Taxonomy and phylogeny of hyaline-spored coelomycetes

Li, Wenjing; McKenzie, Eric H. C.; Liu, Jian‐Kui; Bhat, D. Jayarama; Dai, Dong-Qin; Camporesi, Erio; Tian, Qing; Maharachchikumbura, Sajeewa S. N.; Luo, Zong-Long; Shang, Qiu-Ju; Zhang, Jinfeng; Tangthirasunun, Narumon; Karunarathna, Samantha C.; Xu, Jianchu; Hyde, Kevin D.

doi:10.1007/s13225-020-00440-y

Cited by 70 publications

(58 citation statements)

References 548 publications

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“…Morphologically, C. verrucosa has similar characteristics to C. friesii , but it can be identified by having verrucosa symptoms in branches and pycnidia with a central column, and having numerous ostioles on a large area of the ectostromatic disc. Moreover, C. verrucosa differs from the closest species C. globosa by larger size of conidia (6.5–8 × 1.5–2 vs. 4–6.5 × 1–2 μm) (Li et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Assessment of Cytospora Isolates From Conifer Cankers in China, With the Descriptions of Four New Cytospora Species

et al. 2021

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Cytospora species are widely distributed and often occur as endophytes, saprobes or phytopathogens. They primarily cause canker and dieback diseases of woody host plants, leading to the growth weakness or death of host plants, thereby causing significant economic and ecological losses. In order to reveal the diversity of Cytospora species associated with canker and dieback diseases of coniferous trees in China, we assessed 11 Cytospora spp. represented by 28 fungal strains from symptomatic branches or twigs of coniferous trees, i.e., Juniperus procumbens, J. przewalskii, Picea crassifolia, Pinus armandii, P. bungeana, Platycladus orientalis in China. Through morphological observations and multilocus phylogeny of ITS, LSU, act, rpb2, tef1-α, and tub2 gene sequences, we focused on four novel Cytospora species (C. albodisca, C. discostoma, C. donglingensis, and C. verrucosa) associated with Platycladus orientalis. This study represented the first attempt to clarify the taxonomy of Cytospora species associated with canker and dieback symptoms of coniferous trees in China.

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Section: Resultsmentioning

confidence: 99%

Assessment of Cytospora Isolates From Conifer Cankers in China, With the Descriptions of Four New Cytospora Species

et al. 2021

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“…The AJOM fungal notes series publishes new collections of known species with sequence data (Hyde et al 2020 ) in a novel format to emphasize the importance of such contributions. The data with imagery is also placed online in websites developed for specific groups (Jayawardena et al 2019 ; Pem et al 2019 ; Li et al 2020b ).…”

Section: Species: From Concepts To Identificationmentioning

confidence: 99%

Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?

et al. 2020

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True fungi (Fungi) and fungus-like organisms (e.g. Mycetozoa, Oomycota) constitute the second largest group of organisms based on global richness estimates, with around 3 million predicted species. Compared to plants and animals, fungi have simple body plans with often morphologically and ecologically obscure structures. This poses challenges for accurate and precise identifications. Here we provide a conceptual framework for the identification of fungi, encouraging the approach of integrative (polyphasic) taxonomy for species delimitation, i.e. the combination of genealogy (phylogeny), phenotype (including autecology), and reproductive biology (when feasible). This allows objective evaluation of diagnostic characters, either phenotypic or molecular or both. Verification of identifications is crucial but often neglected. Because of clade-specific evolutionary histories, there is currently no single tool for the identification of fungi, although DNA barcoding using the internal transcribed spacer (ITS) remains a first diagnosis, particularly in metabarcoding studies. Secondary DNA barcodes are increasingly implemented for groups where ITS does not provide sufficient precision. Issues of pairwise sequence similarity-based identifications and OTU clustering are discussed, and multiple sequence alignment-based phylogenetic approaches with subsequent verification are recommended as more accurate alternatives. In metabarcoding approaches, the trade-off between speed and accuracy and precision of molecular identifications must be carefully considered. Intragenomic variation of the ITS and other barcoding markers should be properly documented, as phylotype diversity is not necessarily a proxy of species richness. Important strategies to improve molecular identification of fungi are: (1) broadly document intraspecific and intragenomic variation of barcoding markers; (2) substantially expand sequence repositories, focusing on undersampled clades and missing taxa; (3) improve curation of sequence labels in primary repositories and substantially increase the number of sequences based on verified material; (4) link sequence data to digital information of voucher specimens including imagery. In parallel, technological improvements to genome sequencing offer promising alternatives to DNA barcoding in the future. Despite the prevalence of DNA-based fungal taxonomy, phenotype-based approaches remain an important strategy to catalog the global diversity of fungi and establish initial species hypotheses.

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“…As for phylogenetic analysis for Diaporthe species, the use of a five-locus dataset ( ITS - TUB - TEF - CAL - HIS ) is the optimal combination for species delimitation as revealed by Santos et al (2017) . Thus, in recent years, many Diaporthe species have been described based on a polyphasic approach combined with morphological characterization and their host associations ( Guarnaccia and Crous 2017 ; Gao et al 2017 ; Yang et al 2018 , 2020 ; Crous et al 2020 ; Dayarathne et al 2020 ; Guo et al 2020 ; Hyde et al 2020 ; Li et al 2020 ; Zapata et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species

Sun¹,

Huang²,

Xia³

et al. 2021

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Diaporthe species have often been reported as plant pathogens, endophytes and saprophytes, commonly isolated from a wide range of infected plant hosts. In the present study, twenty strains obtained from leaf spots of twelve host plants in Yunnan Province of China were isolated. Based on a combination of morphology, culture characteristics and multilocus sequence analysis of the rDNA internal transcribed spacer region (ITS), translation elongation factor 1-α (TEF), β-tubulin (TUB), calmodulin (CAL), and histone (HIS) genes, these strains were identified as eight new species: Diaporthe camelliae-sinensis, D. grandiflori, D. heliconiae, D. heterostemmatis, D. litchii, D. lutescens, D. melastomatis, D. pungensis and two previously described species, D. subclavata and D. tectonendophytica. This study showed high species diversity of Diaporthe in tropical rain forests and its hosts in south-western China.

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Taxonomy and phylogeny of hyaline-spored coelomycetes

Cited by 70 publications

References 548 publications

Assessment of Cytospora Isolates From Conifer Cankers in China, With the Descriptions of Four New Cytospora Species

Assessment of Cytospora Isolates From Conifer Cankers in China, With the Descriptions of Four New Cytospora Species

Unambiguous identification of fungi: where do we stand and how accurate and precise is fungal DNA barcoding?

Morphological and molecular identification of Diaporthe species in south-western China, with description of eight new species

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