Taxonomy and phylogeny of Laburnicola gen. nov. and Paramassariosphaeria gen. nov. (Didymosphaeriaceae, Massarineae, Pleosporales)

CoelomycetousDothideomyceteswith emphasis on the familiesCucurbitariaceaeandDidymellaceae

“…The Pleosporales is the largest order of the class Dothideomycetes (phylum Ascomycota ), encompassing more than 4 700 species distributed over 332 genera, and 53 families ( Kirk et al., 2008 , Zhang et al., 2009 , Zhang et al., 2012 , Ariyawansa et al., 2013 , Hyde et al., 2013 , Amaradasa et al., 2014 , Trakunyingcharoen et al., 2014 , Wijayawardene et al., 2014 , Crous et al., 2015a , Sharma et al., 2015 , Tanaka et al., 2015 , Jaklitsch et al., 2016 , Jaklitsch and Voglmayr, 2016 , Wanasinghe et al., 2016 , Crous and Groenewald, 2017 , Hashimoto et al., 2017 , Hernández-Restrepo et al., 2017 ). These fungi are characterised by the production of pseudothecial ascomata (mostly globose and usually papillate) consisting of a peridial wall composed by several layers of cells, within which the fissitunicate (bitunicate) asci are produced amidst a persistent hamathecium (the vegetative structures inside an ascoma) ( Jaklitsch and Voglmayr, 2016 , Jaklitsch et al., 2017 , Zhang et al., 2009 , Zhang et al., 2012 ) and ascospores, which are mostly septate but variable in shape and pigmentation.…”

Neptunomyces aureus gen. et sp. nov. (Didymosphaeriaceae, Pleosporales) isolated from algae in Ria de Aveiro, Portugal

“…The majority of the genera in the Didymosphaeriaceae remain under studied, which makes the family still poorly understood and not well resolved (Wanasinghe et al 2016). In fact, there was no β-tubulin and tef1-α sequence data available for many species and therefore the phylogenetic analyses presented did not encompass all known species of the family.…”

“…So far, species of Didymosphaeriaceae seem to be cosmopolitan in distribution: they have been recorded from both temperate and tropical regions. Also, Didymosphaeriaceae have been found on various hosts and substrates, including plants, humans and soil, being regarded as saprobes, endophytes or pathogens of a wide variety of plant substrates worldwide (Ariyawansa et al 2014a, Liu et al 2015, Wanasinghe et al 2016). However, most Didymosphaeriaceous genera occur on plants of more than 20 host families, the majority of them being monocotyledons and herbaceous plants, such as Anacardiaceae , Asparagaceae , Asteraceae , Caprifoliaceae , Euphorbiaceae , Fagaceae , Lecythidaceae and Poaceae .…”

“…Members of this family are characterized by having brown 1-septate ascospores and trabeculate pseudoparaphyses that anastomose mostly above the asci (Aptroot 1995, Hyde et al 2013, Ariyawansa et al 2014a, b). Species of Didymosphaeriaceae are saprobes, endophytes or pathogens of a wide variety of plant species worldwide (Ariyawansa et al 2014a, Liu et al 2015, Wanasinghe et al 2016).…”

“…First, Ariyawansa et al (2014a) showed that Montagnulaceae and Didymosphaeriaceae were synonyms and thus, Ariyawansa et al (2014b) synonymized Montagnulaceae under Didymosphaeriaceae and rearranged the family into 16 genera: Alloconiothyrium , Barria , Bimuria , Deniquelata , Didymocrea , Didymosphaeria , Julella , Kalmusia , Karstenula , Letendraea , Montagnula , Neokalmusia , Paraconiothyrium , Paraphaeosphaeria , Phaeodothis and Tremateia . Subsequently, in the last years, additional genera were added, namely Paracamarosporium and Pseudocamarosporium (Wijayawardene et al 2014), Spegazzinia (Tanaka et al 2015), Xenocamarosporium (Crous et al 2015), Austropleospora and Pseudopithomyces (Ariyawansa et al 2015) and Laburnicola and Paramassariosphaeria (Wanasinghe et al 2016). More recently, Jayasiri et al (2019) introduced Cylindroaseptospora and Gonçalves et al (2019) reassigned the genus Verrucoconiothyrium previously included in the family Didymosphaeriaceae to the family Didymelaceae .…”