Taxonomic significance of seed sculpture and pollen ecto-mycoflora at the infraspecific level: Brassica tournefortii case study

Hassan, Walaa A.; Abdel-Hameed, A.; Shaye, Najla A. Al; Amer, Wafaa M.

doi:10.1016/j.sjbs.2021.06.049

Cited by 1 publication

(1 citation statement)

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“…On this basis, the dominant species among our sites seems to have been B. rapa s.l., with representatives of B. nigra and B. napus also well represented. However, several studies have reported extensive intraspecific variability in the key diagnostic features of seed-surface architecture, seed size, and seed shape for species belonging to this genus [51][52][53][54]. Despite this known variability, none of the widely used diagnostic keys attempt to estimate the frequency of erroneous diagnosis [15,50,[55][56][57].…”

Section: Discussionmentioning

confidence: 99%

Brassica and Sinapis Seeds in Medieval Archaeological Sites: An Example of Multiproxy Analysis for Their Identification and Ethnobotanical Interpretation

Bosi

Felice

Wilkinson

et al. 2022

Plants

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The genus Brassica includes some of the most important vegetable and oil crops worldwide. Many Brassica seeds (which can show diagnostic characters useful for species identification) were recovered from two archaeological sites in northern Italy, dated from between the Middle Ages and the Renaissance. We tested the combined use of archaeobotanical keys, ancient DNA barcoding, and references to ancient herbarium specimens to address the issue of diagnostic uncertainty. An unequivocal conventional diagnosis was possible for much of the material recovered, with the samples dominated by five Brassica species and Sinapis. The analysis using ancient DNA was restricted to the seeds with a Brassica-type structure and deployed a variant of multiplexed tandem PCR. The quality of diagnosis strongly depended on the molecular locus used. Nevertheless, many seeds were diagnosed down to species level, in concordance with their morphological identification, using one primer set from the core barcode site (matK). The number of specimens found in the Renaissance herbaria was not high; Brassica nigra, which is of great ethnobotanical importance, was the most common taxon. Thus, the combined use of independent means of species identification is particularly important when studying the early use of closely related crops, such as Brassicaceae.

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Section: Discussionmentioning

confidence: 99%