Taxonomy and Systematics

Stackebrandt, Erko; Hippe, Hans

doi:10.1002/3527600108.ch2

Cited by 28 publications

(21 citation statements)

References 75 publications

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“…Endospores are oval, central to subterminal. Cells stain Gram-negative but are phylogenetically Gramtype-positive (Wiegel, 1981), belonging to the phylum (Newman et al, 1997;Stackebrandt et al, 2003); 3, Desulfosporosinus meridiei S10 T (Robertson et al, 2001); 4, Desulfosporosinus orientis DSM 765 T (Campbell & Postgate, 1965;Stackebrandt et al, 2003); 5, Desulfosporosinus lacus STP12 T (Ramamoorthy et al, 2006); 6, Desulfosporosinus hippei 343 T (Vatsurina et al, 2008 The predominant whole-cell fatty acids are C 16 : 1 (~58 %) and C 16 : 0 (~22 %). The genomic DNA G+C content of the type strain is 36.6 mol% (HPLC).…”

Section: Description Of Desulfosporosinus Youngiae Sp Novmentioning

confidence: 99%

Desulfosporosinus youngiae sp. nov., a spore-forming, sulfate-reducing bacterium isolated from a constructed wetland treating acid mine drainage

Lee

Romanek

Wiegel

2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Strain JW/YJL-B18 T , a spore-forming, sulfate-reducing bacterium, was isolated from constructed wetland sediment. Cells were curved rods, 0.7-1.2 mm in diameter and 3-7 mm long. Despite being phylogenetically a member of the Gram-type-positive phylum Firmicutes, cells stained Gram-negative at all growth phases. Strain JW/YJL-B18 T grew at 8-39 6C, with an optimum at 32-35 6C and no growth at 4 6C or below or at 42 6C or above. The pH 25 6C range for growth was 5.7-8.2, with an optimum at pH 25 6C 7.0-7.3, and no growth was detected at or below pH 5.2 or at or above pH 8.4. The salinity range for growth was 0-3 % (NaCl/KCl 9 : 1). Strain JW/YJL-B18 T utilized as carbon and energy sources beef extract, yeast extract, formate, succinate, lactate, pyruvate, ethanol and toluene. Fumarate, sulfate, sulfite and thiosulfate were reduced in the presence of lactate. Arsenate (V) was not used as an electron acceptor. Strain JW/YJL-B18 T showed no indication of growth under autotrophic conditions. The predominant cellular fatty acids were C 16 : 1 and C 16 : 0 . The genomic DNA G+C content was 36.6 mol% (HPLC). 16S rRNA gene sequence analysis indicated that strain JW/YJL-B18 T fell into the genus Desulfosporosinus, with Desulfosporosinus auripigmenti OREX-4 T as its closest neighbour with a validly published name (97.9 % similarity). Based on molecular genetic evidence and physiological and biochemical characters including differences in the DNA G+C content, we propose to place strain JW/YJL-B18 T (5DSM 17734 T 5ATCC BAA-1261 T ) as the type strain of a novel species, Desulfosporosinus youngiae sp. nov.Sulfate-reducing bacteria (SRB) are responsible for a variety of biogeochemical processes. For example, SRB reduce sulfate to dissolved sulfide (S 22 ), which may react with metals to form metal sulfides; thus, they are considered to play a major role in metal removal and alkalinity generation in treating acid mine drainage. The SRB are an ecologically and metabolically diverse group of anaerobes which include members of the phylum Firmicutes (formerly the phylogenetically Gram-type-positive bacteria; Wiegel, 1981) residing within the family Peptococcaceae in the order Clostridiales. Among them, the genus Desulfosporosinus currently consists of five species of obligately anaerobic, spore-forming bacteria (http:// www.bacterio.cict.fr/d/desulfosporosinus.html). Stackebrandt et al. (1997) distinguished this group from the genus Desulfotomaculum on the basis of phylogenetic evidence and their ability to grow under autotrophic conditions. However, the property of autotrophic growth (i.e. 50 % of the biomass is derived from inorganic carbon) has not been demonstrated unequivocally for all species. Vatsurina et al. (2008) recently reported Desulfosporosinus orientis 343 as the type strain of a novel species in the same genus, Desulfosporosinus hippei, which does not oxidize H 2 with CO 2 or acetate. Here, we report another novel Desulfosporosinus species that is not capable of growing lithotrophically, isolated from a construc...

