Taxonomic Study of the Lactobacillus acidophilus Group, with Recognition of Lactobacillus gallinarum sp. nov. and Lactobacillus johnsonii sp. nov. and Synonymy of Lactobacillus acidophilus Group A3 (Johnson et al. 1980) with the Type Strain of Lactobacillus amylovorus (Nakamura 1981)

Fujisawa, Tomohiko; Benno, Yoshimi; Yaeshima, Tomoko; Mitsuoka, Tomotari

doi:10.1099/00207713-42-3-487

Cited by 210 publications

(130 citation statements)

References 13 publications

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“…L. johnsonii and L. gasseri are described as being very closely related (27). This is supported by comparison of the 16S ribosomal genes (99.6% identical) and the observation that the DNA sequences of many housekeeping genes of these two bacteria also show a high degree of similarity.…”

Section: Resultssupporting

confidence: 57%

“…The genome sequence predicts L. johnsonii has a strict anaerobic energy metabolism fermenting a variety of disaccharides and hexoses to lactic acid, and perhaps some acetate via its Embden-Meyerhoff pathway. These sugars include some not previously recognized as L. johnsonii growth substrates: galactose, maltose, sorbose͞sorbitol, gentiobiose, isoprimerevose, isomaltose, and panose (27). In contrast to L. gasseri, NCC 533 has acetolactate synthase and ␣-acetolactate decarboxylase (alsS, LJ1124 and aldB, LJ1125, respectively), so it likely converts some pyruvate to acetoin.…”

Section: Resultsmentioning

confidence: 99%

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The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533

Pridmore

Berger

Desiere

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

456

416

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Lactobacillus johnsonii NCC 533 is a member of the acidophilus group of intestinal lactobacilli that has been extensively studied for their ''probiotic'' activities that include, pathogen inhibition, epithelial cell attachment, and immunomodulation. To gain insight into its physiology and identify genes potentially involved in interactions with the host, we sequenced and analyzed the 1.99-Mb genome of L. johnsonii NCC 533. Strikingly, the organism completely lacked genes encoding biosynthetic pathways for amino acids, purine nucleotides, and most cofactors. In apparent compensation, a remarkable number of uncommon and often duplicated amino acid permeases, peptidases, and phosphotransferase-type transporters were discovered, suggesting a strong dependency of NCC 533 on the host or other intestinal microbes to provide simple monomeric nutrients. Genome analysis also predicted an abundance (>12) of large and unusual cell-surface proteins, including fimbrial subunits, which may be involved in adhesion to glycoproteins or other components of mucin, a characteristic expected to affect persistence in the gastrointestinal tract (GIT). Three bile salt hydrolases and two bile acid transporters, proteins apparently critical for GIT survival, were also detected. In silico genome comparisons with the >95% complete genome sequence of the closely related Lactobacillus gasseri revealed extensive synteny punctuated by clear-cut insertions or deletions of single genes or operons. Many of these regions of difference appear to encode metabolic or structural components that could affect the organisms competitiveness or interactions with the GIT ecosystem.

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Section: Resultssupporting

confidence: 57%

Section: Resultsmentioning

confidence: 99%

The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533

Pridmore

Berger

Desiere

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

456

416

View full text Add to dashboard Cite

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“…We included two different subcultures of the type strain of L. gasseri (Table 1) and found them both to belong to the subcluster B 1 (mean correlation r = 0.96 ; Fig. 1 DNA similarity group B2 was recently named L. johnsonii (Fujisawa et al, 1992). The proposed type strain (ATCC 33200) was identical to strain LMG 9436T, which was obtained from the National Collection of Food Bacteria (Reading, UK) as NCFB 2241T.…”

Section: Resultsmentioning

confidence: 99%

“…The heterogeneity was also reflected by the results of DNA : DNA hybridization studies (Johnson et al, 1980;Lauer et al, 1980). L. acidophilus and L. gasseri are found in similar habitats and cannot be distinguished by simple phenotypic criteria (Kandler & Weiss, 1986 L. acidophilus DNA similarity groups A4 and B2, respectively (Fujisawa et al, 1992). In this paper, we have applied SDS-PAGE, rRNA sequence analysis and oligonucleotide probe hybridization to study the relationships in the heterogeneous L. acidophilus complex.…”

