Taxonomic study of some species of the subfamily Dipsacoideae Eaton (Caprifoliaceae) by phenolic acid profiles

Ebadi, Mostafa; Eftekharian, Rosa; Nikzat, Sedighe; Mollaei, Saeed

doi:10.14719/pst.2021.8.4.1270

Cited by 4 publications

(2 citation statements)

References 20 publications

(23 reference statements)

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“…The separation and identification of phenolic acids was done by HPLC 25 . The column used was C18 (250 mm × 0.46 mm, 5 μm).…”

Section: Methodsmentioning

confidence: 99%

Investigation the biological activities and the metabolite profiles of endophytic fungi isolated from Gundelia tournefortii L.

Ebadi,

Ahmadi,

Tahmouresi

et al. 2024

Sci Rep

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Endophytic fungi are microorganisms that are considered as a potential source of natural compounds, and can be applied in various industries. The aims of this research were molecular identification of endophytic fungi isolated from the Gundelia tournefortii stems, and investigation their biological activities as well as phenolic and fatty acid profile. Surface sterilized stems of G. tournefortii were placed on potato dextrose agar (PDA) to isolate the fungal endophytes. Genomic DNA was extracted by CTAB method, and PCR amplification was performed by ITS 1 and ITS 4 as primers. The enzyme production of endophytic fungi was determined based on the formation of a clear zone that appeared around the colonies of fungus. The anti-oxidant activity was evaluated by measuring the amount of free radicals DPPH. Also, the total phenol and flavonoid contents were measured obtained by Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. Moreover, the separation and identification of phenolic acids and fatty acids were done by HPLC and GC, respectively. Phylogenetic analysis was done based on the Internal Transcribed Spacer (ITS) region, and five isolates were identified as following: Aspergillus niger, Penicillium glabrum, Alternaria alternata, A. tenuissima, and Mucor circinelloides. Evaluation of the enzymatic properties showed that P. gabrum (31 ± 1.9 mm), and A. niger (23 ± 1.7) had more ability for producing pectinase and cellulase. The anti-oxidant activity of isolates showed that A. alternata extract (IC50 = 471 ± 29 µg/mL) had the highest anti-oxidant properties, followed by A. tenuissima extract (IC50 = 512 ± 19 µg/mL). Also, the extract of A. alternata had the greatest amount of total phenols and flavonoids contents (8.2 ± 0.4 mg GAL/g and 2.3 ± 0.3 mg QE/g, respectively). The quantification analysis of phenolic acid showed that rosmarinic acid, para-coumaric acid, and meta-coumaric acid (42.02 ± 1.31, 7.53 ± 0.19, 5.41 ± 0.21 mg/g, respectively) were the main phenolic acids in the studied fungi. The analysis of fatty acids confirmed that, in all fungi, the main fatty acids were stearic acid (27.9–35.2%), oleic acid (11.3–17.3%), palmitic acid (16.9–23.2%), linoleic acid (5.8–11.6%), and caprylic acid (6.3–10.9%). Our finding showed that endophytic fungi are a source of bioactive compounds, which could be used in various industries. This is the first report of endophytic fungi associated with G. tournefortii, which provides knowledge on their future use on biotechnological processes.

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“…The separation and identification of phenolic acids was done by HPLC 25 . The column used was C18 (250 mm × 0.46 mm, 5 μm).…”

Section: Methodsmentioning

confidence: 99%

Investigation the biological activities and the metabolite profiles of endophytic fungi isolated from Gundelia tournefortii L.

Ebadi,

Ahmadi,

Tahmouresi

et al. 2024

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…The separation and identi cation of phenolic acids was done by HPLC 25 . The column used was C18 (250 mm × 0.46 mm, 5 µm).…”

Section: Total Phenolic and Avonoid Contents Assaymentioning

confidence: 99%

Investigation the biological activities and the metabolite profiles of endophytic fungi isolated from Gundelia tournefortii L.

Ebadi,

Ahmadi,

Tahmouresi

et al. 2024

Preprint

Self Cite

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Endophytic fungi are microorganisms that are considered as a potential source of natural compounds, and can be applied in various industries. The aims of this research was to investigate the molecular identification, extracellular enzymetic activity as well as phenolic and fatty acid profile analysis, and antioxidant activities of endophytic fungi isolated from the Gundelia tournefortii stems. Phylogenetic analysis was done based on the Internal Transcribed Spacer (ITS) region, and five isolates were identified as following: Aspergillus niger, Penicillium glabrum, Alternaria alternata, A. tenuissima, and Mucor circinelloides. Evaluation of the enzymatic properties showed that P. gabrum and A. niger had more ability for producing most of the studied extracellular enzymes, unlike to A. alternata and M. circinelloides which only able for degradation of starch and pectin. The antioxidant activity of isolates showed that A. alternata extract (IC50= 417 µg/mL) had the highest antioxidant properties, followed by A. tenuissima extract (IC50= 512 µg/mL). Also, the extract of A. alternata had the greatest amount of total phenols and flavonoids contents (2.8 mg GAL/g and 2.3 mg QE/g, respectively). The quantification analysis of phenolic acid showed that rosmarinic acid, para-coumaric acid, and meta-coumaric acid (42.02, 7.53, 5.41 mg/g, respectively) were the main phenolic acids in the studied fungi. The analysis of fatty acids confirmed that, in all fungi, the main fatty acids were stearic acid (27.9-35.2%), oleic acid (11.3-17.3%), palmitic acid (16.9-23.2%), linoleic acid (5.8-11.6%), and caprylic acid (6.3-10.9%). Our finding showed that endophytic fungi are a source of bioactive compounds, which could be used in various industries. This is the first report of endophytic fungi associated with G. tournefortii, which provides knowledge on their future use on biotechnological processes.

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A new and highly efficient source of t-resveratrol: Cephalaria Syriaca (L.) Roem. & Schult.

Nuralın,

Taşdemir

2024

Food Chemistry

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Taxonomic study of some species of the subfamily Dipsacoideae Eaton (Caprifoliaceae) by phenolic acid profiles

Cited by 4 publications

References 20 publications

Investigation the biological activities and the metabolite profiles of endophytic fungi isolated from Gundelia tournefortii L.

Investigation the biological activities and the metabolite profiles of endophytic fungi isolated from Gundelia tournefortii L.

Investigation the biological activities and the metabolite profiles of endophytic fungi isolated from Gundelia tournefortii L.

A new and highly efficient source of t-resveratrol: Cephalaria Syriaca (L.) Roem. & Schult.

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