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Section: Description Of Desulfosporosinus Youngiae Sp Novmentioning

confidence: 99%

Desulfosporosinus youngiae sp. nov., a spore-forming, sulfate-reducing bacterium isolated from a constructed wetland treating acid mine drainage

Lee

Romanek

Wiegel

2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…These data, considered in conjunction with strain-specific differences in heavy metal tolerance, cell-wall chemotaxonomy and riboprint patterns, support recognition of strain STP12 T (=DSM 15449 T =JCM 12239 T ) as the type strain of a distinct and novel species within the genus Desulfosporosinus, Desulfosporosinus lacus sp. nov.The genus Desulfosporosinus currently contains three species with validly published names, Desulfosporosinus orientis (Stackebrandt et al, 1997), Desulfosporosinus auripigmenti (Stackebrandt et al, 2003) and Desulfosporosinus meridiei (Robertson et al, 2001). Isolates from locales separated by thousands of kilometres exist for some of these species (e.g.…”

mentioning

confidence: 99%

“…The genus Desulfosporosinus currently contains three species with validly published names, Desulfosporosinus orientis (Stackebrandt et al, 1997), Desulfosporosinus auripigmenti (Stackebrandt et al, 2003) and Desulfosporosinus meridiei (Robertson et al, 2001). Isolates from locales separated by thousands of kilometres exist for some of these species (e.g.…”

mentioning

confidence: 99%

“…Physiological attributes of strain STP12 T were evaluated in parallel with those of other members of the genus Desulfosporosinus. D. meridiei DSM 13257 T (Robertson et al, 2001), D. orientis DSM 765 T (Campbell & Postgate, 1965) and D. auripigmenti DSM 13351 T (Newman et al, 1997;Stackebrandt et al, 2003) were obtained from the DSMZ. Bacteria were initially cultivated on the appropriate recommended DSMZ medium.…”

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confidence: 99%

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Desulfosporosinus lacus sp. nov., a sulfate-reducing bacterium isolated from pristine freshwater lake sediments

Ramamoorthy

Sass

Langner

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

109

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A novel sulfate-reducing bacterium was isolated from pristine sediments of Lake Stechlin, Germany. This strain, STP12 T , was found to contain predominantly c-type cytochromes and to reduce sulfate, sulfite and thiosulfate using lactate as an electron donor. Although STP12 T could not utilize elemental sulfur as an electron acceptor, it could support growth by dissimilatory Fe(III) reduction. In a comparison of 16S rRNA gene sequences, STP12 T was 96?7 % similar to Desulfosporosinus auripigmenti DSM 13351 T , 96?5 % similar to Desulfosporosinus meridiei DSM 13257 T and 96?4 % similar to Desulfosporosinus orientis DSM 765 T . DNA-DNA hybridization experiments revealed that strain STP12 T shows only 32 % reassociation with the type strain of the type species of the genus, D. orientis DSM 765 T . These data, considered in conjunction with strain-specific differences in heavy metal tolerance, cell-wall chemotaxonomy and riboprint patterns, support recognition of strain STP12 T (=DSM 15449 T =JCM 12239 T ) as the type strain of a distinct and novel species within the genus Desulfosporosinus, Desulfosporosinus lacus sp. nov.The genus Desulfosporosinus currently contains three species with validly published names, Desulfosporosinus orientis (Stackebrandt et al., 1997), Desulfosporosinus auripigmenti (Stackebrandt et al., 2003) and Desulfosporosinus meridiei (Robertson et al., 2001). Isolates from locales separated by thousands of kilometres exist for some of these species (e.g. DSM 8344; Vainshtein et al., 1994). Culture-and nonculture-based studies have revealed the existence of Desulfosporosinus-like organisms in environmental settings as diverse as permafrost (Vainshtein et al., 1994), pristine aquifers (Detmers et al., 2004), municipal drinking water (Bade, 2000) and rice plant roots (Scheid et al., 2004).Multiple lines of evidence suggest that members of the genus also commonly inhabit industrially impacted soils and sediments. D. auripigmenti was isolated from an arseniccontaminated watershed, and is one of a limited number of bacteria known to respire arsenate (Newman et al., 1997). Recently, another arsenic-reducing Desulfosporosinus strain was isolated from arsenic-contaminated sediments; this organism has the capacity to metabolize a wide variety of aromatic compounds (Liu et al., 2004). Kusel et al. (2001) reported the isolation of Desulfosporosinus-like organisms from a coal-mining-impacted lake, while Robertson et al. (2000) isolated eight Desulfosporosinus strains from a hydrocarbon-contaminated soil. Two of these were later described as D. meridiei (Robertson et al., 2001) and their activity was linked to toluene mineralization (Robertson et al., 2000;Franzmann et al., 2002 et al., 2003), and uranium mine tailings in Shiprock, NM (Nevin et al., 2003). Significantly, enrichment of Desulfosporosinus-like microbes has been associated with stimulated removal of solution-phase uranium (Nevin et al., 2003). In such contexts, uranium precipitation may occur secondarily to sulfidogenesis or as a primary resul...

