Taxonomy and geographical distribution of Dugesia japonica and D. ryukyuensis in the Far East

Kawakatsu, Masaharu; Oki, Iwashiro; Tamura, Shinji

doi:10.1007/bf00036363

Cited by 30 publications

(11 citation statements)

References 6 publications

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“…In view of the fact that, in most of our molecular analyses and particularly in the tree based on the concatenated dataset ( Fig. 1), D. umbonata was the sister-species of D. japonica and that the latter occurs also in China (Kawakatsu et al 1995), it is opportune to discuss the similarities and differences between these two species in some more detail. In particular, it is noteworthy that specimens of D. japonica have been described in which the vaginal region of the bursal canal is surrounded by a "…very wide halo-like structure…[consisting of] …mesenchymal tissue traversed by several coarse rows of longitudinal muscles and less-developed radial ones" (Kawakatsu et al 1976: p. 85).…”

Section: Discussionmentioning

confidence: 99%

A new species of Dugesia (Platyhelminthes, Tricladida, Dugesiidae) from China, with an account on the histochemical structure of its major nervous system

Song¹,

Li²,

Sluys³

et al. 2020

ZSE

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By means of an integrated approach, including molecular, morphological, anatomical and histological data, we describe a new species of freshwater flatworm of the genus Dugesia from southwest China, representing the third species recorded for the country. Morphologically, the new species, Dugesia umbonata Song & Wang, sp. nov., is particularly characterised by the presence of a muscularised hump immediately antero-dorsally to a knee-shaped bend in its bursal canal and by an ejaculatory duct that opens subterminally through the dorsal side of the penis papilla. Four molecular datasets (18S rDNA; ITS-1; 28S rDNA; COI) facilitated determination of the phylogenetic position of the new species, which belongs to a clade comprising other species from the Australasian and Oriental regions. We also analysed the structure of its major nervous system by means of the acetylcholinesterase (AChE) histochemical method and compared these results with data available for three other species of Dugesia.

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Section: Discussionmentioning

confidence: 99%

A new species of Dugesia (Platyhelminthes, Tricladida, Dugesiidae) from China, with an account on the histochemical structure of its major nervous system

Song¹,

Li²,

Sluys³

et al. 2020

ZSE

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“…The OH strain of the planarian D. ryukyuensis (Kawakatsu et al 1976(Kawakatsu et al , 1995 was donated by Dr S. Ishida of Hirosaki University. The worms in the OH strain (OH worms) were used as the wild-type strain.…”

Section: Animalsmentioning

confidence: 99%

Neoblast‐enriched fraction rescues eye formation in eye‐defective planarian ‘menashi’Dugesia ryukyuensis

et al. 2008

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Planarians are well known for their remarkable regenerative capacity. This capacity to regenerate is thought to be due to the presence of totipotent somatic stem cells known as 'neoblasts', which have particular morphological characteristics. The totipotency of neoblasts was supported by Baguñà's experiment, which involved the introduction of donor cells into irradiated hosts. However, since Baguñà's experiment did not include the use of a phenotypic marker, the donor cells could not be traced. In the current study, a genetic mutant planarian, menashi, an eye-defective mutant that lacks the pigmented area in the eyes, was established. This planarian is excellent for tracing the fate of cells after their introduction into irradiated hosts. To investigate the differentiation potency more directly, a neoblast-rich fraction obtained from normal worms was transplanted into an X-ray-irradiated menashi strain. Planarians that survive X-ray irradiation were developed, and we observed the pigment of the area in the eyes of the regenerating planarians. This result suggests that the neoblast-rich fraction contains cells that can proliferate and differentiate. These cells can replace the cells and structures lost by X-ray irradiation and ablation, and they can also differentiate into eye pigment cells.

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“…Preliminary Utilization of ECM-body as Three-Dimensional Scaffold -To explore the potential of utilizing the ECM body as a biological 3D scaffold for replanting of the neoblasts, we shifted our attention to a well-established helminth model, Dugesia japonica Due to the availability of genomic information and an established protocol for FACS sorting of neoblast (23). Without any modification, the intact ECM-body from D. japonica can be similarly prepared using our established procedure ( Fig 3B-C, E).…”

Section: Characterization Of Acellular Planarian Ecm-body -As One Of mentioning

confidence: 99%

ECM-body: A cell-free 3D biomimetic scaffold derived from intact planarian body

Sonpho

Wootthichairangsan

Ishida

et al. 2019

Preprint

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Extracellular matrix (ECM) plays key roles in shaping fates of stem cells, not only by providing suitable niche but also by mediating physical and biochemical cues. Despite intensive investigations on regeneration, the roles of ECM on fate determination of stem cells in animal with great regenerative potency, such as planarian, remained unclear. Here, we developed a method to decellularizing and isolating extracellular matrix from planarians. Although the isolated scaffold appears translucent, it contains all the internal features, resembling the structure of intact planarian, and which we thus called "ECM-body". Nuclear staining demonstrated that ECM-body contains very little or no cell remained. Histological sections displayed a well-preserved morphological integrity of the specimen. Scanning electron microscope showed porous surface on ECM-body, potentially suitable for housing cells.Furthermore, our preliminary experiment suggested that ECM-body can be utilized as biomimetic scaffold for cell culture as it may support survival of injected neoblasts.

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Taxonomy and geographical distribution of Dugesia japonica and D. ryukyuensis in the Far East

Cited by 30 publications

References 6 publications

A new species of Dugesia (Platyhelminthes, Tricladida, Dugesiidae) from China, with an account on the histochemical structure of its major nervous system

A new species of Dugesia (Platyhelminthes, Tricladida, Dugesiidae) from China, with an account on the histochemical structure of its major nervous system

Neoblast‐enriched fraction rescues eye formation in eye‐defective planarian ‘menashi’Dugesia ryukyuensis

ECM-body: A cell-free 3D biomimetic scaffold derived from intact planarian body

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