Taxonomy and phylogenetic appraisal of Spegazzinia musae sp. nov. and S. deightonii (Didymosphaeriaceae, Pleosporales) on Musaceae from Thailand

Samarakoon, Binu C.; Phookamsak, Rungtiwa; Wanasinghe, Dhanushka N.; Chomnunti, Putarak; Hyde, Kevin D.; McKenzie, Eric H. C.; Promputtha, Itthayakorn; Xu, Jianchu; Li, Yunju

doi:10.3897/mycokeys.70.52043

Cited by 20 publications

(15 citation statements)

References 58 publications

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“…Spegazzenia is a wood saprotroph with no endophytic capability, as described in Table 4 , obtained from FungalTraits. This genus of Spegazzenia was observed by Samarakoon et al [ 63 ] to be associated with Musa sp. (banana).…”

Section: Discussionmentioning

confidence: 93%

Applying EDTA in Chelating Excess Metal Ions to Improve Downstream DNA Recovery from Mine Tailings for Long-Read Amplicon Sequencing of Acidophilic Fungi Communities

Nkuna

Ijoma

Matambo

2022

JoF

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The hostile environment of mine tailings contains unique microbial life capable of bioleaching. The metagenomic analysis of such an environment provides an in-depth understanding of the microbial life and its potential, especially in biomining operations. However, DNA recovery from samples collected in those environments is challenging due to the presence of metal ions that interfere with the DNA analysis. A varied concentration of EDTA (4–13 µg/µL) to chelate the metal ions of enriched tailing samples prior to DNA extraction was performed. The results show that 9 µg/µL of EDTA was effective in most samples. However, the increasing concentration of EDTA negatively affected the DNA recovery. The sequencing of the successfully extracted DNA revealed a diverse range of fungal genera, some of which have not been previously reported in tailing or bioleaching applications. The dominant genera include Fodinomyces, Penicillium, Recurvomuces, Trichoderma, and Xenoacremonium; their traits were determined using the FungalTraits database. This study demonstrates the need to include a preliminary metal-chelating step using EDTA before DNA extractions for samples collected from metal-rich environments. It further showed the need for optimization but provided a benchmark range, particularly for tailings. However, we caution that a further EDTA removal step from the extracted DNA should be included to avoid its interferences in downstream applications.

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Section: Discussionmentioning

confidence: 93%

Applying EDTA in Chelating Excess Metal Ions to Improve Downstream DNA Recovery from Mine Tailings for Long-Read Amplicon Sequencing of Acidophilic Fungi Communities

Nkuna

Ijoma

Matambo

2022

JoF

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“…Each newly generated sequence was assembled using BioEdit 7.0.9.0 [ 51 ] and subjected to BLAST searches against the NCBI nucleotide non-redundant database ( (accessed on 20 August 2021)) for selection of the closest matching taxa. Based on BLAST search results and recently published data, sequences of representative taxa were downloaded and used for comparison [ 30 , 33 , 34 , 52 ] ( Table 1 ). Individual gene regions were aligned using MAFFT v.7 ( (accessed on 20 May 2022)) [ 53 ], and the uninformative gaps and ambiguous regions were manually removed and different gene regions were concatenated using BioEdit 7.0.9.0.…”

Section: Methodsmentioning

confidence: 99%

Taxonomic Novelties of Woody Litter Fungi (Didymosphaeriaceae, Pleosporales) from the Greater Mekong Subregion

Ren

Wanasinghe

Farias

et al. 2022

Biology

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The Greater Mekong Subregion (GMS) is known as a diverse geographic landscape and one of the richest biodiversity hotspots in the world with a high fungal diversity. Collections were carried out in terrestrial habitats to determine the diversity of woody litter fungi in the GMS, with an emphasis on northern Thailand and the Yunnan Province of China. Morphological characteristics and multigene phylogenetic analyses of combined SSU, LSU, ITS, and tef1-α supported the placement of the new isolates in the family Didymosphaeriaceae. The phylogenetic affinities of our isolates are illustrated through maximum likelihood and Bayesian inference analyses. Seven species of woody litter fungi were identified, comprising a new monotypic genus, Septofusispora; five novel species (Chromolaenicola sapindi, Dictyoarthrinium thailandicum, Karstenula lancangensis, Septofusispora thailandica, and Spegazzinia jinghaensis); and new host records of two species (Austropleospora archidendri, and Montagnula donacina). Furthermore, this study provides a synopsis of the Montagnula aff. donacina species based on their morphological characteristics, which can be useful in the species-level identifications in this genus.

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“…The reaction was conducted by running for 40 cycles. The annealing temperatures followed Lombard et al [ 20 ] and Samarakoon et al [ 14 , 59 ]. The amplified PCR fragments were sent to a commercial sequencing provider (TsingKe Biological Technology Co., Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…In the past three decades, there have been several studies on saprobic fungi associated with tropical monocotyledonous hosts [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 ]. In addition, detailed taxonomic studies have been conducted to describe and document the saprobic fungi on Musa across South and South East Asia [ 11 , 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Stachybotrys musae sp. nov., S. microsporus, and Memnoniella levispora (Stachybotryaceae, Hypocreales) Found on Bananas in China and Thailand

Samarakoon

Wanasinghe

Phookamsak

et al. 2021

Life

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A study was conducted to investigate saprobic fungal niches of Stachybotryaceae (Hypocreales) associated with leaves of Musa (banana) in China and Thailand. Three hyphomycetous taxa were collected during the dry season of 2018 and 2019. After a careful phenotypic characterization (both macro- and microscopically) and a phylogenetic tree reconstruction using a concatenated sequence dataset of internal transcribed spacer (ITS), calmodulin (cmdA), RNA polymerase II second largest subunit (rpb2), β-tubulin (tub2), and the translation elongation factor 1-alpha (tef1) gene regions, we report three species of Stachybotryaceae. Stachybotrys musae is introduced as a novel taxon from Yunnan, China, while S.microsporus is reported from Chiang Rai Province in Thailand on Musa. In addition, Memnoniella levispora is also reported from China for the first time.

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Taxonomy and phylogenetic appraisal of Spegazzinia musae sp. nov. and S. deightonii (Didymosphaeriaceae, Pleosporales) on Musaceae from Thailand

Cited by 20 publications

References 58 publications

Applying EDTA in Chelating Excess Metal Ions to Improve Downstream DNA Recovery from Mine Tailings for Long-Read Amplicon Sequencing of Acidophilic Fungi Communities

Applying EDTA in Chelating Excess Metal Ions to Improve Downstream DNA Recovery from Mine Tailings for Long-Read Amplicon Sequencing of Acidophilic Fungi Communities

Taxonomic Novelties of Woody Litter Fungi (Didymosphaeriaceae, Pleosporales) from the Greater Mekong Subregion

Stachybotrys musae sp. nov., S. microsporus, and Memnoniella levispora (Stachybotryaceae, Hypocreales) Found on Bananas in China and Thailand

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