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“…At the time of writing, it comprised seven species of obligately anaerobic, sporeforming bacteria with validly published names: Desulfosporosinus orientis, the type species of the genus Desulfosporosinus, (Stackebrandt et al, 1997), D. auripigmenti (Stackebrandt et al, 2003), D. meridiei (Robertson et al, 2001), D. lacus (Ramamoorthy et al, 2006) D. hippei (Vatsurina et al, 2008) D. youngiae (Lee et al, 2009) and D. acidiphilus (Alazard et al, 2010). Desulfosporosinus-like organisms are widespread in different habitats.…”

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confidence: 99%

Desulfosporosinus burensis sp. nov., a spore-forming, mesophilic, sulfate-reducing bacterium isolated from a deep clay environment

Mayeux

Fardeau

Bartoli-Joseph

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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Desulfosporosinus burensis sp. nov., a spore-forming, mesophilic, sulfate-reducing bacterium isolated from a deep clay environment A novel anaerobic, Gram-positive, spore-forming, curved rod-shaped, mesophilic and sulfatereducing bacterium was isolated from pore water collected in a borehole at "490 m in Bure (France). This strain, designated BSREI1 T , grew at temperatures between 5 6C and 30 6C (optimum 25 6C) and at a pH between 6 and 8 (optimum 7). It did not require NaCl for growth, but tolerated it up to 1.5 % NaCl. Sulfate, thiosulfate and elemental sulfur were used as terminal electron acceptors. Strain BSREI1 T used crotonate, formate, lactate, pyruvate, fructose, glycerol and yeast extract as electron donors in the presence of sulfate. The sole quinone was MK-7. The G+C content of the genomic DNA was 43.3 mol%. Strain BSREI1 T had the type strains of Desulfosporosinus lacus (16S rRNA gene sequence similarity of 96.83 %), Desulfosporosinus meridiei (96.31 %) and Desulfosporosinus hippei (96.16 %) as its closest phylogenetic relatives. On the basis of phylogenetic and physiological properties, strain BSREI1 T is proposed as a representative of a novel species of the genus Desulfosporosinus, Desulfosporosinus burensis sp. nov.; the type strain is BSREI1 T (5DSM 24089 T 5JCM 17380 T ).

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Taxonomy and Systematics

Cited by 28 publications

References 75 publications

Desulfosporosinus youngiae sp. nov., a spore-forming, sulfate-reducing bacterium isolated from a constructed wetland treating acid mine drainage

Desulfosporosinus youngiae sp. nov., a spore-forming, sulfate-reducing bacterium isolated from a constructed wetland treating acid mine drainage

Desulfosporosinus lacus sp. nov., a sulfate-reducing bacterium isolated from pristine freshwater lake sediments

Desulfosporosinus burensis sp. nov., a spore-forming, mesophilic, sulfate-reducing bacterium isolated from a deep clay environment

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