 19412, NCTC 7449, and strain 8 from R. Yanagawa (34); C. pilosum strain 46 (76); C. cystitidis strains 42 and 47 (33, 76); C. genitalium strains 392-1 and 418-H (29); and Corynebacterium sp. strain 43 (isolated from a case of lepromatous leprosy). Additional control organisms included: C. pyogenes strain 6378 (13); Brevibacterium vitarumen strain 12143 (45); Brevibacterium liquefaciens ATCC 14929; Bacterionema...

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“…Recently, chemotaxonomy has been introduced for bacterial taxonomy and the analysis of lipids of the bacterial cell-wall and membrane has been much emphasized1-5, 7,8,[15][16][17][18][19]. The usefulness of GLC analysis on fatty acids for the classification and identification of bacteria has been well-document ed 1,4,5, [8][9][10][11][12][13][14][15][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Comparative Studies on Thin-Layer Chromatograms of Lipids from Various Phytopathogenic Bacteria.

Matsuyama

Mian

Akanda

et al. 1993

Jpn. J. Phytopathol.

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The direct colony TLC method was applied for the comparison of chromatographic profiles of lipids from phytopathogenic bacteria. One Ioopful bacterial colony was pasted directly on the silica gel TLC plate and dried completely. The first development with chloroform-methanol (2:1, v/v) for short time was conducted for 10 min. After air-drying, the pasted bacterial cells were scraped out and this plate was developed at same direction with chloroform-methanol-water (60:25:4, v/v/v), secondly. The spots were visualized by spraying with ninhydrin and successive heating. The chromatograms of the bacterial isolates were different at generic level and at species level in the cases of erwinia and pseudomonad. The spots appeared at the area from Rf 0.5-0.65 were reliable benchmarks for the differentiation of bacteria. Striking difference was observed between gram positive and negative bacteria. The major spots on the chromatograms of gram negative bacteria were absent on those of gram positive bacteria, Clavibacter spp. Well reproducibility of chromatogram was observed when the culture age and conditions of TLC were kept steadfastly.

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Taxonomic Significance of Phospholipids in Coryneform and Nocardioform Bacteria

Cited by 53 publications

References 10 publications

Taxonomic Significance of Cell-Wall Acyl Type in Corynebacterium-Mycobacterium-Nocardia Group by a Glycolate Test

Taxonomic Significance of Cell-Wall Acyl Type in Corynebacterium-Mycobacterium-Nocardia Group by a Glycolate Test

Biological and Chemical Basis for the Reclassification of Microbacterium flavum Orla-Jensen as Corynebacterium flavescens nom. nov.

Comparative Studies on Thin-Layer Chromatograms of Lipids from Various Phytopathogenic Bacteria.

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