Coccolithophores are an ideal test group for investigating fine-scale differentiation within the phytoplankton since their taxonomy is rather well-documented and their biomineralised periplasts -the coccoliths -provide a rich suite of qualitative and quantitative morphological characters and a uniquely extensive fossil record. In addition, extant coccolithophore species can be grown in culture and hence are available for studies of morphological variability under controlled conditions, molecular genetic studies and cytological research.For the CODENET project the following extant species with seemingly global occurrence and spanning the biodiversity of coccolithophores were selected: Coccolithus pelagicus, Calcidiscus leptoporus, Umbilicosphaera sibogae, Syracosphaera pulchra, Gephyrocapsa oceanica, and Helicosphaera carteri. These were used as case studies to elucidate their species level biodiversity. The key merit of our approach was the use of multiple, independent lines of evidence, so as to remove the ambiguity implicit in any one type of study. In each case morphotypes of previously uncertain significance have been shown to represent discrete genotypes and probably well-separated species, with divergence times ranging 314 Geisen et al. from about 100 kyrs to >10 myrs. The evolutionary significance of these results is discussed.