Section: Introductionmentioning

confidence: 99%

Identification and Classification of Lactobacillus Acidophilus, L. Gasseri and L. Johnsonii Strains by SDS-PAGE and rRNA-Targeted Oligonucleotide Probe Hybridization

Pot

Hertel

Ludwig

et al. 1993

Journal of General Microbiology

143

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Thirty-two strains originally identified as Lactobacillus acidophilus and L. gasseri were screened for their taxonomic homogeneity by SDS-PAGE of whole-cell proteins. After numerical comparison of the resulting protein electrophoretic fingerprints, two well-delineated clusters were detected. The majority of the strains grouped in one electrophoretic cluster, which contained the type strain of L. acidophilus and corresponds to DNA group A1 of Johnson, J. L., Phelps, C. F., Cummins, C. S., London, J. & Gasser, F. (1980; Znternational Journalof Systematic Bacteriology 30, 53-68). Another cluster corresponded to DNA group B. It contained two subclusters, which agreed perfectly with DNA subgroups B l (L. gasseri) and B2 (A. johnsonii), respectively. The 23s rRNA genes were partially sequenced and 23s-rRNA-targeted oligonucleotide probes were designed for identification of DNA groups Al, B1 and B2. Probe Lbg reacted with all strains of electrophoretic cluster B1 (L. gasseri), probe Lbj hybridized with strains of cluster B2 (L. johnsonii) and probe Lba with strains of cluster A1 (authentic L. acidophilus). The probes were successfully used for the identification of strains belonging to the respective species. The phylogenetic relationship of a representative of L. johnsonii was determined by comparative sequence analysis of the 16s rRNA genes. It is very closely related to L. gasseri.

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“…This group of bacteria, including L. gasseri, which is found in the human intestine, has been classified on the basis of similarities in rRNA sequences and compositions of the cell walls into six subgroups (9,11,18). Many strains in this group have been shown to be bacteriocin producers (6,12,19,23).…”

mentioning

confidence: 99%

Structural and Functional Differences in Two Cyclic Bacteriocins with the Same Sequences Produced by Lactobacilli

Kawai

Ishii

Arakawa

et al. 2004

Appl Environ Microbiol

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Lactobacillus gasseri LA39 and L. reuteri LA6 isolated from feces of the same human infant were found to produce similar cyclic bacteriocins (named gassericin A and reutericin 6, respectively) that cannot be distinguished by molecular weights or primary amino acid sequences. However, reutericin 6 has a narrower spectrum than gassericin A. In this study, gassericin A inhibited the growth of L. reuteri LA6, but reutericin 6 did not inhibit the growth of L. gasseri LA39. Both bacteriocins caused potassium ion efflux from indicator cells and liposomes, but the amounts of efflux and patterns of action were different. Although circular dichroism spectra of purified bacteriocins revealed that both antibacterial peptides are composed mainly of ␣-helices, the spectra of the bacteriocins did not coincide. The results of D-and L-amino acid composition analysis showed that two residues and one residue of D-Ala were detected among 18 Ala residues of gassericin A and reutericin 6, respectively. These findings suggest that the different D-alanine contents of the bacteriocins may cause the differences in modes of action, amounts of potassium ion efflux, and secondary structures. This is the first report that characteristics of native bacteriocins produced by wild lactobacillus strains having the same structural genes are influenced by a difference in D-amino acid contents in the molecules.

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Taxonomic Study of the Lactobacillus acidophilus Group, with Recognition of Lactobacillus gallinarum sp. nov. and Lactobacillus johnsonii sp. nov. and Synonymy of Lactobacillus acidophilus Group A3 (Johnson et al. 1980) with the Type Strain of Lactobacillus amylovorus (Nakamura 1981)

Cited by 210 publications

References 13 publications

The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533

The genome sequence of the probiotic intestinal bacterium Lactobacillus johnsonii NCC 533

Identification and Classification of Lactobacillus Acidophilus, L. Gasseri and L. Johnsonii Strains by SDS-PAGE and rRNA-Targeted Oligonucleotide Probe Hybridization

Structural and Functional Differences in Two Cyclic Bacteriocins with the Same Sequences Produced by Lactobacilli

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