Taxonomy and Multigene Phylogeny of Diaporthales in Guizhou Province, China

Abstract

In a study of fungi isolated from plant material in Guizhou Province, China, we identified 23 strains of Diaporthales belonging to nine species. These are identified from multigene phylogenetic analyses of ITS, LSU, rpb2, tef1, and tub2 gene sequence data coupled with morphological studies. The fungi include a new genus (Pseudomastigosporella) in Foliocryphiaceae isolated from Acer palmatum and Hypericum patulum, a new species of Chrysofolia isolated from Coriaria nepalensis, and five new species of Diaporthe isolated from Juglans regia, Eucommia ulmoides, and Hypericum patulum. Gnomoniopsis rosae and Coniella quercicola are newly recorded species for China.

Phylogenetic and Morphological Evidence for Three New Species of Diaporthales (Ascomycota) from Fujian Province, China

“…The Diaporthales constitute a cosmopolitan, highly diverse group of sac fungi that includes saprophytic, endophytic, and phytopathogenic members; however, important aspects of their interfamilial taxonomic relationships remain poorly characterized. A number of Diaporthales species were resolved based on morphological characteristics and multi-locus phylogenetic analyses [ 15 ]. In this study, three new species ( Chrysofolia camelliae sp.…”

“…Fungal isolates were single colonies purified by culturing on potato dextrose agar (PDA) in a light incubator at 25 °C and under a 12 h light/dark regime. To promote sporulation, some strains were specially removed to synthetic low nutrient agar (SNA) containing sterilized pine needles [ 15 ]. Dried holotype specimens were deposited in the Herbarium Mycologicum Academiae Sinicae, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China (HMAS).…”

“…(Cryphonectriaceae) was erected based on the type species C. colombiana by Crous et al [ 13 ]. Chrysofolia is mainly characterized by separate-to-aggregated pycnidial conidiomata with globose pycnidia [ 13 , 14 ], conidiophores reduced to conidiogenous cells [ 15 ], hyaline, smooth, aggregated, and cylindrical-to-ampulliform conidiogenous cells [ 13 , 16 ], and possessing conidia with hyaline, smooth, guttulate, straight-to-allantoid base with flattened hilum. Species within Chrysofolia have been reported to inhabit or colonize plant tissues as endophytes and pathogens [ 16 ].…”

“…Species within Chrysofolia have been reported to inhabit or colonize plant tissues as endophytes and pathogens [ 16 ]. Currently, Chrysofolia includes six species: C. barringtoniae [ 16 ], C. colombiana [ 13 ], C. coriariae [ 15 ], C. fructicola [ 17 ], C. galloides [ 18 ], and C. kunmingensis [ 14 ].…”